EIF4G1 (NM_004953) Human Untagged Clone

CAT#: SC109192

EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4G1
• Synonyms: EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109192 sequence for NM_004953 edited (data generated by NextGen Sequencing)
  ATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAG
  CCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCA
  CAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAA
  TCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCT
  GAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCT
  GTAGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAA
  CCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCA
  GAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTG
  GAAATTCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCA
  AGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCA
  ACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGC
  CCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAGTGGCGCCCCCCACC
  ATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAA
  ATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGT
  GAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCT
  CAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGG
  AAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAG
  GCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCA
  GAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCA
  AAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATAT
  AAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTC
  CTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATC
  AGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGA
  CTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACA
  ACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCN
  NNGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATC
  ATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAA
  CCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGC
  AAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATG
  TTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAA
  GGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTAT
  GCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTG
  ACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAA
  GATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAG
  GAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCT
  TTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATA
  ATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTT
  TGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATG
  GATCAGTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATC
  CGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGG
  GATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGA
  GAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCT
  CCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTT
  CCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGCCT
  GGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAG
  GGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCA
  GCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACA
  GATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGA
  GACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTGAT
  CGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGA
  GAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGAC
  CGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCG
  GCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCAT
  CTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTC
  TTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAG
  CATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTAC
  TACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCCCAC
  GTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATG
  GGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTG
  TTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTG
  TGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCA
  TTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAG
  AGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGC
  AGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAGATAGTATCC
  AACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACT
  CCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTG
  TGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTA
  GAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTG
  AAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAG
  GGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAG
  TCTGACCACAACTGA
  
  Clone variation with respect to NM_004953.4
  706 a=>g;1500 g=>n;1501 c=>n;1502 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004953 unedited
  CCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
  TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
  GGCACGAGGCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGG
  GCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCT
  AATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCA
  ATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCT
  TCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAAT
  CTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAA
  TCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCT
  CTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAG
  TTNTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCAT
  GAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCCAGAGTGGAGTCAAGCCCAGAG
  CTTGCTCCTCCCCAGCTTGCCCCTNCGATCCCCTGTGCCCATTGCTCCACTGCCCACCTG
  NAGACTGCTCACGGAGCCCNCTCGCACCAGCTGNGGACTTAAGCCAGTCAGTGAGCCAGA
  GGAGCAGCCAGGNNAGTGACAGCATCAGTGGNGCCCCCACCATCCCTNTGNTACTCAGTA
  CGGNNTCTTAGCTACTTCCACTCAGAGGGAGGATGGGAAACAAANAAANAGGANAAGGNN
  ACCNGGAAANCNNGAACCTGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004953 unedited
  TANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGAA
  TTCATGTTTAATAATTACAGGCACCGTGCCCCCCCTTCCCCCTGCCCAGGCAACAACAGG
  GGTGGTACAGGGGCTGGGGCATATGCCCCCAGCAGCGAGGACGGGAGTCCCGAGAGTGAT
  TTTCAAAAAATATAATAAAGGACCCCAGGGGCAGGCGTTGGGGCCCCTCCCCACCCCAAG
  ACACACCAAATTTCAAGACTTTATATATAATATATCTCTGTGCCCCAGGGGGAGGAAAGG
  GACACCTGGCGGCATCCTGGAGGGGGGCCCAAGGAGCCCCAAGCCATCCTGCCTCTTCAA
  CCACTTTATTAGTTTAAACACATTAACACTAAAGGGACCCACTGGCACCGCCGCTGGTGG
  CGCCCCCCTGGAGTCCAAGGGGGTATCCGGGCCATCTGTGTGTCCCTGGGGCTTCCAGTT
  CCCGGGCCCCCCAAACCTTCATTGGGGGCAAACCCCTTCTCTTGCTTCCGGAACCCCCTG
  AAAAAGCTGTGACAATTTAAAGGCACACCCTTTGCCTGGTTGTTAAGGGGGGTCTTGCTT
  CTCTCCCCACCTGTGAAAGGGATTCTCCTTTAACCAATTCTCTTTTTAAAAGGGGTCAAA
  AAAAATCCCCCCAAAGGGGGGGGGGGTGTGCCTAAGGGTAACTAAAAGGGCCTCGAGGGC
  GTAAAAAGCCCCTTGTTCCCTTTTTGTTCGGACACAAGGGTTTTTTTGGGAACCTTTCCT
  TGTAGCTTTATTACCTCGGAGCCCCCCCGCCGGGGGGGATTTACCAAAAATATTCGAAAA
  AATAAAACGCCCCCTACGGGGGTGAGTACCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004953
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004953.2, NP_004944.2
• RefSeq Size: 5045 bp
• RefSeq ORF: 4212 bp
• Locus ID: 1981
• Cytogenetics: 3q27.1
• Domains: eIF5C, MIF4G, MA3
• Protein Pathways: Viral myocarditis
• Gene Summary: 'The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.