ASAH1 (NM_004315) Human Untagged Clone

CAT#: SC108952

ASAH1 (untagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAH1
• Synonyms: AC; ACDase; ASAH; PHP; PHP32; SMAPME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004315, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACTGCTGCATCGGGCTGGGAGAGAAAGCTCGCGGGTCCCACCGGGCCTCCTACCCAAGTCTCAGCG
  CGCTTTTCACCGAGGCCTCAATTCTGGGATTTGGCAGCTTTGCTGTGAAAGCCCAATGGACAGAGGACTG
  CAGAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTTCCATGGTACACCATAAATCTT
  GACTTACCACCCTACAAAAGATGGCATGAATTGATGCTTGACAAGGCACCAGTGCTAAAGGTTATAGTGA
  ATTCTCTGAAGAATATGATAAATACATTCGTGCCAAGTGGAAAAATTATGCAGGTGGTGGATGAAAAATT
  GCCTGGCCTACTTGGCAACTTTCCTGGCCCTTTTGAAGAGGAAATGAAGGGTATTGCCGCTGTTACTGAT
  ATACCTTTAGGAGAGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAG
  CAGAAGACAAAAAAGGTCATCTAATACATGGGAGAAACATGGATTTTGGAGTATTTCTTGGGTGGAACAT
  AAATAATGATACCTGGGTCATAACTGAGCAACTAAAACCTTTAACAGTGAATTTGGATTTCCAAAGAAAC
  AACAAAACTGTCTTCAAGGCTTCAAGCTTTGCTGGCTATGTGGGCATGTTAACAGGATTCAAACCAGGAC
  TGTTCAGTCTTACACTGAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATTCTAGAATGGATTCT
  GGGAAAGAAAGATGTCATGTGGATAGGGTTCCTCACTAGAACAGTTCTGGAAAATAGCACAAGTTATGAA
  GAAGCCAAGAATTTATTGACCAAGACCAAGATATTGGCCCCAGCCTACTTTATCCTGGGAGGCAACCAGT
  CTGGGGAAGGTTGTGTGATTACACGAGACAGAAAGGAATCATTGGATGTATATGAACTCGATGCTAAGCA
  GGGTAGATGGTATGTGGTACAAACAAATTATGACCGTTGGAAACATCCCTTCTTCCTTGATGATCGCAGA
  ACGCCTGCAAAGATGTGTCTGAACCGCACCAGCCAAGAGAATATCTCATTTGAAACCATGTATGATGTCC
  TGTCAACAAAACCTGTCCTCAACAAGCTGACCGTATACACAACCTTGATAGATGTTACCAAAGGTCAATT
  CGAAACTTACCTGCGGGACTGCCCTGACCCTTGTATAGGTTGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004315 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGGATGGCAC
  GAAAGCCAAGCGAGTCCCCCTGCCGAGCTACTCGCGTCCGCCTCCTCCCAAGCTGAGCTC
  TGCTCCGCCCACCTGAGTCCTTCGCCAGTTAGGAGGAAACACAGCCGCTTAATGAACTGC
  TGCATCGGGCTGGGAGAGAAAGCTCGCGGGTCCCACCGGGCCTCCTACCCAAGTCTCAGC
  GCGCTTTTCACCGAGGCCTCAATTCTGGGATTTGGCAGCTTTGCTGTGAAAGCCCAATGG
  ACAGAGGACTGCAGAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTT
  CCATGGTACACCATAAATCTTGACTTACCACCCTACAAAAGATGGCATGAATTGATGCTT
  GACAAGGCACCAATGCTAAAGGTTATAGTGAATTCTCTGAAGAATATGATAAATACATTC
  GTGCCAAGTGGAAAAGTTATGCAGGTGGTGGATGAAAAATTGCCTGGCCTACTTGGCAAC
  TTTCCTGGCCCTTTTGAAGAGGAAATGAAGGGTATTGCCGCTGTTACTGATATACCTTTA
  GGAGAGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTTGTACTTCAATAGT
  AGCAGAAGAAAAAAAGGTCATCTAATACATGGGAGAAACATGGNATTTGGAGTATTTCTT
  GGGTGGAACATANATAATGATACCCTGNGTCATAACTGAGCAAACTAAACCTTTACAGTG
  AATNTGGATTNCAAAAGAAACACANNACTGTCTCAGGCTTCAAGCTTTGCTGCTATGNGG
  GCATGTTACAGGATTTCAACAGACTGTTCAGTCTAACTGAATGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004315 unedited
  GCTCTTGCTGTCTGTTTCCTGAGTTGTCAGGGAACACAACTGTGCATTATACAAAATGAA
  GTGGACAAACGGTCTTGCACAAATGACGTACATTTCACAGTTAATCGTGAAGGTTAGAAA
  GGTTAACAAGTTCTATTAGTACTTTTCATAAGCAGTTGAGATTTCTAGAAAAATACAGTA
  ACATAATTTTAGTAACTACTAAAATTTCTTTAAAACTCCCTATCAATCTGAAGAACAAAC
  TAAAAACCTGTTTATTACATGAATGCTACTTATGAGAATTTAAAATATGGGTTCACTTCC
  TATTTTCCACAAGTCTTTGACTTGTTTATTTACTAAATTAACAGAACGTGGGATGCAGTT
  TTTTAAGTTAGCAACTTCCTTAGGGTTACTGGGAAGTAGAGTAATTATGAAAAAGGGAAC
  ACTTCAATTATTGTATAAAAGATTGCTTTGCATACTGATTACTTCTTGAACCCCAAAAAG
  TGCTATTTAGAGGCAAGGGCCCATTTTGTGCCCATTAAAAGAAATATACAGGTGAATGTC
  TGTCTCATCCCTCTAATCATATGTAATAAAATTATATCGCTGTCAATAACAGCAAAGATA
  ATGATATATTAATTTTAACAGCAGTTAGAACCAGAAGGAAAAGGCTGTTATACAGAAAAA
  CATACAAATATGTAAGTAGAAGTGAAAAACTGAAGAATGTGGAGTGTTTACTGTCCCGTT
  ACTCACACAGACGTGCTGGATTCAACACCCACGCTGAAATCTCATTTATTCCACGGGAGT
  GGAGTTCACCATGGTTCGACTGCCCACCANATTCCCCAATGTCAAAGTGAAACAAAACTC
  AGTGAATTCTAAATGACTGTTTTACTTCCCCTAAAGAAGTTATCTGGTAATTAGAAAAAA
  TCACTGATAGGGGGGAAAAAAAAGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004315
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004315.2, NP_004306.2
• RefSeq Size: 2503 bp
• RefSeq ORF: 1236 bp
• Locus ID: 427
• Cytogenetics: 8p22
• Domains: CBAH
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: 'This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (2) encodes the longest isoform (b).