Calretinin (CALB2) (NM_007087) Human Untagged Clone

CAT#: SC108995

CALB2 (untagged)-Human calbindin 2 (CALB2), transcript variant CALB2b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALB2
• Synonyms: CAL2; calbindin 2; calbindin 2, (29kD, calretinin); calbindin D29K; calretinin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007087, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCTGGAAA
  TATGGAAGCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGAAAACTTTTTCCAAGA
  GCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGACAACTTTGGAGAAAAGATGAAGGAG
  TTCATGCAGAAGTATGATAAAAACTCAGATGGGAAAATCGAGATGGCAGAGCTGGCGCAGATCCTGCCAA
  CCGAAGAGAACTTCCTTCTGTGCTTCAGGCAGCACGTGGGCTCCAGCGCCGAGTTTATGGAGGCTTGGCG
  GAAGTACGACACAGACAGGAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTG
  AAGAAGGCGAACCGGCCGTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTACGGATGTTTG
  ACTTGAACGGGGATGGCAAATTGGGCCTCTCAGAGATGTCCCGGGCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007087 unedited
  TCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCCAGCCGGCG
  CGGAGCGGGAGCGGTGCAGGCTGAGGTCTCCGAGCGGCTCGCCATGGCTGGCCCGCAGCA
  GCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCTGGAAATATGGAA
  GCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGAAAACTTTTTCCA
  AGAGCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGACAACTTTGGAGA
  AAAGATGAAGGAGTTCATGCAGAAGTATGATAAAAACTCAGATGGGAAAATCGAGATGGC
  AGAGCTGGCGCAGATCCTGCCAACCGAAGAGAACTTCCTTCTGTGCTTCAGGCAGCACGT
  GGGCTCCAGCGCCGAGTTTATGGAGGCTCGGCGGAAGTACGACACAGACAGGAGTGGCTA
  CATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTGAAGAAGGCGAACCGGCC
  GTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTACGGATGTTTGACTTGAA
  CGGNGATGGCAAATTGGGCCTCTCAGAGATGTCCCGACTCCTGCCTGTCCAGGAAAACTT
  CCTGCTTAAATTTCAGGGCATGAAGCTGACCTCAGAGGAGTTTAACGCGATCTTCACATT
  TTACGACAAGGATAGAAGCGGCTACATTGACGAGCATGAGCTGGATGCCCTTTTGAAGGA
  TCTGTACGAGAAAAACANANAGGAAATGAATATTCAACAGCTCACCAACTACAGAAAGAG
  CGTCATGTCCTTGGGCAGAGCAGGNAAGCTCTACCGCAAGGGACCTGNNAGATGTGCTCT
  GCAGCGAGCCCCCCATGTAAGGGGGGACGGGGGCTGGTNNNTNNCACTNCCCAAANCCCT
  GCTNTGCTGNCCTGATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007087 unedited
  CGCAATCTAGAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGTCCCAAAAAAGGACCTTTCCATTTTATT
  GGCGGAAAACTCTTTTTTGGGAAACAAAAAACCCAAAAAAAAAAGGGCCCCCCCCATTAA
  AAAAGGCAAGGAAAGGGGGGGGGCCTTCCACACACAGGATTTTTGAAAGGACACCTCCCC
  CCTTGGCAAGGGGGGGGATGGAACCCGAAATTTTGGGGGGGGGGGGGGCATGGGGGACCC
  GCAAAGGGGACCCAAAGGGAAAATTCCCCTTTGTGGGACATAAAACCCTAAATCATACCG
  GGGGGGAGCTCATGCCTCACAGCGAAAAAAATTAGGGACCCTCCACCCATGCCCCCATAA
  CCTTTCATGGGGGGGACCCACTGAACAAAAACTGCCTGGGCAGGGGGGGGTGCAAGGGGC
  TAAAGAAAACCCCCCATTTGCCCATCCTTTATTTTTTGTTTCCTGCCCTGAAAGCAGGTG
  TTTCCAAGCTGTAAGGGTGGGGGCGGGGCGCTCACGGTCTTTGATTCTGGGTAAACCCCT
  CAAGGCAACAAAACCAGGGTTTGGGGGAGGGGGAAAATCACCCCCCTCCCCTCTTAATAG
  GGGGCTCCCTGAAAAAACAATATCCAAGTCCTTGGGGGAAAACTCCCCTCCCCTTGCAAA
  GAAAAAACCTTTTTTTGAATGGGGGAGACGGAAAATTCATTTCCTTTTGGTTTTTTCGAA
  AACCCTTAAAGGCACCCCCTCTTCCCCAAAGGTCCCCTTCTTTCTTGGGAAAAAAGGAAA
  AACCGGAAAACCCCCGGAGGGCCTTTTCCCTCAAATTAACAGAAATTTCCTGCAAGCAGA
  GATGGAATTCTTGAAGCCCAATTGCCTCCCGTCAAAAAACCCAAATGGGTGGGGGTTCCT
  GAACCGGGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007087
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007087.1, NP_009018.1
• RefSeq Size: 1413 bp
• RefSeq ORF: 540 bp
• Locus ID: 794
• Cytogenetics: 16q22.2
• Domains: EFh
• Gene Summary: 'This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (CALB2b) lacks an alternate segment in the 3' coding region which results in a frameshift and an early stop codon, compared to variant 1. The resulting protein (isoform 20k) has a shorter and distinct N-terminus when it is compared to isoform 1.