Iba1 (AIF1) (NM_004847) Human Untagged Clone

CAT#: SC108919

AIF1 (untagged)-Human allograft inflammatory factor 1 (AIF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AIF1
• Synonyms: AIF-1; IBA1; IRT-1; IRT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004847, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGTTTGACCTTAATGGAAATGGCGATATTGGTGAGAAACGGGTGATTTGCGGGGGCAGGGTGGTGT
  GCAGGCCTAAGAAGACAGAGGTCTCTCCTACATGCTCCATTCCTCATGATTTGGGAGGGGGCCCACCTAC
  CACAGTGGGAGGAAGGAGAATGGGGATGCGGAAGTGGGAGAGGAGAGAGAGGGTCTCCCCACCTTCTCCC
  CATCCCCATCCTCTGCCCCCAGATATCATGTCCCTGAAACGAATGCTGGAGAAACTTGGAGTCCCCAAGA
  CTCACCTAGAGCTAAAGAAATTAATTGGAGAGGTGTCCAGTGGCTCCGGGGAGACGTTCAGCTACCCTGA
  CTTTCTCAGGATGATGCTGGGCAAGAGATCTGCCATCCTAAAAATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004847 unedited
  TTAAATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACCAGNAGGCCTCCAGCT
  TGGTCTGTCTCCCCACCTCTACCAGCATCTGCTGAGCTATGAGCCAAACCAGGGATTTAC
  AGGGAGGAAAAGCTTTCGGACTGCTGAAGGCCCAGCAGGAAGAGAGGCTGGATGAGATCA
  ACAAGCAATTCCTAGACGATCCCAAATATAGCAGTGATGAGGATCTGCCCTCCAAACTGG
  AAGGCTTCAAAGAGAAATACATGGAGTTTGACCTTAATGGAAATGGCGATATTGATATCA
  TGTCCCTGAAACGAATGCTGGAGAAACTTGGAGTCCCCAAGACTCACCTAGAGCTAAAGA
  AATTAATTGGAGAGGTGTCCAGTGGCTCCGGGGAGACGTTCAGCTACCCTGACTTTCTCA
  GGATGATGCTGGGCAAGAGATCTGCCATCCTAAAAATGATCCTGATGTATGAGGAAAAAG
  CGAGAGAAAAGGAAAAGCCAACAGGCCCCCCAGCCAAGAAAGCTATCTCTGAGTTGCCCT
  GATTTGAAGGGAAAAGGGATGATGGGATTGAAGGGGCTTCTAATGACCCAGATATGGAAA
  CAGAAGACAAAATTGTAAGCCAGAGTCAACAAATTAAATAAATTACCCCCTCCTCCAGAT
  CAAAAAAAACTCTAGATTGCNGCCGCGGTCATAGCTGTTTTCTGAACAGATCCCCGGGTG
  GCATCCCTGTGACCCCTTCCCAGTGCCTTTCCTGCCCTGGAAGTTGCCACTCCAGTGCCC
  ACCACCTTGTCCTATAAAATTAAGTGCATCATTTTGCTGACTAGTGTCCTCTTATAATAA
  TGGGGGAGGGGGGGGGGGGTTTNNTTTTGCTTNNNATNATTTNTTTTNNTGTATTTTTTT
  TTNTTGTGAGTTNTTTTTTTATCTTGTTTTATTCTTCGTCTTTCTCCCCTTTCCTGACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004847 unedited
  CGGCCGCAATCTAGAATTTTTTTTGATCTGGAGGAGGGGTAATTTATTTAATTTGTTGAC
  TCTGGCTTACAATTTTGTCTTCTGTTTCCATATCTGGGTCATTAGAAGCCCCTTCAATCC
  CATCATCCCTTTTCCCTTCAAATCAGGGCAACTCAGAGATAGCTTTCTTGGCTGGGGGGC
  CTGTTGGCTTTTCCTTTTCTCTCGCTTTTTCCTCATACATCAGGATCATTTTTAGGATGG
  CAGATCTCTTGCCCAGCATCATCCTGAGAAAGTCAGGGTAGCTGAACGTCTCCCCGGAGC
  CACTGGACACCTCTCCAATTAATTTCTTTAGCTCTAGGTGAGTCTTGNGGACTCCAAGTT
  TCTCCAGCATTCGTTTCAGGGACATGATATCAATATCGCCATTTCCATTAAGGTCAAACT
  CCATGTATTTCTCTTTGAAGCCTTCCAGTTTGGAGGGCAGATCCTCATCACTGCTATATT
  TGGGATCGTCTAGGAATTGCTTGTTGATCTCATCCAGCCTCTCTTCCTGCTGGGCCTTCA
  GCAGTCCGAAAGCTNNTCCTNCCCTGTAATCCCCTGTTTGGCTCATACCTCANCAGATGC
  TGGTAGAGGTGGNGAGACAGACCAAAGCTGGAGCCTNNCTGTGCCCGATCGCNGCCGCCC
  TATAGTGAGTCGTATTACANAATTCTGACGGNTCACTAAACGAGCTCTGCTTATATAGAC
  CTCCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGGGCGGGGTATTACGACATTT
  TGGAAAGTCCGTTGATTTTGGTGCCAACAAACTCCATTGACGTCAAGGGGTGGAAACTTG
  GAAATCCCGTGAGTCAACCCGCTTCCAGCCCATTGGTGTACTGNCAAAACCGCATACCAT
  GTAATACGAGACTATCCCTAAATGACTGCAGTAGGAAGTCCGTAAGTCATGTCTGGCATA
  TGGCAGCGGCCATTACGGTATGACGTAATAGGGCGACTTGCTAGATCATTGAGACTGCAA
  TG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004847
• Insert Size: 560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004847.2, NP_004838.1
• RefSeq Size: 1491 bp
• RefSeq ORF: 1491 bp
• Locus ID: 199
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and retains introns as compared to variant 3. The resulting isoform (2) is shorter at the N- and C-termini and differs internally compared to isoform 3.