PYK2 (PTK2B) (NM_173176) Human Untagged Clone

CAT#: SC109211

PTK2B (untagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTK2B
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109211 sequence for NM_173176 edited (data generated by NextGen Sequencing)
  ATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACRTTACGCCGGCCTGAA
  GGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATC
  CTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAA
  TGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATC
  GGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGAT
  GAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTGT
  CTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTC
  ATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAAC
  GACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGC
  CTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAAC
  TTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAG
  AACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCG
  CTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATC
  GACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC
  ATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCC
  GAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTT
  CAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAG
  GCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGC
  TCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCC
  ATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGTCAGAC
  ATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATT
  GCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTAT
  GAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAG
  AAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAAC
  CTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCTGGATC
  ATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCC
  CTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTACCTG
  GAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCT
  GAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTAC
  AAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGA
  CGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGC
  TTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAA
  GGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGC
  TGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGT
  GACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGA
  ACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCT
  AAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCT
  GAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTT
  AACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGG
  GAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAA
  AAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAG
  TGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAMGTCCCCA
  TTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCG
  AGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTG
  TACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAG
  CTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTC
  ATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAG
  GGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCA
  CAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCA
  CACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTG
  GCCAATCTGGCCCACCCACCTGCAGAGTGA
  
  Clone variation with respect to NM_173176.2
  45 g=>r;2513 a=>m
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173176 unedited
  TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCGGC
  ACGAGGCTTTTACTCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGAC
  TTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAG
  TCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGG
  GGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGC
  AGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGT
  CTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGT
  CCAGACGGAGAATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCNGGATCGGGCCC
  AACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGATGAGATC
  CACTGGCTGCACCCACAGATGACGGTGGGTGGAGGGTGCNAGGACAAGTATGAGTGTCTG
  CACGTGGAAGCCGAGTGGGAAGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTCAT
  GGAGAGCCTGAANGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACG
  ACTACATGCAGCGCTACGCCAGCAAGGTCANCGAGGGCATGGCCCTGCAGCTGGGGCTGC
  CTGGACTTAAAGCCGGNTCTTCAAAGAATATGCCCCCACATGCACTTGAACAGAAGTTCA
  ACTTCGAGCTCTAGAAAGGGGAGAGGGGGGCTTGGCATCTGGGTTTTTCAAAAGCAGATG
  CAGGGAGACCTTAAGCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_173176 unedited
  AGAATCGAGAAAAAAAAAAAAAAAAACGCAAACGGGAAATAACCTACCTCAACCACATGC
  CCTACTAGATTGGATCCATTAGACGCGTCTTTGCCAAACACACGATAAAATGAAAGGGTG
  CAAACAGATAAACTGGGTCCTGGGGAATATATTAAATGAGGAGTTAAAATATGAGGGAAA
  AGCAAGGGAAAGTAAAGGAAATAGAGAAAGAGGGGCAGGAAGAGAGCGGATTTGGCCCAA
  GGTCCTATCTTGGCCGCATCTCTCTGCTTCTTCCCCCTGATGCTTTGGTTTGTTGACAAC
  ACAGCATCCTGTGCCTGGACTCCCAATTTAGCTTGTTCCTGGACTGTGCCCCAGGTCCTC
  CCTCAAGAGGGCACATGCTGTCAGTCCAGACCAAACTCACATTAAATAAATTTCAATATA
  CACTGTACAAGAATGCCAGGCCCATCCCTCATCTCACTGGCTGCCTGACCCCCAAAACAA
  AGCTCCTCCCCAGCTTCTCTGTGCATCAAGGGACATCTGAGGGCCGAAGAGGAAGGGAAA
  GGAATAGCTTGGTTCCCAAATCGGCCTGCCATGTCCATATATAAAGCTAGGGGCACCATG
  TGACACCTCCCCACCCCCAGGAGCCCCCTGCATGGACCAGGCTGGCTTAAGGAGCAGTGG
  CCAGGCAGCAGCAGTCCCCCTCTGAGCCATCTTTGTCACCGTCACCCTGTGTCCATAGCC
  CAGAATCCCCTCTGTCCAGCTGCAGCCTGAGCAGT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_173176
• ORF Size: 3030 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_173176.1, NP_775268.1
• RefSeq Size: 4089
• RefSeq ORF: 3030
• Locus ID: 2185
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.