Caldesmon (CALD1) (NM_004342) Human Untagged Clone

CAT#: SC108996

CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALD1
• Synonyms: CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108996 sequence for NM_004342 edited (data generated by NextGen Sequencing)
  ATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAAGAGGGAGGAGATGCGACTC
  GAAGCAGAAAGAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAA
  CGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGA
  CAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAG
  ACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTG
  GCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAG
  TTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGAC
  ACAGCAGAAAATGAAACTACCGAGAAGAAAGAAAAAAGTGAAAGTCGCCAAGAAAGATAC
  GAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCT
  GAAGAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGA
  GGGAGCATTGGAGAAAATCAGATCAAAGATGAAAAGATTAAAAAGGACAAAGAACCCAAA
  GAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTTACAGAAGTTAAGTCGCAGAAT
  GGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACTTTCAGCCGCCCTGGAGGG
  AGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGG
  CTGGAGGAGCTTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAA
  CAGAAGCAGCAGGAGGCGGCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGA
  AGGAAGGTCCTGGAGGAGGAAGAGCAGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTC
  AGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGAGATTGAAAGGCGAAGAGCAGAAGCT
  GCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCAGATGACAAGAAACCATTCAAG
  TGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGAATAAG
  TCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATT
  GACAGCAGACTGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACA
  AAGCCGGCAGCCTCGGATCTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATG
  TGGGAGAAAGGGAATGTGTTTTCATCCCCCACTGCAGCAGGCACACCAAATAAGGAAACT
  GCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAATGAATGGCTAACTAAAACCCCAGAT
  GGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGAGACGTATCCAGCAAG
  CGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTTTGA
  
  Clone variation with respect to NM_004342.6
  448 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004342 unedited
  TACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCTGCTTGCTCTCTGGCT
  GTGCTCCTGCTTAAAGAAATCAGTCCTTCCTTTCCGACTTAGTCCTCGGGAAGAAGTTTC
  AGACTACAAGGTATCATTGGAACATTTCAAGATCATCAAATCAAATTCCACAGGGATTGG
  TGACCAACCAGAAGGCTCAGACATCTGATTGCTGACCTGTCCAGACATCATCTGGTCTCC
  CTGAACCTGAAATCACACCATGGATGATTTTGAGCGTCGCAGAGAACTTAGAAGGCAAAA
  GAGGGAGGAGATGCGACTCGAAGCAGAAAGAATCGCCTACCAGAGGAATGACGATGATGA
  AGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCA
  GGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGTGT
  GCCTGACGAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGGC
  CGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGC
  TCTGGAGCGGCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAG
  CAGAAGAATGCANAATGACACAGCAGAAAATGAAACTACCGAGAAGAAAGAAAAAGTGAA
  AGTCGCCAAGAAAGATACGAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAGAAG
  AATGATGGAGGAATGCTGAANAANNACAGAAAGAAACAGGNNAAAGGAGGAGGAGAAAGA
  GAGAAGCCAAGCGAGGGAGCTTGGNNNGAAATCNGATCAAGATGAAAGATAAAAGGGCCA
  AGAACCCAAGAAGAAGTTAGAGCTCCATGGATCGAAAGAAGGGATTTCAGAAGTTTAGTC
  CAGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004342 unedited
  CGCGGCCGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAAAGACACCATTATTGCAG
  TATTTCAAATCTGAAATGGGTTACTTTCAAAAGTGTTACAATTGCCTACTAAGATAATTT
  CTTCCATGCTTTATTATAAAGTGCAGAAACAACATGACTTCTGTATTTAAAAAAACAAAA
  ACTACGGTTCATTTTTCTAGATACTGCACACATTCCGCAGGCAATTTTAAACTTGGATCT
  TCTGTTGACTTCAGATGTGGTTGGTATCACTGCTCAGATACAGAGTTATGATGATCAGTA
  GAAAAGTCTCTATTTCACAGCATGGGTTTCTTTAAAAACAGGCTCCTGTGCAAAGGCAGT
  ACTTTTACCATGAACATCTCTAGACTGTGATTATTAAATATAGTGATAATATACATGGGT
  TTACTGGGATATTGAAAAATAAAAGATAATGAACCCAATTTAGTAAATCAACATAAATAC
  AAAACAGAGCGAATTAGCCCTCTACAACTGAGCTCGTCCTGCGTCTTGAGCTTGGGTTCT
  TTCTGGAACTGTCTCAAACCTTAGTGGGGGAAGTGACCTTATCCACAGATTGCTTTTCCC
  AGAGGTTCCGCTTGCTGGATACGTCTCCTGGTCTCAAGTCAGAAGGTTTGGGAGCAGGTG
  ACTTGTTTCCATCTGGGGTTTTAGTTAGCCTTTCATTGATGCGGCTAGAAACCCCTACCT
  CAAGCCACAGTTTCCTTATTTGTGTGCCTGCTGCAGTGGGGGATGATACACATTCCCTTT
  CTCCACTACTTTGATGTTGCGTACACCTTACCAGAACAAGAAATCCGAGCTGCCGCTTTG
  AAGTTTGCGCTTTTTGTCCTCATTGCCTGGTTACTGCTCAGTCTGCGCCAATCTTGAAAC
  ATTGCTGTTGAAGGGCGATATGAACCATGCTTTTTTGCCAAATTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004342
• Insert Size: 2510 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004342.5, NP_004333.1
• RefSeq Size: 4468 bp
• RefSeq ORF: 1617 bp
• Locus ID: 800
• Cytogenetics: 7q33
• Domains: Caldesmon
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. It is mainly expressed in non-muscle tissues or cells. The resulting isoform (2, also known as WI-38 l-CaD II) lacks an internal region, compared to isoform 1.