FUT8 (NM_004480) Human Untagged Clone

CAT#: SC109248

FUT8 (untagged)-Human fucosyltransferase 8 (alpha (1,6) fucosyltransferase) (FUT8), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUT8
• Synonyms: CDGF; CDGF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109248 sequence for NM_004480 edited (data generated by NextGen Sequencing)
  ATGACGGATCTATACTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAG
  GCCAAAGATCTGACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAATCCCAAGGAC
  TGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAG
  CTCCATCATGTGGTCTACTGCTTCATGATTGCATATGGCACCCAGCGAACACTCATCTTG
  GAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTTAGGCCTGTAAGT
  GAGACATGCACAGACAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAAGTGAAGGAC
  AAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGTCCTCCATAT
  TTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCT
  GCAGTGTGGTGGGTGTCTCAGTTTGTCAAATACTTGATCCGCCCACAGCCTTGGCTAGAA
  AAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCAT
  GTCAGACGCACAGACAAAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAAGAGTACATG
  GTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAATGCAAGTGGACAAAAAAAGA
  GTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAGTACCCCAAT
  TATGAATTTATTAGTGATAACTCTATTTCCTGGTCAGCTGGACTGCACAATCGATACACA
  GAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTTCCTA
  GTGTGTACTTTTTCATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACAT
  CCTGATGCCTCTGCAAACTTCCATTCTTTAGATGACATCTACTATTTTGGGGGCCAGAAT
  GCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAAATTCCCATG
  GAACCTGGAGATATCATTGGTGTGGCTGGAAATCATTGGGATGGCTATTCTAAAGGTGTC
  AACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAGAAGATAGAA
  ACGGTCAAGTACCCCACATATCCTGAGGCTGAGAAATAA
  
  Clone variation with respect to NM_004480.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004480 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCACTGCTCAGGGTCGC
  GCGCCCTGGACCCAGCTCGCTCTCGGTCTCGCGCTGTCAGCGACTGCCCGGCTCGCGCCG
  CCTCGCGCTCTGCCTCAGTCAGTGGCGCCGAAGGCTCCGTTAAGCGGCGGCGGCGGTTCC
  TGTTTCCGTTTCTTCCTCTCCGTTCGGTCGGGAGTAGCATCCTCCACTCAGCCACCCTTC
  CCACTCCCCCATCGTGGGGCAGCTGCGGCTGAGGGCTGTGGCTTTGGCAGCTGCGACGGG
  GAGCGGCGGAGACCGCCTCTGCTCCCGCCTGGGGTTGCTGCTTTTGCTCAGAGGACATCC
  ATGACCCTAATGGTCTTTTTGTTCAAGATAAAGTGATTTTTTGCCTTTGTTGATTAACTG
  GACAAATTCAGCATGTAGAGCGCATGAAGTACAGGACAATAAAGCTTCCTACACATATCA
  CCAGGAGGATCTCTTTGAAAGATTCACTGCAGGACTACCAGAGAGAATAATTTGTCTGAA
  GCATCATGTGTTGAAACAACAGAAGTCTATTCACCTGTGCACTAACTAGAAACAGAGTTA
  CAATGTTTTCAATTCTTTGAGCTCCAGGACTCCAGGGAAGTGAGTTGAAAATCTGAAAAT
  GCGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTNTGCCTGNGGGACCCT
  TGCTGTTTTATATANGGTGGTCACTTTGGTACGAGATAATGACCATNCTGATCACTCTAG
  CCGAGAACTGTNNCAAGATCTGGNCAAGCTNGAACGCTTNANACAGCAGAATGAAGACTT
  TGAGCGAATGGCCCGAATCTCTNCCGATACCANAAGGCCCTATTGATCAGGNGCCAGCTA
  TAAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004480 unedited
  GCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTAAAA
  ATTAATTTTTTGTTATCAAAAAAAATGTCCAAAAACATTGAGTACTAAAAAATTTGTTCA
  TGGCATTGTTTTACAAAATTTTCTAATGGAAAGGGAATATTTTATTTTCTGATGTATTCT
  TCACTTATTAATGATGAGGTCAATGAACTGATGCAATTATTTAAAAAAAAAAAAACAGAC
  ACCCTTTTAAGGGAAAAACCAAAACCAAAACCAAAAAAAACCCAACCGTCCATTCTCATT
  CTGAATCTGTTGACTGGAGTGAATTCAGGGGTCACAATACCCCCATCAAATAAAAATCAA
  CTTCCTCAAAGTAAGGAGCTGTTCCGATCTTCTCAATAGTTCACACAGGTCTCTCAAATA
  TAGGCACCATGTTTTAAGTCTCACACAAAATGTCTGTGTCACTTAAATTACACAATATGG
  CAGTGTTTGTCTTATGGGGACATACTGAAAGGGGCAAAGTAAAAAACAACCTGTTTGCAT
  GTTATATGTGAGTACATTATTGAACTGTGCATGGGTATGAGGGCATTGCACCCCTTGTTA
  AAGAGGAATTCCACCAATTGAAGCCTATGTCAGTTCCCAGCTGCTCTTGGTGCTGAGAGT
  ATCTACTCATATAACCTTATTTTGTTAGATCAAAACCCTCTTCATCTACAGTTTGGCTGA
  AATGGTTTGAACTGAGTTTGGCAAACTGAGTTTGCCCGTTATCTCTTCCTCTGAGCTTAT
  TCTCACCCTCAGATTGGGGGGACTGACCGTTTTATCTTTTTAAACTTGAAGAGGATATAG
  CCCGTCTTCCAATTCTTGTGCCCCTTAAAAACCTCCCATGTTTCCACCCCCAAGATTCCC
  GGTCCTGAATTTTTTCCTTCGGGTGGGGCCATAAGGCATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004480
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004480.3, NP_004471.3
• RefSeq Size: 2963 bp
• RefSeq ORF: 1341 bp
• Locus ID: 2530
• Cytogenetics: 14q23.3
• Domains: SH3
• Protein Families: Transmembrane
• Protein Pathways: Keratan sulfate biosynthesis, Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (4) lacks a portion of the 5' UTR and 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript and protein data.