NUDT2 (NM_147172) Human Untagged Clone

CAT#: SC108939

NUDT2 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 2 (NUDT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUDT2
• Synonyms: APAH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_147172 edited
  ATGGCCTTGAGAGCATGTGGCTTGATCATCTTCCGAAGATGCCTCATTCCCAAAGTGGAC
  AACAATGCAATTGAGTTTTTACTGCTGCAGGCATCAGATGGCATTCATCACTGGACTCCT
  CCCAAAGGCCATGTGGAACCAGGAGAGGATGACTTGGAAACAGCCCTGAGGGAGACCCAA
  GAGGAAGCAGGCATAGAAGCAGGCCAGCTGACCATTATTGAGGGGTTCAAAAGGGAACTC
  AATTATGTGGCCAGGAACAAGCCTAAAACAGTCATTTACTGGCTGGCGGAGGTGAAGGAC
  TATGACGTGGAGATCCGCCTCTCCCATGAGCACCAAGCCTACCGCTGGCTGGGGCTGGAG
  GAGGCCTGCCAGTTGGCTCAGTTCAAGGAGATGAAGGCAGCGCTCCAAGAAGGACACCAG
  TTTCTTTGCTCCATAGAGGCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_147172 unedited
  TCGGACCAGACCATGGCCTTGAGAGCATGTGGCTTGATCATCTTCCGAAGATGCCTCATT
  CCCAAAGTGGACAACAATGCAATTGAGTTTTTACTGCTGCAGGCATCAGATGGCATTCAT
  CACTGGACTCCTCCCAAAGGCCATGTGGAACCAGGAGAGGATGACTTGGAAACAGCCCTG
  AGGGAGACCCAAGAGGAAGCAGGCATAGAAGCAGGCCAGCTGACCATTATTGAGGGGTTC
  AAAAGGGAACTCAATTATGTGGCCAGGAACAAGCCTAAAACAGTCATTTACTGGCTGGCG
  GAGGTGAAGGACTATGACGTGGAGATCCGCCTCTCCCATGAGCACCAAGCCTACCGCTGG
  CTGGGGCTGGAGGAGGCCTGCCAGTTGGCTCAGTTCAAGGAGATGAAGGCAGCGCTCCAA
  GAAGGACACCAGTTTCTTTGCTCCATAGAGGCCTGAGCTGACTGGAGCAGAGTCATTTGC
  TTCAGCAGGATCCTTGTGGGCCTTCTAGATGAAGCCACCCTCAGGTCCAGGGAAGGGTGT
  GCTGGTATTTGGCTCATGACAGCCAAGAGCAGATTTGTGAAATCGGCTCAACTCCCAGGT
  GAGAGCAAGCANAAATCTTGGCTGGGGTGGAAGGAAGGCAAAAGAGTAAAAATTAAAAAG
  CCAGGCCCAGTAAGTGTACCCTGTACTTTATAATTAAANTCAAGCAGCTCCAAANAAAAA
  ANNANNNNNNAAAANNANNAAAAAAAANAAAAAAAAAAAAAAAAAAAAACCCCCTCCCTC
  TA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_147172
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147172.1, NP_671701.1
• RefSeq Size: 989 bp
• RefSeq ORF: 444 bp
• Locus ID: 318
• Cytogenetics: 9p13.3
• Domains: NUDIX
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) uses an alternate splice site on a 5' non-coding exon, compared to variant 1. All four variants encode the same protein.