CRAT (NM_000755) Human Untagged Clone

CAT#: SC109099

CRAT (untagged)-Human carnitine O-acetyltransferase (CRAT), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRAT
• Synonyms: CAT; CAT1; NBIA8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000755 edited
  ATGTTAGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCC
  TTGATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCACGGCTGCCCGTG
  CCCCCTCTCCAGCAGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAG
  GAGGAGTGGGCCCACACCAAGCAGCTGGTGGATGAGTTTCAGGCCTCAGGAGGTGTAGGG
  GAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGACGGAGAACTGGCTGTCTGAG
  TGGTGGCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTACTCGAGCCCA
  GGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCCGATTTGCTGCC
  AAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTG
  GAGTACCTGGGGGGGAAGCCACTGTGCATGAACCAGTACTATCAGATCTTGTCCTCCTGC
  CGAGTGCCGGGCCCCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCC
  ACGCACATCACCGTGGTACACAACTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGAC
  GGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGGAGAAGATCTGGAACTCATCC
  CTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAACTCCTGGGCC
  AAGGCATACAACACCCTCATCAAAGACAAGGTGAACCGGGATTCCGTGCGCTCCATCCAG
  AAGAGCATCTTCACCGTGTGCCTAGATGCAACCATGCCCAGGGTCTCAGAAGACGTGTAC
  CGCAGCCACGTGGCAGGCCAGATGCTGCATGGGGGCGGCAGCAGGCTCAACAGCGGCAAC
  CGCTGGTTCGACAAGACGCTGCAGTTCATCGTGGCAAAAGATGGCTCCTGTGGGCTTGTG
  TACGAGCATGCTGCAGCGGAGGGGCCCCCTATTGTCACCCTTCTGGACTATGTCATCGAG
  TACACGAAGAAACCCGAGCTTGTGCGGTCTCCCATGGTGCCCCTGCCCATGCCCAAGAAG
  CTGCGGTTCAACATCACCCCCGAGATCAAGAGCGACATCGAGAAGGCCAAGCAGAACCTC
  AGCATCATGATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTTGGAAAAGAC
  TTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATGGCTTTGCAGCTGGCC
  TACTACAGGATCTACGGACAGGCATGTGCCACCTATGAAAGTGCCTCCCTGCGCATGTTT
  CACCTGGGCCGCACCGACACCATCCGCTCGGCTTCCATGGACTCACTCACCTTTGTCAAG
  GCCATGGATGACTCCAGCGTCACGGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTG
  CAGGCCCACCGAGGCTACACCGACCGGGCCATCCGCGGGGAGGCCTTTGATCGACACCTG
  CTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGCCCGACATCTTCATGGAC
  ACCTCCTACGCCATCGCCATGCACTTCCACCTCTCCACCAGCCAGGTCCCTGCCAAGACA
  GACTGTGTCATGTTCTTCGGGCCCGTGGTCCCCGACGGCTACGGTGTCTGCTATAACCCC
  ATGGAGGCCCACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCC
  GCCCGCCTGGCGCATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCAGAGC
  CACCCCCGGGCCAAGCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000755 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCGAAGAGTTAGCCT
  TCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGG
  CTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCACGGCTGCCCGTGCCCCCTCTCC
  AGCAGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGG
  CCCACACCAAGCAGCTGGTGGATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGC
  AGAAGGGGCTGGAGCGTCGGGCCAGGAAGACGGAGAACTGGCTGTCTGAGTGGTGGCTCA
  AGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTACTCGAGCCCAGGCGTGATGC
  TACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCCGATTTGCTGCCAAACTCATTG
  AGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGG
  GGGGGAAGCCACTGTGCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGG
  GCCCCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCACATCA
  CCGTGGTACACAACTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCC
  TCACTGCGGATCAGATCTTTGTGCAGCTGGAGAAGATCTGGAACTCATCCCTACAGACCA
  ACAAGGAGCCTGTGGGCATNCTCACCTNCAACCACCGGAACTNCTGGGCCAGGCATACAA
  CACCCTCATCAAAGACAGGTGAACCGGNNATNCGTGCGCTCCATNCAGAAGAGCATCTTA
  CCGTGTGCCTAGATGCAACATGCCCANGGTCTCANAAGACGTGTACCGCACCCACGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000755 unedited
  GTGGGCGGGGCGGTNAAANAATATTTATATCACCAACAGCCNCTCACCACAATACCCCAC
  CNTTGTTGNNACCTTCCACATTAATATCATACAGCAGGGGCGGGGCGCAGGTAACCAAGC
  TGAGTGGAACCAGAGCGGCTCGCTAGCAATGTTATCCACAGGAGCACAGCCGCAACGGAG
  GTGAAACCTCACACCCTGGCCCCTCGGCCCCAGACAATCCTGTCTCCAGGTCCTGGGAGT
  CCGGGAAGCACGGCACACCATGGTGGCCCGCCCATGGAGTTGGCAGGGAGTGGCGGGCGC
  TGAGCTGGTGAGACAAAGAGCTCTTGCCAGTCTCCTGCTCTGGAGGGCTGGTTCCCTTCC
  CCAGAGGAGGCACCGGTGGACGACGTGCCATGGGCCCGGGTCTAACGTGCTGTTTAGCCT
  CTGCACTCTACCCCACGTTGGTCCCTGACAGGTGCTGGGATGACCCACGGTAGGCACTTG
  CCTGGGGCCTGAATGCCCTATGGATGCCGCGGCTCGTGGCTTCACTATTTCTGGTCGGAC
  CATTGCTTATGGAACCAAGGTACAGCGTAACTATCGGTCTCTGCTTTGATGGATGGATCA
  TTTAACTGTGTTGCTGTCCGTCGCTATTCCATGTCATGTTCCTACTGTCTTTATTTTCCT
  CACCTCGGGCCGCCCCTGGATTTTGGTACTTCTGCTCTGTATCACATTTTCTTCTTATTT
  CTCTTGTTTTCTCTCTTCCTTCCTCCATCTCTTCATTGCTTTTTTAATTTTTTAATTTAT
  CTATCATTTTCTTCATTTCTCTATATTCTTATCCCCTTTCCTCCCAATAATCTCCCTCTT
  TCTCCTTCCACACCTTCTTCCCCCTCGTCTCCTCCAGGTTCCCTTGTCTCTCCTATCACA
  GACAAAACACCCCCCTATTCATGCGTTCCCTCCCCCCCCATATCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000755
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000755.2, NP_000746.2
• RefSeq Size: 2804 bp
• RefSeq ORF: 1881 bp
• Locus ID: 1384
• Cytogenetics: 9q34.11
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016]'
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.