CPT1B (NM_004377) Human Untagged Clone

CAT#: SC109096

CPT1B (untagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPT1B
• Synonyms: CPT1-M; CPT1M; CPTI; CPTI-M; M-CPT1; MCCPT1; MCPT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004377 edited
  ATGGCGGAAGCTCACCAGGCCGTGGCCTTCCAGTTCACGGTGACCCCAGACGGGGTCGAC
  TTCCGGCTCAGTCGGGAGGCCCTGAAACACGTCTACCTGTCTGGGATCAACTCCTGGAAG
  AAACGCCTGATCCGCATCAAGAATGGCATCCTCAGGGGCGTGTACCCTGGCAGCCCCACC
  AGCTGGCTGGTCGTCATCATGGCAACAGTGGGTTCCTCCTTCTGCAACGTGGACATCTCC
  TTGGGGCTGGTCAGTTGCATCCAGAGATGCCTCCCTCAGGGGTGTGGCCCCTACCAGACC
  CCGCAGACCCGGGCACTTCTCAGCATGGCCATCTTCTCCACGGGCGTCTGGGTGACGGGC
  ATCTTCTTCTTCCGCCAAACCCTGAAGCTGCTTCTCTGCTACCATGGGTGGATGTTTGAG
  ATGCATGGCAAGACCAGCAACTTGACCAGGATCTGGGCTATGTGTATCCGCCTTCTATCC
  AGCCGGCACCCTATGCTCTACAGCTTCCAGACATCTCTGCCCAAGCTTCCTGTGCCCAGG
  GTGTCAGCCACAATTCAGCGGTACCTAGAGTCTGTGCGCCCCTTGTTGGATGATGAGGAA
  TATTACCGCATGGAGTTGCTGGCCAAAGAATTCCAGGACAAGACTGCCCCCAGGCTGCAG
  AAATACCTGGTGCTCAAGTCATGGTGGGCAAGTAACTATGTGAGTGACTGGTGGGAAGAG
  TACATCTACCTTCGAGGCAGGAGCCCTCTCATGGTGAACAGCAACTATTATGTCATGGAC
  CTTGTGCTCATCAAGAATACAGACGTGCAGGCAGCCCGCCTGGGAAACATCATCCACGCC
  ATGATCATGTATCGCCGTAAACTGGACCGTGAAGAAATCAAGCCTGTGATGGCACTGGGC
  ATAGTGCCTATGTGCTCCTACCAGATGGAGAGGATGTTCAACACCACTCGGATCCCGGGC
  AAGGACACAGATGTGCTACAGCACCTCTCAGACAGCCGGCACGTGGCTGTCTACCACAAG
  GGACGCTTCTTCAAGCTGTGGCTCTATGAGGGCGCCCGTCTGCTCAAGCCTCAGGATCTG
  GAGATGCAGTTCCAGAGGATCCTGGACGACCCCTCCCCACCTCAGCCTGGGGAGGAGAAG
  CTGGCAGCCCTCACTGCAGGAGGAAGGGTGGAGTGGGCGCAGGCACGCCAGGCCTTCTTT
  AGCTCTGGAAAGAATAAGGCTGCCTTGGAGGCCATCGAGCGTGCCGCTTTCTTCGTGGCC
  CTGGATGAGGAATCCTACTCCTATGACCCCGAAGATGAGGCCAGCCTCAGCCTCTATGGC
  AAGGCCCTGCTACATGGCAACTGCTACAACAGGTGGTTTGACAAATCCTTCACTCTCATT
  TCCTTCAAGAATGGCCAGTTGGGTCTCAATGCAGAGCATGCGTGGGCAGATGCTCCCATC
  ATTGGGCACCTCTGGGAGTTTGTCCTGGGCACAGACAGCTTCCACCTGGGCTACACGGAG
  ACCGGGCACTGCCTGGGCAAACCGAACCCTGCGCTCGCACCTCCTACACGGCTGCAGTGG
  GACATTCCAAAACAGTGCCAGGCGGTCATCAAGAGTTCCTACCAGGTGGCCAAGGCGTTG
  GCAGACGACGTGGAGTTGTACTGCTTCCAGTTCCTGCCCTTTGGCAAAGGCCTCATCAAG
  AAGTGCCGGACCAGCCCTGATGCCTTTGTGCAGATCGCGCTGCAGCTGGCTCACTTCCGG
  GACAGGGGTAAGTTCTGCCTGACCTATGAGGCCTCAATGACCAGAATGTTCCGGGAGGGA
  CGGACTGAGACTGTGCGTTCCTGTACCAGCGAGTCCACAGCCTTTGTGCAGGCCATGATG
  GAGGGGTCCCACACAAAAGCAGACCTGCGAGATCTCTTCCAGAAGGCTGCTAAGAAGCAC
  CAGAATATGTACCGCCTGGCCATGACCGGGGCAGGGATCGACAGGCACCTCTTCTGCCTT
  TACTTGGTCTCCAAGTACCTAGGAGTCAGCTCTCCTTTCCTTGCTGAGGTGCTCTCGGAA
  CCCTGGCGTCTCTCCACCAGCCAGATCCCCCAATCCCAGATCCGCATGTTCGACCCAGAG
  CAGCACCCCAATCACCTGGGCGCTGGAGGTGGCTTTGGCCCTGTAGCAGATGATGGCTAT
  GGAGTTTCCTACATGATTGCAGGCGAGAACACGATCTTCTTCCACATCTCCAGCAAGTTC
  TCAAGCTCAGAGACGAACGCCCAGCGCTTTGGAAACCACATCCGCAAAGCCCTGCTGGAC
  ATTGCTGATCTTTTCCAAGTTCCCAAGGCCTACAGCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004377 unedited
  ACTTGGATTTGTAACCGACTTTATATAGGCGGCCGCGCAATCGGCACGAGGAGTGAACCC
  GAGCTGTGCCGACCAACCCCCAGGAGGCGGAAGCTCACCAGGCCGTGGCCTTCCAGTTCA
  CGGTGACCCCAGACGGGGTCGACTTCCGGCTCAGTCGGGAGGCCCTGAAACACGTCTACC
  TGTCTGGGATCAACTCCTGGAAGAAACGCCTGATCCGCATCAAGAATGGCATCCTCAGGG
  GCGTGTACCCTGGCAGCCCCACCAGCTGGCTGGTCGTCATCATGGCAACAGTGGGTTCCT
  CCTTCTGCAACGTGGACATCTCCTTGGGGCTGGTCAGTTGCATCCAGAGATGCCTCCCTC
  AGGGGTGTGGCCCCTACCAGACCCCGCAGACCCGGGCACTTCTCAGCATGGCCATCTTCT
  CCACGGGCGTCTGGGTGACGGGCATCTTCTTCTTCCGCCAAACCCTGAAGCTGCTTCTCT
  GCTACCATGGGTGGATGTTTGAGATGCATGGCAAGACCAGCAACTTGACCAGGATCTGGG
  CTATGTGTATCCGCCTTCTATCCAGCCGGCACCCTATGCTCTACAGCTTCCAGACATCTC
  TGCCCAAGCTTCCTGTGCCCAGGGTGTCAGCCACAATTCAGCGGTACCTAGAGTCTGTGC
  GCCCCTTGTTGGATGATGAGGAATATTACCGCATGGAGTTGCTGGCCAAAGAATTCCAGG
  ACAAGACTGCCCCCAGGCTGCAGAAATACCTGGTGCTCAAGTCATGGTGGGCAAGTAACT
  ATGTGAGTGACTGGTGGGAAGAGTACATCTACCTTCGAGGCAGGAGCCCTCTCATGGTGA
  AAACAGCACTATATGTCATGGACCTTGTGCTCATCAAGATACAGACGTGCANGCAGCCCG
  CCTGGGGAACATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004377 unedited
  TGAACCGCAGGCACGCAATCTATAGTCGAGNNCTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTCGGAAAAAAAAAACCCAGCCTCTATTTTTTTTATT
  TTTTTTCAAAAAAAAAAAAAATCAAAATTCCCTTTCTGGTCCAACCCCACCTCCACCACT
  TGGGCCCTTAGGGAGCAACTTTTGCCCAGGGGGGACCTGGGGCCGGAGCTGCCCTTGGAC
  CCTGGGCCCCTGGGCACGGCCCCCCCTTATGCCTGGGAGCTGCCACAGGCCCCTTCCTCC
  CCAGGGGGGGGACAAAGGGGCAACTGGCTTTTTTCCAACCTTCAACTGGGGGCCTGGGGA
  CCTTGGAAAAAATCACCAATGCCCAACGGGGCTTTGGGGATGGGGGTTCCAAAGCCCCGG
  GGGGGCCGCCCTTGACCTTGAAAACTGGCCGGACACTCCGGAAAAAAATCGGGTTCTCCC
  CCCCCCCCCTGGCGGAAACCCCCCTGCCTTCTTTTGTTCTCGGGCCCAAGCCCCCCTCCC
  CCCCCCCTCGGGCCGGCGGGGCCGCCTTGGGGTCTAACCCCGCCGCTCTCGGATTTGCGG
  CGACCTGCGCTGTTGCTATCCCCCCACCCCCTCCGATATCCCCCCATCTGGGGTCCGGCG
  CCCTCCTCCCCTGCCCTCCTCGTCCGCCTGCCCCTACTCACATTGATTTGTTCCTAGTTC
  CTCGTCCCCCCGTCCGGCCGCCCCGCGGGATGCACTAGCGATTTTGTTACTATGCGCTTT
  CTCCCCTTTTCGGCGCTGGCCTCCCCCAGCTATAGCCCTCACGCGCCCGCGCGCTTCCCC
  TCCCGCTCTTCTCTTTCCTCTCCTCCCCTTCTCACTCTTTTTCCCTCCCCCCCCTTCGCT
  CTCCCCTGTTCTTCTCCCACTCCCCCCTGTCTACTTCTTCTGCCTCCCCACCACTACAAC
  CCGCCCTCCCCCACTCCACTACATAACACCACTTACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004377
• Insert Size: 2710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004377.2, NP_004368.1
• RefSeq Size: 2879 bp
• RefSeq ORF: 2319 bp
• Locus ID: 1375
• Cytogenetics: 22q13.33
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
• Gene Summary: 'The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 3, 6, and 8 all encode isoform a.