EEF1D (NM_001960) Human Untagged Clone

CAT#: SC109188

EEF1D (untagged)-Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF1D
• Synonyms: EF-1D; EF1D; FP1047
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109188 sequence for NM_001960 edited (data generated by NextGen Sequencing)
  ATGGCTACAAACTTCCTAGCACATGAGAAGATCTGGTTCGACAAGTTCAAATATGACGAC
  GCAGAAAGGAGATTCTACGAGCAGATGAACGGGCCTGTGGCAGGKGCCTCCCGCCAGGAG
  AACGGCGCCAGCGTGATCCTCCGTGACATTGCGAGAGCCAGAGAGAACATCCAGAAATCC
  CTGGCTGGAAGCTCAGGCCCCGGGGCCTCCAGCGGCACCAGCGGAGACCACGGTGAGCTC
  GTCGTCCGGATTGCCAGTCTGGAAGTGGAGAACCAGAGTCTGCGTGGCGTGGTACAGGAG
  CTGCAGCAGGCCATCTCCAAGCTGGAGGCCCGGCTGAACGTGCTGGAGAAGAGCTCGCCT
  GGCCACCGGGCCACGGCCCCACAGACCCAGCACGTATCTCCCATGCGCCAAGTGGAGCCC
  CCAGCCAAGAAGCCAGCCACACCAGCAGAGGATGACGAGGATGATGACATTGACCTGTTT
  GGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGCGGGAGGAGCGGCTACGG
  CAGTACGCGGAGAAGAAGGCCAAGAAGCCTGCACTGGTGGCCAAGTCCTCCATCCTGCTG
  GATGTCAAGCCTTGGGATGATGAGACGGACATGGCCCAGCTGGAGGCCTGTGTGCGCTCT
  ATCCAGCTGGACGGGCTGGTCTGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATC
  CGGAAGCTACAGATTCAGTGTGTGGTGGAGGACGACAAGGTGGGGACAGACTTGCTGGAG
  GAGGAGATCACCAAGTTTGAGGAGCACGTGCAGAGTGTCGATATCGCAGCTTTCAACAAG
  ATCTGA
  
  Clone variation with respect to NM_001960.4
  105 t=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001960 unedited
  NGGGCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCCGAGGG
  TGCCCGGCGGGGCCGCAGAGACCGGCGGGGCCGCAACATCTTAGGGAACAAGCGGGCCGG
  GCTGCGACGGGCCGATGGGGAGGCCCCCTCTGCCTTGCCCTACTGTTACTTCCTGCAGAA
  GGATGCAGAGGCCCCCTGGCTCAGCAAGCCTGCCTACGACAGCGCCGAGTGCCGCCACCA
  CGCTGCCGAGGCCCTGCGTGTGGCCTGGTGCCTCGAAGCTGCCTCCCTGTCTCACCGACC
  CGGTCCTCGGTCTGGCCTGTCCGTGTCCAGCCTGAGACCCAACAGAAAAATGGCTACAAA
  CTTCCTAGCACATGAGAAGATCTGGTTCGACAAGTTCAAATATGACGACGCAGAAAGGAG
  ATTCTACGAGCAGATGAACGGGCCTGTGGCAGGTGCCTCCCGCCAGGAGAACGGCGCCAG
  CGTGATCCTCCGTGACATTGCGAGAGCCAGAGAGAACATCCAGAAATCCCTGGCTGGAAG
  CTCAGGCCCCGGGGCCTCCAGCGGCACCAGCGGAGACCACGGTGAGCTCGTCGTCCGGAT
  TGCCAGTCTGGAAGTGGAGAACCAGAGTCTGCGTGGCGTGGTACAGGAGCTGCAGCAGGC
  CATCTCCAAGCTGGAGGCCCCGCTGAACGTGCTGGAGAAGAGCTCGCCTGGCCACCGGGC
  CACGGCCCCACAGACCCCGCACGTATCTCCCATGCGCCCAGTGGAGCCCCCAGCCAAGAA
  GCCAGCCACACCANCANAGNATGACGAAGATGATGACCATTGACCTGTTTGCCAGTGACC
  ATGGAGGAGGAGGACCAGGAAGCGGCCCAGCTGCCGGAAGAAAGCGCTACCGCCAGTACC
  CGAAGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001960 unedited
  GACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTGCCGGGCTCAGTCTTTAATCGGGGGAGGGCCTTACGCACGCGCGCACGTACACACAC
  TTAGGCTTCAAATCTTGGTGAAAGCTGCGATATCGACACTTTGCACGTGCTCCTCAAACT
  TGGGGATCTCCTCCTCCAGCAAGTCTGTCCCCACCTTGGCGTCCTCCACCACACACTGAA
  TCTGTAGCTTCCGGATACCGGAACCCACGGGCACCAGCTTGGAAGCCCCCCAAACCAACC
  CGTCCAGCTGGATAAAGCGCACACAGGCCTCCAGCTGGGCCATGTCCGTCTCATCATCCC
  AAGGGTTGACATCCAGCAGGATGGAGGACTTGGCCACCAGTGCAGGCTTTTTGGCCTTTT
  TCTCCGCGTACTGGCGTAACCGCTCCTCCCGCAGCTGGGCCGCCTCCTTGGCCTCCTCCT
  CATTGGCACTGGCAAACAGGGCAATGGCATCATCCTCGGCATCCTCTGGTGGGGGGGCTG
  GCTTCTTGGCTGGGGGCTCCACTTGGCGCATGGGAGATACCTGCTGGGTCTGTGGGGCCG
  TGGCCCGGTGGCCAAGCGAGCTCTTCTCCAGCACGTTCAGCCGGGCCTTCAGCTTGGAGA
  TGGGCTGCTGCAACTTCTGTACCACGCCACGCAGACTCTGGTTCTTCACTTCCAGACTGG
  CAATCCCGACGACGAGCTCCCCGGGTCTCCCCTGGTGCCGCTGGAGGGCCCGGGGCCTCG
  AGCCTCCAACCAAGGATTTCTGGGAAGGTCTCTTCTGCCTCTCGCCATGGTCCCGGAGGA
  TCAAGCTTGGCGCCCGTCTCCTGGCGGGAAGCCACCTGGCCAAGCCCGGTTATTTGGTTC
  GGAAAACTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001960
• Insert Size: 1360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001960.2, NP_001951.2
• RefSeq Size: 994 bp
• RefSeq ORF: 846 bp
• Locus ID: 1936
• Cytogenetics: 8q24.3
• Domains: EF1BD
• Gene Summary: 'This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5, 6, and 9 encode the same isoform (2).