COL8A1 (NM_020351) Human Untagged Clone

CAT#: SC109090

COL8A1 (untagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL8A1
• Synonyms: C3orf7
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020351, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCCTGAGTTCCATCAGGC
  TCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAAATTCCTCCTCAGATGCCACCACA
  AATTCCACAATACCAGCCCCTGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATG
  GGCAAGGAGATGCCCCACTTGCAGTATGGCAAAGAGTATCCACACCTACCCCAATATATGAAGGAAATTC
  AACCGGCGCCAAGAATGGGCAAGGAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTAGCCAGTTTACG
  AGGGGAACAAGGTCCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGTCAT
  GGGATACCTGGAATTAAAGGAAAACCAGGGCCACAGGGATATCCAGGAGTTGGAAAGCCAGGTATGCCTG
  GAATGCCAGGGAAGCCAGGAGCCATGGGCATGCCTGGGGCAAAAGGAGAAATTGGACAGAAAGGGGAAAT
  TGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCTCATGGACTTCCTGGCATTGGGAAGCCA
  GGTGGGCCAGGGTTACCAGGGCAACCAGGACCAAAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTC
  AAGGCCTTCGGGGTCCTAAAGGAGACAAGGGCTTCGGGATGCCAGGTGCGCCAGGTGTAAAGGGGCCTCC
  AGGGATGCACGGCCCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCAAACCAGGAGTGACAGGCTTCCCT
  GGGCCCCAGGGCCCCCTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATTGGGGTAC
  CGGGGGTTCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAGGCCAGGATGGGATCCCAGGCCAGCC
  AGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCAGGGCTACCAGGACCCCCAGGCCTTCCAGGGATT
  GGGAAACCAGGCTTCCCAGGACCCAAAGGTGACCGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGACCAA
  GAGGGGAGAAAGGACCAATAGGTGCCCCAGGAATAGGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAAT
  CCCAGGTCCTATGGGCCCTCCAGGTGCTATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTGTAGGG
  CCACAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAGCCAGGTTTCCTTG
  GTGAAGTAGGGCCTCCTGGCATGAGGGGTTTGCCAGGTCCCATAGGGCCCAAGGGGGAAGCTGGGCAAAA
  AGGTGTACCAGGACTCCCTGGTGTTCCAGGGCTTCTCGGACCTAAGGGAGAGCCAGGAATCCCAGGGGAT
  CAGGGTTTACAGGGCCCCCCAGGTATCCCAGGGATTGGGGGCCCTAGTGGCCCCATTGGACCACCTGGGA
  TTCCAGGCCCCAAAGGGGAGCCGGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGT
  GGCAGGACTTCATGGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCCTCAAGGCCAGCCTGGCCTTCCAGGA
  CCCCCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTGTGATGCCCCCTACACCACCACCCCAGGGAGAGT
  ATCTGCCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTACGGGGCTAAGAAAGGCAA
  GAATGGAGGGCCAGCCTATGAGATGCCTGCATTTACCGCCGAGCTAACCGCACCTTTCCCACCGGTGGGG
  GCCCCAGTGAAGTTTAACAAACTGCTGTATAACGGCAGACAGAACTACAACCCGCAGACAGGCATCTTCA
  CCTGTGAGGTCCCTGGTGTCTACTACTTTGCATACCACGTTCACTGCAAGGGGGGCAACGTGTGGGTTGC
  TCTATTCAAGAACAACGAGCCCGTGATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCA
  TCTGGGAGTGCAGTGCTGCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGCCCTCAGAACAGGCTG
  CAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATATTTATTGTATCCCATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020351 unedited
  NTTATAACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC
  CGCGAATTCGGCACGAGGCGATCCTCTCCGTGGGAGCCAGCGAGCCTCTCTCCCTGATCT
  TACGTGCTCAAGAAGCTGTTGTGAAGGCAGAGCAGCATCTGCTGAAGAGACAGAAACCAG
  CCCCAGAGGTGTCACAGGAAGGCACCAGCAAGGACATTGGTCTTTGATTTGATTCAGCAG
  TCCTGTCAAGTATAAATGTGATGGCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGC
  TGCTTACCATTTCCCTGAGTTCCATCAGGCTCATTCAGGCTGGTGCCTACTATGGGATCA
  AGCCGCTGCCACCTCAAATTCCTCCTCAGATGCCACCACAAATTCCACAATACCAGCCCC
  TGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATGGGCAAGGAGA
  TGCCCCACTTGCAGTATGGCAAAGAGTATCCACACCTACCCCAATATATGAAGGAAATTC
  AACCGGCGCCAAGAATGGGCAAGGNAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTA
  GCCAGTTNACGAGGGNAAACAAGTCCCCCGTGGNAGAGCCTGGCCCAAGAGGACACCTGG
  CCNNCCGGGGNTTGGCAGGTCATTGGGATCCCTGGAAATTAAAAGGAAAACCAGGGCCAC
  AGGGATTNCCCGGAGGTTGGAAAGCCCAGTATGCCCTGAAATGCCAGGAAGCCAGGAGCA
  TGGGCATGCCTGGGGCAAAGGAAAAATTGGACAGAGGGGGGAATTTGGCCTTAGGGGGAT
  CCAGAACCCAAGGACT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_020351
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020351.2, NP_065084.2
• RefSeq Size: 2614 bp
• RefSeq ORF: 2235 bp
• Locus ID: 1295
• Cytogenetics: 3q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.