Dystrobrevin alpha (DTNA) (NM_032981) Human Untagged Clone

CAT#: SC109178

DTNA (untagged)-Human dystrobrevin, alpha (DTNA), transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DTNA
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109178 sequence for NM_032981 edited (data generated by NextGen Sequencing)
  ATGTTCCCAGATCAGCCTGAGAAGCCACTCAACTTGGCTCACATCGTGCCTCCCAGACCT
  GTAACCAGCATGAACGACACCCTGTTCTCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTT
  ATTACCAGGAGCATGCTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGCTAATTGCC
  AGGTATGCGGCAAGGCTGGCAGCAGAGTCCTCTTCGTCTCAGCCACCTCAGCAGAGAAGT
  GCTCCTGACATCTCTTTCACCATCGATGCGAATAAGCAGCAAAGGCAGCTGATTGCTGAG
  CTAGAAAACAAGAACAGAGAAATCTTACAGGAGATCCAGAGACTTCGGCTAGAGCATGAA
  CAAGCTTCTCAGCCCACGCCAGAGAAGGCACAGCAAAACCCCACCCTGCTGGCAGAACTC
  CGGCTCCTCAGACAGCGCAAAGATGAGCTGGAACAGAGAATGTCTGCTCTCCAGGAGAGC
  CGGAGAGAGCTAATGGTCCAGTTGGAGGGTCTCATGAAGCTACTAAAGGAAGAAGAACTG
  AAGCAGGGAGTAAGTTATGTCCCCTACTGCAGGTCTTAA
  
  Clone variation with respect to NM_032981.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032981 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTGGTTCTCATAGC
  AACCAGCACCAAATGAAAGAGTACACGTCATGGGTAAGGCAAGGTCCAGGCAATACTTGG
  AGTTGGATGTGTGAGTGTTGCCCAGAGTTAAAGGGAATATCAAGGATGGTGGCAGAATTA
  AAGGGCAAACTTACCTTCCATCCCAGGTCTAGTATTCAGTCCCCCTTCCTCCCACTCTCC
  ACCCTACTGCGTGCTCTTACTTATTCTGTTGGAACCCAGTGTAGCTTCCTGAGATTTAAA
  ATATAGGAAGACTCAAAACTATGTTACTTCTTGGAATATAGATAATTCAGTACTGATTTA
  AATAACAACTGAATAGCAAGGACCTTCTGGAACATAATCTTACATTCACAAAATCACCTG
  CTAAGAAGCTGACTAATGCATTAAGCAAGTCCCTGAGCTGTGCTTCCAGCCGTGAACCTT
  TGCACCCCATGTTCCCAGATCAGCCTGAGAAGCCACTCAACTTGGCTCACATCGTGCTTC
  CCAGACCTGTAACCAGCATGAACGACACCCTGTTCTCCCACTCTGTTCCCTCCTCAGGAA
  GTCCTTTTATTACCAGGAGCATGCTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGC
  TAATTGCCAGGTATGCGGCAAGGCTGGCAGCAGAGTCCTCTTCGTCTCAGCCACCTCAGC
  AGAGAAGTGCTCCTGACATCTCTTTCACCATCGATGCGAATAAGCAGCANAGGCAGCTGA
  TTGCTGAGCTAGAAAACAAGACAGAGAAATCTACAGGAGATCCAGAGACTTTCGCTAGAG
  CATGAACAAGCTTCTCAGNCCACGCCAGAGAAGCACAGCANAACCCCACCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032981 unedited
  GCTTATCTTTCTTGTTTTGACTTACANATTGATTTTACACTTTCCTTTTTTACAGACCAT
  GGGNAATGAAGGGTAAAAATGTGTCCAGCATTTTCCAAACATTCTTTGTTACACACAAAC
  ACACATAATGAATAATAGAAAAGAGCATACAGAAGAAATGAAAGATACGTTGAAATTGGA
  CAGGTTGTGGTTCTAATTGATCCATGGAAATAATGTGAAACTTTTAAATAATGCTAGTGA
  GTGGGATATATTGTCATACATAGCGTGGTACATGCACTTGAAATTAAAGCTGCTTTTATT
  TTGCTTCTATTTAATTTAATTTAATTTAATTTAATTTGTTTTTTTTTTGAGTTTGCTGCT
  CCCCCTGCTGGCTCTTGAGCCTGACCACAGGTTCAGACAGCTCCAGTTTAAGCTATAAAG
  TCACAATGCCCCTATTTCCAGAAAAAAAAAAAAGTATGTAATGACCCTCTCGTCTCCCGG
  AGCCGAAAGGCTCTGGTGCTAAAGAAAAAGCTGAGAGTTACTTTTTAAAAAGAGATTACT
  GTCAACCAACAGTAAGGATTCTGCTTTGGGGAAGAGCCAAAAAGATGGGACGTGAATAGT
  CAGTTATGGAGTACAGTCTTTCTCTCACATAATNGAGCACATTCTGGTGAGCCATGGGGA
  ACAATGCTCAATAATTGCGTGATGAAAAGGGATTTAATTGCCTGATTTTTTTTAAAAGAA
  GTTTCACAAGTTTTCAGTAAAATGAAACCGAACTGAATACTCACAGTTGTGAGGGGGTGT
  AGAAGTTAACTTTCTGAGTGCTGTGGGTTTTGGGATGACATGAACCCTCAGGTATCTTTA
  AGGACTGGGGGATTTCTCTGTTAAGAGAAGGGCCTGATAACAACTCGAAAAGCAAGACTG
  CTTTGAAACTATACCCTGGGGATCTAAATGTTTCCATAAATGAAATCCGCTCCCTTCTCT
  AAAGAGCACANATCCTTCTTTGAGCGGATTGGNGGAAGGTATAAAAGGCCAATTATATCT
  TCATTTAACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032981
• Insert Size: 2340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032981.2, NP_116763.1
• RefSeq Size: 2181 bp
• RefSeq ORF: 579 bp
• Locus ID: 1837
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (8), also known as zeta transcript, lacks multiple exons from the 5' and 3' ends as well as few internal exons, and contains alternate 5' and 3' exons, compared to transcript variant 1. This results in a shorter isoform (8) with distinct N- and C-termini and lacking two internal segments, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.