CSHL1 (NM_022580) Human Untagged Clone

CAT#: SC109124

CSHL1 (untagged)-Human chorionic somatomammotropin hormone-like 1 (CSHL1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSHL1
• Synonyms: CS-5; CSHP1; CSL; GHB4; hCS-L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_022580 edited
  ATGGAGGAAACGCAGCAGAAATCCAACTTAGAGCTGCTCCACATCTCCCTGCTGCTCATC
  GAGTCGCGGCTGGAGCCCGTGCGGTTCCTCAGGAGTACCTTCACCAACAACCTGGTGTAT
  GACACCTCGGACAGTGATGAATATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAATG
  CTGATGGGGAGGCTGGAAGACGGCAGCCACCTGACTGGGCAGACCCTCAAGCAGACCTAC
  AGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTC
  CACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGC
  TCTGTGGAGGGCAGCTGTGGCTTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022580 unedited
  NNNCCAGTTCATTATTGTATACNACTCATATAGGCGGCCGCGNAATTCGCACCGCGCACC
  ACTCAGGTCCTGTGGNACAGCTCACCTAGCGGCAATGGCTGCAGACTCTATTCCGACATC
  CTCCAACATGGAGGAAACGCAGCAGAAATCCAACTTAGAGCTGCTCCACATCTCCCTGCT
  GCTCATCGAGTCGCGGCTGGAGCCCGTGCGGTTCCTCAGGAGTACCTTCACCAACAACCT
  GGTGTATGACACCTCGGACAGTGATGAATATCACCTCCTAAAGGACCTAGAGGAAGGCAT
  CCAAATGCTGATGGGGAGGCTGGAAGACGGCAGCCACCTGACTGGGCAGACCCTCAAGCA
  GACCTACAGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCTCAAGAACTACGG
  GCTGCTCCACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCA
  GTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGGGCCCGCGTGGCATCCTGTGACCCC
  TCCCCAGTGCCTCTCCTGGCCCTGAAGGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAA
  TAAAATTAAGTTGTATTGTTTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCG
  CGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCATGCC
  TCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAGT
  TGCATCATTTTGTCTGACTAGGTGTCCTTCTTATATATGTGGGGGGGGGGGGGGGTGNTN
  NTNNGGNNNNGGGGGNNNNTTNGGGGNNNNNNNNNTGTGGGGGCTGGGGGGGTTCTTTGG
  GGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022580 unedited
  TTTAAGTTCCTTTAGNACCGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTAA
  ACAATACAACTTAATTTTATTAGGACAAGGCTGGTGGGCACTGGAGTGGCACCTTCAGGG
  CCAGGAGAGGCACTGGGGAGGGGTCACAGGATGCCACGCGGGCCCCTAGAAGCCACAGCT
  GCCCTCCACAGAGCGGCACTGCACCATGCGCAGGAATGTCTCGACCTTGTCCATGTCCTT
  CCTGAAGCAGTGGAGCAGCCCGTAGTTCTTGAGCAGTGCGTCATGGTTGTGCGAGTTTGT
  GTCAAACTTGCTGTAGGTCTGCTTGAGGGTCTGCCCAGTCAGGTGGCTGCCGTCTTCCAG
  CCTCCCCATCAGCATTTGGATGCCTTCCTCTAGGTCCTTTAGGAGGTGATATTCATCACT
  GTCCGAGGTGTCATACACCAGGTTGTTGGTGAAGGTACTCCTGAGGAACCGCACGGGCTC
  CAGCCGCGACTCGATGAGCAGCAGGGAGATGTGGAGCAGCTCTAAGTTGGATTTCTGCTG
  CGTTTCCTCCATGTTGGAGGATGTCGGAATAGAGTCTGCAGCCATTGCCGCTAGGTGAGC
  TGTCCACAGGACCCTGAGTGGTGCGCTGGTGCCGAATTCGCGGCCGCCCTATAGTGAGTC
  GTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTA
  CACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGGAAGTCCC
  GTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACCTGGGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022580
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022580.1, NP_072102.1
• RefSeq Size: 588 bp
• RefSeq ORF: 420 bp
• Locus ID: 1444
• Cytogenetics: 17q23.3
• Domains: hormone
• Protein Families: Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4), also known as CS-1-3L-4-5, lacks an in-frame segment of the coding region, compared to variant 1. The resulting isoform (4) has a distinct N-terminus, which does not contain a signal peptide, compared to isoform 1.