AP2S1 (NM_004069) Human Untagged Clone

CAT#: SC109068

AP2S1 (untagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2S1
• Synonyms: AP17; CLAPS2; FBH3; FBHOk; HHC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109068 sequence for NM_004069 edited (data generated by NextGen Sequencing)
  ATGATCCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGGCCAAGTGGTAC
  ATGCAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCATGCCGTGGTCACC
  GTCCGAGACGCCAAACACACCAACTTTGTGGAGTTCCGGAACTTTAAGATCATTTACCGC
  CGCTATGCTGGCCTCTACTTCTGCATCTGTGTGGATGTCAATGACAACAACCTGGCTTAC
  CTGGAGGCCATTCACAACTTCGTGGAGGTCTTAAACGAATATTTCCACAATGTCTGTGAA
  CTGGACCTGGTGTTCAACTTCTACAAGGTTTACACGGTCGTGGACGAGATGTTCCTGGCT
  GGCGAAATCCGAGAGACCAGCCAGACGAAGGTGCTGAAACAGCTGCTGATGCTACAGTCC
  CTGGAGTGA
  
  Clone variation with respect to NM_004069.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004069 unedited
  TGTTCGAATTTTGTNATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCTACA
  ACTGCACCCTGAGCCGGAGCTGCCCAGTCGCCGCGGGACCGGGGCCGCTGGGGTCTGGAC
  GGGGGTCGCCATGATCCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGGC
  CAAGTGGTACATGCAGTTTGATGATGATGACAAACAGAAGCTGATCGAGGAGGTGCATGC
  CGTGGTCACCGTCCGAGACGCCAAACACACCAACTTTGTGGAGTTCCGGAACTTTAAGAT
  CATTTACCGCCGCTATGCTGGCCTCTACTTCTGCATCTGTGTGGATGTCAATGACAACAA
  CCTGGCTTACCTGGAGGCCATTCACAACTTCGTGGAGGTCTTAAACGAATATTTCCACAA
  TGTCTGTGAACTGGACCTGGTGTTCAACTTCTACAAGGTTTACACGGTCGTGGACGAGAT
  GTTCCTGGCTGGCGAAATCCGAGAGACCAGCCAGACGAAGGTGCTGAAACAGCTGCTGAT
  GCTACAGTCCCTGGAGTGAGGGCAGGCGAGCCCCACCCCGGCCCCGGCCCCTCCTGGACT
  CACCTGCTCGCTTCCCCTTCCCAGGCCCGTGGCCAACCCAGCAGTCCTTCTCCTCAGCTG
  CCTAGGAGGAAGGGACCCAGCTGGGTCTGGGCCACAAAGGAGGAGACTGCACCCCACTGC
  CTCTGGGCCCTGGCTGTGGGCAGAAGCCACCGTGTGTGCTCCGAGTAACCGTGCCGTTGT
  CGCGTGATGCCCTAACCGTCTGTGCGTGAAGTCCCCAATAAACTGNGGTCCTGCCTGGCA
  AAAAAAAAAAAAAAACTTGATTTACATGTGGCCGCGGTATAACTGTTTCTGAAAAACCCG
  GGGGGATCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004069 unedited
  TGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCCAGGCAGGACCAC
  AGGTTTATTGGGGACTCCACGCACAGACGCTTATGGCATCACACGACAACGGCACGGTTA
  CTCGGGACACACACGGTGGCCTCTGCCCACAGCCAGGGCCCAGAGGCAGTGGGGTGCAGT
  CTCCTCCCTTGTGGCCCAGACCCAGCTGGGTCCCTTCCTCCTAGGCAGCTGAGGGAAGGA
  CTGCTGGGTTGGCCACGGGCCTGGGAAGGGGAAGCGAGCAGGCGAGTCCAGGAGGGGCCG
  GGGCCGGGGTGGGGCTCGCCTGCCCTCACTCCAGGGACTGTAGCATCAGCAGCTGTTTCA
  GCACCTTCGTCTGGCTGGTCTCTCGGATTTCGCCAGCCAGGAACATCTCGTCCACGACCG
  TGTAAACCTTGTAGAAGTTGAACACCAGGTCCAGTTCACAGACATTGTGGAAATATTCGT
  TTAAGACCTCCACGAAGTTGTGAATGGCCTCCAGGTAAGCCAGGTTGTTGTCATTGACAT
  CCACACAGATGCAGAAGTAGAGGCCAGCATAGCGGCGGTAAATGATCTTAAAGTTCCGGA
  ACTCCACAAAGTTGGTGTGTTTGGCGTCTCGGACGGTGACCACGGCATGCACCTCCTCGA
  TCAGCTTCTGTTTCTCAAATCATCAAACTGCATGTACCACTTGGCCAGGCGCGTCTTGCC
  TGCCCCGTTCTGGATGAGGATAAAGCGATCATGGNGACCCCCGTCCAGACCCAGCGGNNC
  CCGTCCCGGGNGACTGGGCAGCNTCGCTTCAGGGTGCANTTGTAGCCTCGTGCCGATTCG
  CGGGGCGCCTATAATGAGTCGTATACAAAATCTGACGGGTCACTAACGAGCTTGCTATAT
  TAGACTNCCACGTACAGCCTACGGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004069
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004069.3, NP_004060.2
• RefSeq Size: 890 bp
• RefSeq ORF: 429 bp
• Locus ID: 1175
• Cytogenetics: 19q13.32
• Domains: Clat_adaptor_s
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (AP17) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 3. It encodes isoform AP17, which has a shorter and distinct N-terminus, compared to isoform 3.