EIF4G1 (NM_004953) Human Untagged Clone

CAT#: SC109192

EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5


  "NM_004953" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EIF4G1
Synonyms EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109192 sequence for NM_004953 edited (data generated by NextGen Sequencing)
ATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAG
CCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCA
CAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAA
TCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCT
GAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCT
GTAGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAA
CCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCA
GAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTG
GAAATTCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCA
AGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCA
ACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGC
CCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAGTGGCGCCCCCCACC
ATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAA
ATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGT
GAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCT
CAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGG
AAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAG
GCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCA
GAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCA
AAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATAT
AAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTC
CTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATC
AGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGA
CTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACA
ACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCN
NNGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATC
ATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAA
CCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGC
AAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATG
TTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAA
GGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTAT
GCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTG
ACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAA
GATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAG
GAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCT
TTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATA
ATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTT
TGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATG
GATCAGTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATC
CGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGG
GATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGA
GAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCT
CCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTT
CCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGCCT
GGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAG
GGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCA
GCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACA
GATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGA
GACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTGAT
CGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGA
GAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGAC
CGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCG
GCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCAT
CTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTC
TTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAG
CATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTAC
TACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCCCAC
GTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATG
GGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTG
TTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTG
TGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCA
TTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAG
AGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGC
AGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAGATAGTATCC
AACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACT
CCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTG
TGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTA
GAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTG
AAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAG
GGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAG
TCTGACCACAACTGA

Clone variation with respect to NM_004953.4
706 a=>g;1500 g=>n;1501 c=>n;1502 a=>n
>OriGene 5' read for NM_004953 unedited
CCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
GGCACGAGGCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGG
GCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCT
AATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCA
ATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCT
TCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAAT
CTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAA
TCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCT
CTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAG
TTNTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCAT
GAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCCAGAGTGGAGTCAAGCCCAGAG
CTTGCTCCTCCCCAGCTTGCCCCTNCGATCCCCTGTGCCCATTGCTCCACTGCCCACCTG
NAGACTGCTCACGGAGCCCNCTCGCACCAGCTGNGGACTTAAGCCAGTCAGTGAGCCAGA
GGAGCAGCCAGGNNAGTGACAGCATCAGTGGNGCCCCCACCATCCCTNTGNTACTCAGTA
CGGNNTCTTAGCTACTTCCACTCAGAGGGAGGATGGGAAACAAANAAANAGGANAAGGNN
ACCNGGAAANCNNGAACCTGAT
>OriGene 3' read for NM_004953 unedited
TANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGAA
TTCATGTTTAATAATTACAGGCACCGTGCCCCCCCTTCCCCCTGCCCAGGCAACAACAGG
GGTGGTACAGGGGCTGGGGCATATGCCCCCAGCAGCGAGGACGGGAGTCCCGAGAGTGAT
TTTCAAAAAATATAATAAAGGACCCCAGGGGCAGGCGTTGGGGCCCCTCCCCACCCCAAG
ACACACCAAATTTCAAGACTTTATATATAATATATCTCTGTGCCCCAGGGGGAGGAAAGG
GACACCTGGCGGCATCCTGGAGGGGGGCCCAAGGAGCCCCAAGCCATCCTGCCTCTTCAA
CCACTTTATTAGTTTAAACACATTAACACTAAAGGGACCCACTGGCACCGCCGCTGGTGG
CGCCCCCCTGGAGTCCAAGGGGGTATCCGGGCCATCTGTGTGTCCCTGGGGCTTCCAGTT
CCCGGGCCCCCCAAACCTTCATTGGGGGCAAACCCCTTCTCTTGCTTCCGGAACCCCCTG
AAAAAGCTGTGACAATTTAAAGGCACACCCTTTGCCTGGTTGTTAAGGGGGGTCTTGCTT
CTCTCCCCACCTGTGAAAGGGATTCTCCTTTAACCAATTCTCTTTTTAAAAGGGGTCAAA
AAAAATCCCCCCAAAGGGGGGGGGGGTGTGCCTAAGGGTAACTAAAAGGGCCTCGAGGGC
GTAAAAAGCCCCTTGTTCCCTTTTTGTTCGGACACAAGGGTTTTTTTGGGAACCTTTCCT
TGTAGCTTTATTACCTCGGAGCCCCCCCGCCGGGGGGGATTTACCAAAAATATTCGAAAA
AATAAAACGCCCCCTACGGGGGTGAGTACCAC
Restriction Sites NotI-NotI     
ACCN NM_004953
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004953.2, NP_004944.2
RefSeq Size 5045 bp
RefSeq ORF 4212 bp
Locus ID 1981
Cytogenetics 3q27.1
Domains eIF5C, MIF4G, MA3
Protein Pathways Viral myocarditis
Gene Summary 'The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]'
Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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