RNA Helicase A (DHX9) (NM_001357) Human Untagged Clone

CAT#: SC109149

DHX9 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 9 (DHX9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHX9
• Synonyms: DDX9; LKP; NDH2; NDHII; RHA
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001357, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGACGTTAAAAATTTTCTGTATGCCTGGTGTGGCAAAAGGAAGATGACCCCATCCTATGAAATTA
  GAGCAGTGGGGAACAAAAACAGGCAGAAATTCATGTGTGAGGTTCAGGTGGAAGGTTATAATTACACTGG
  CATGGGAAATTCCACCAATAAAAAAGATGCACAAAGCAATGCTGCCAGAGACTTTGTTAACTATTTGGTT
  CGAATAAATGAAATAAAGAGTGAAGAAGTTCCAGCTTTTGGGGTAGCATCTCCGCCCCCACTTACTGATA
  CTCCTGACACTACAGCAAATGCTGAAGGAGATTTACCAACAACCATGGGAGGACCTCTTCCTCCACATCT
  GGCTCTCAAAGCAGAAAATAATTCTGAGGTAGGGGCCTCTGGCTATGGTGTTCCTGGGCCCACCTGGGAC
  CGAGGAGCCAACTTGAAGGATTACTACTCAAGAAAGGAAGAACAAGAAGTGCAAGCGACTCTAGAATCAG
  AAGAAGTGGATTTAAATGCTGGGCTTCATGGAAACTGGACCTTGGAAAATGCTAAAGCTCGTCTAAACCA
  ATATTTTCAGAAAGAAAAGATCCAAGGAGAATATAAGTACACCCAAGTGGGTCCTGATCACAACAGGAGC
  TTTATTGCAGAAATGACCATTTATATCAAGCAGCTGGGCAGAAGGATTTTTGCACGAGAACATGGATCAA
  ATAAGAAATTGGCAGCACAGTCCTGTGCCCTGTCACTTGTCAGACAACTGTACCATCTTGGAGTGGTTGA
  AGCTTACTCCGGACTTACAAAGAAGAAGGAAGGAGAGACAGTGGAGCCTTACAAAGTAAACCTCTCTCAA
  GATTTAGAGCATCAGCTGCAAAACATCATTCAAGAGCTAAATCTTGAGATTTTGCCCCCGCCTGAAGATC
  CTTCTGTGCCAGTTGCACTCAACATTGGCAAATTGGCTCAGTTCGAACCATCTCAGCGACAAAACCAAGT
  GGGTGTGGTTCCTTGGTCACCTCCACAATCCAACTGGAATCCTTGGACTAGTAGCAACATTGATGAGGGG
  CCTCTGGCTTTTGCTACTCCAGAGCAAATAAGCATGGACCTCAAGAATGAATTGATGTACCAGTTGGAAC
  AGGATCATGATTTGCAAGCAATCTTGCAGGAGAGAGAGTTACTGCCTGTGAAGAAATTTGAAAGTGAGAT
  TCTGGAAGCAATCAGCCAAAATTCAGTTGTCATTATTAGAGGGGCTACTGGATGTGGGAAAACCACACAG
  GTTCCCCAGTTCATTCTAGATGACTTTATCCAGAATGACCGAGCAGCAGAGTGTAACATCGTAGTAACTC
  AGCCCAGAAGAATCAGTGCGGTTTCTGTGGCAGAGCGAGTTGCATTTGAAAGAGGAGAAGAGCCTGGAAA
  AAGCTGTGGCTACAGCGTTCGATTTGAGTCTATACTTCCTCGTCCTCATGCCAGTATAATGTTTTGTACT
  GTAGGTGTGCTCCTGAGAAAATTAGAAGCAGGCATTCGAGGAATCAGTCATGTAATTGTAGATGAAATAC
  ATGAAAGAGATATTAATACTGACTTCCTTTTGGTAGTACTGCGTGATGTTGTTCAGGCTTATCCTGAAGT
  TCGCATTGTTCTTATGTCTGCTACTATTGATACCAGCATGTTTTGTGAATATTTCTTCAATTGCCCCATC
  ATTGAAGTTTATGGGAGGACTTACCCAGTTCAAGAATATTTTCTGGAAGACTGCATTCAGATGACCCACT
  TTGTTCCTCCACCAAAAGACAAAAAGAAGAAGGATAAGGATGATGATGGTGGTGAGGATGATGATGCAAA
  TTGCAACTTGATCTGTGGTGATGAATATGGTCCAGAAACAAGGTTGAGCATGTCTCAATTGAACGAAAAG
  GAAACTCCTTTTGAACTCATCGAGGCTCTACTTAAGTACATTGAAACCCTTAATGTTCCTGGAGCTGTGT
  TGGTTTTTTTGCCTGGCTGGAATCTGATTTATACTATGCAGAAGCATTTGGAAATGAATCCACATTTTGG
  AAGCCATCGGTATCAGATTCTACCCCTGCATTCTCAGATTCCTCGAGAGGAACAGCGCAAAGTGTTTGAT
  CCAGTACCAGTTGGAGTAACCAAGGTTATTTTGTCCACAAATATTGCTGAAACAAGCATTACCATAAACG
  ATGTTGTTTATGTCATTGACTCCTGCAAGCAGAAAGTGAAACTCTTCACTGCTCACAACAATATGACCAA
  CTATGCTACCGTATGGGCATCAAAAACAAACCTTGAGCAACGGAAAGGGCGAGCTGGCCGAGTACGGCCT
  GGATTCTGCTTTCACCTGTGCAGCCGAGCTCGTTTTGAGAGACTTGAAACCCACATGACACCAGAGATGT
  TCCGAACACCATTGCATGAAATTGCTCTTAGCATAAAACTTCTGCGTCTAGGAGGAATTGGCCAATTTCT
  GGCCAAAGCAATTGAACCTCCCCCTTTGGATGCTGTGATTGAAGCAGAACACACTCTTAGAGAGCTTGAT
  GCATTAGATGCCAATGATGAGTTGACTCCTTTGGGACGAATCCTGGCTAAACTCCCCATTGAGCCTCGTT
  TTGGCAAAATGATGATAATGGGGTGTATTTTCTACGTGGGAGATGCTATCTGTACCATTGCTGCTGCTAC
  CTGCTTTCCAGAGCCTTTCATCAATGAAGGAAAGCGGCTGGGCTATATCCATCGAAATTTTGCTGGAAAC
  AGATTTTCTGATCACGTAGCCCTTTTATCAGTATTCCAAGCCTGGGATGATGCTAGAATGGGTGGAGAAG
  AAGCAGAGATACGTTTTTGTGAGCACAAAAGACTTAATATGGCTACACTAAGAATGACCTGGGAAGCCAA
  AGTTCAGCTCAAAGAGATTTTGATTAATTCTGGGTTTCCAGAAGATTGTTTGTTGACACAAGTGTTTACT
  AACACTGGACCAGATAATAATTTGGATGTTGTTATCTCCCTCCTGGCCTTTGGTGTGTACCCCAATGTAT
  GCTATCATAAGGAAAAGAGGAAGATTCTCACCACTGAAGGGCGTAATGCACTTATCCACAAATCATCTGT
  TAATTGTCCTTTTAGTAGCCAAGACATGAAGTACCCATCTCCCTTCTTTGTATTTGGTGAAAAGATTCGA
  ACTCGAGCCATCTCTGCTAAAGGCATGACTTTAGTCACCCCCCTGCAGTTGCTTCTCTTTGCCTCCAAGA
  AAGTCCAATCTGATGGGCAGATTGTGCTTGTAGATGACTGGATTAAACTGCAAATATCTCATGAAGCTGC
  TGCCTGTATCACTGGTCTCCGGGCAGCCATGGAGGCTTTGGTTGTTGAAGTAACCAAACAACCTGCTATC
  ATCAGCCAGTTGGACCCCGTAAATGAACGTATGCTGAACATGATCCGTCAGATCTCTAGACCCTCAGCTG
  CTGGTATCAACCTTATGATTGGCAGTACACGGTATGGAGATGGTCCACGTCCTCCCAAGATGGCCCGATA
  CGACAATGGAAGCGGATATAGAAGGGGAGGTTCTAGTTACAGTGGTGGAGGCTATGGCGGTGGCTATAGC
  AGTGGAGGCTATGGTAGCGGAGGCTATGGTGGCAGCGCCAACTCCTTTCGGGCAGGATATGGTGCAGGTG
  TTGGTGGAGGCTATAGAGGAGTTTCCCGAGGTGGCTTTAGAGGCAACTCTGGAGGAGACTACAGAGGGCC
  TAGTGGAGGCTACAGAGGATCTGGGGGATTCCAGCGAGGAGGTGGTAGGGGGGCCTATGGAACTGGCTAC
  TTTGGACAGGGAAGAGGAGGTGGCGGCTATTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001357
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001357.4, NP_001348.2
• RefSeq Size: 4543 bp
• RefSeq ORF: 3813 bp
• Locus ID: 1660
• Cytogenetics: 1q25.3
• Domains: DSRM, DEAD, helicase_C, HA2
• Gene Summary: 'This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) encodes the functional protein.