FKBP12 (FKBP1A) (NM_000801) Human Untagged Clone

CAT#: SC109241

FKBP1A (untagged)-Human FK506 binding protein 1A, 12kDa (FKBP1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FKBP1A
• Synonyms: FKBP-1A; FKBP-12; FKBP1; FKBP12; PKC12; PKCI2; PPIASE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109241 sequence for NM_000801 edited (data generated by NextGen Sequencing)
  ATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGC
  CAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCC
  CGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGG
  GAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGAT
  TATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTC
  GATGTGGAGCTTCTAAAACTGGAATGA
  
  Clone variation with respect to NM_000801.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000801 unedited
  NGGTGCAGATTTGTATACGACTCACTATAGGCGGCNCGCGATTCGGCACGAGGTGCGCGT
  GCGCAGGCGACGCGCCNGAGTACTAGGCAGAGCCGTGGAACCGCCGCCAGGTCGCTGTTG
  GTCCACGCCGCCCGTCGCGCCGCCCGCCCGCTCAGCGTCCGCCGCCGCCATGGGAGTGCA
  GGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGT
  GGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAA
  CAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGT
  TGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGG
  TGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCT
  TCTAAAACTGGAATGACAGGAATGGCCTCCTCCCTTAGCTCCCTGTTCTTGGATCTGCCA
  TGGAGGGATCTGGTGCCTCCAGACATGTGCACATGAGTCCATATGGAGCTTTTCCTGATG
  TTCCACTCCACTTTGTATAGACATCTGCCCTGACTGAATGTGTTCTGTCACTCAGCTTTG
  CTTCCGACACCTCTGTTTCCTCTTTCCCTTTCTCCTCGTATGTGTGTTTACCTAAACTAT
  ATGCCATAAACCTCAAGTTACTCATTTTATTTTGTTTTCATTNTGGGGGTGAAGATTCGT
  TTTAGTCTTTTTGATTAGGTTTCCCATTAAGTACATGGTCAAGTATTACCAGCACAAGTG
  TTAGGCTAACACTACAATAGCATTGGTGTGGGGGGGGGGGGGTTCCCAAAAACCTCTAAT
  GTAATATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000801 unedited
  TTTTTTTTTTTTTTTTTGAGAAAACCGGCATAAAGCACTTTTATTGCAATAATAAAACTT
  GAGACTCATAAATGGTGCTGGGGGAAGGGTGCAGCAACGATTTCTCACCAAATCACTACA
  CAGGACAGCAAAGGGGTGAGAAGGGGCTGAGGGAGGAAAAGCCAGGAAACTGAGATCAGC
  AGAGGGAGCCAAGCATCAAAAAACAGGAGATGCTGAAGCTGCGATGACCAGCATCATTTT
  CTTAAGAGAACATTCAAGGATTTGTCATGATGGCTGGGCTTTCACTGGGTGTTAAGTCTA
  CAAACAGCACCTTCAATTGAAACTGTCAATTAAAGTTCTTAAGATTTAGGAAGTGGTGGA
  GCTTGGAAAGTTATGAGATTACAAAATTCCTGAAAGTCCATTAGAAAAACCACAGGATGA
  AAAAAAAAAAAGGCCACAAAGAAGGCTTCAGAGGTCCCTGCTGGCCCAGGGACAGATACC
  TGTAGTGTCCAGCATTGCAGTGAACATGATCTACTTTCAAAGGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000801
• Insert Size: 1630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000801.2, NP_000792.1
• RefSeq Size: 1578 bp
• RefSeq ORF: 327 bp
• Locus ID: 2280
• Cytogenetics: 20p13
• Domains: FKBP
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (1, also known as 12B) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode the same isoform (a).