AP2S1 (NM_021575) Human Untagged Clone

CAT#: SC109070

AP2S1 (untagged)-Human adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2S1
• Synonyms: AP17; CLAPS2; FBH3; FBHOk; HHC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_021575 edited
  ATGATCCGCTTTATCCTCATCCAGAACCGGGCAGGCAAGACGCGCCTGGCCAAGTGGTAC
  ATGCAGTTTGATGATGATGAGAAACAGAAGCTGATCGAGGAGGTGCATGCCGTGGTCACC
  GTCCGAGACGCCAAACACACCAACTTTGTGGAGTTCCGGAACTTTAAGATCATTTACCGC
  CGCTATGCTGGCCTCTACTTCTGCATCTGTGTGGATGTCAATGACAACAACCTGGCTTAC
  CTGGAGGCCATTCACAACTTCGTGGAGGTCTTAAACGAATATTTCCACAATGTCTGTGAA
  CTGGACCTGGTGTTCAACTTCTACAAGGTTTACACGGTCGTGGACGAGATGTTCCTGGCT
  GGCGAAATCCGAGAGACCAGCCAGACGAAGGTGCTGAAACAGCTGCTGATGCTACAGTCC
  CTGGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021575 unedited
  GGGGTTTAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGACC
  GGGGCCGCTGGGGTCTGGACGGGGGTCGCCATGATCCGCTTTATCCTCATCCAGAACCGG
  GCAGGCAAGACGCGCCTGGCCAAGTGGTACATGCAGTTTGATGATGATGAGAAACAGAAG
  CTGATCGAGGAGGTGCATGCCGTGGTCACCGTCCGAGACGCCAAACACACCAACTTTGTG
  GAGTTCCGGAACTTTAAGATCATTTACCGCCGCTATGCTGGCCTCTACTTCTGCATCTGT
  GTGGATGTCAATGACAACAACCTGGCTTACCTGGAGGCCATTCACAACTTCGTGGAGGTC
  TTAAACGAATATTTCCACAATGTCTGTGAACTGGACCTGGTGTTCAACTTCTACAAGGTT
  TACACGGTCGTGGACGAGATGTTCCTGGCTGGCGAAATCCGAGAGACCAGCCAGACGAAG
  GTGCTGAAACAGCTGCTGATGCTACAGTCCCTGGAGTGAGGGCAGGCGAGCCCCACCCCG
  GCCCCGGCCCCTCCTGGACTCGCCTGCTCGCTTCCCCTTCCCAGGCCCGTGGCCAACCCA
  GCAGTCCTTCCCTCAGCTGCCTAGGAGGAAGGGACCCAGCTGGGTCTGGGCCACAAGGGA
  GGAGACTGCACCCCACTGCCTCTGGGCCCTGGCTGTGGGCAGAGGCCACCGTGTGTGTCC
  CGATAACCGTGCCGTTGTCGTGTGATGCCATAAGCGTCTGTGCGTGGAGTTCCCCATAAA
  CCTGTGGTCCTGCNNNNAANNNNNNNNNNNCNNNNNTTTTTCNNNCTTTTTCNTNNCNTT
  TTTTNNTTTTNTTNTTTTTTTTTTNNTTTTTTTTTTTTTTCTCTTTTTTTTTTTCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021575
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021575.2, NP_067586.1
• RefSeq Size: 776 bp
• RefSeq ORF: 315 bp
• Locus ID: 1175
• Cytogenetics: 19q13.32
• Domains: Clat_adaptor_s
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (AP17delta) contains an alternate 5' terminal exon, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the coding region, compared to variant 3. It encodes isoform AP17delta, which is shorter and has a distinct N-terminus, compared to isoform 3.