TFII I (GTF2I) (NM_033000) Human Untagged Clone

CAT#: SC109286

GTF2I (untagged)-Human general transcription factor IIi (GTF2I), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033000, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA
  CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCAT
  TGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAG
  GATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGA
  AATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGA
  GGACTATTTCTGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG
  ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAACACCCCGAGA
  ACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATTTCATTCATCATTAAGAGACC
  TTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATGGTAACAGATGCTGACAGGTCAATACTATCT
  CCAGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAA
  TGGCAGCTGTGACAGTAAAGGAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGGAAGCCA
  CCATTCTTCAGAGGGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGATTCTACTCAACATGTCCCT
  TCAGAAACAAGTGAGGACCCTGAAGTTGAGGTGACTATTGAAGATGATGATTATTCTCCACCGTCTAAGA
  GACCAAAGGCCAATGAGCTACCGCAGCCACCAGTCCCGGAACCCGCCAATGCTGGGAAGCGGAAAGTGAG
  GGAGTTCAACTTCGAGAAATGGAATGCTCGCATCACTGATCTACGTAAACAAGTTGAAGAATTGTTTGAA
  AGGAAATATGCTCAAGCCATAAAAGCCAAAGGTCCGGTGACGATCCCGTACCCTCTTTTCCAGTCTCATG
  TTGAAGATCTTTATGTAGAAGGACTTCCTGAAGGAATTCCTTTTAGAAGGCCATCTACTTACGGAATTCC
  TCGCCTGGAGAGGATATTACTTGCAAAGGAAAGGATTCGTTTTGTGATTAAGAAACATGAGCTTCTGAAT
  TCAACACGTGAAGATTTACAGCTTGATAAGCCAGCTTCAGGAGTAAAGGAAGAATGGTATGCCAGAATCA
  CTAAATTAAGAAAGATGGTGGATCAGCTTTTCTGCAAAAAATTTGCGGAAGCCTTGGGGAGCACTGAAGC
  CAAGGCTGTACCGTACCAAAAATTTGAGGCACACCCGAATGATCTGTACGTGGAAGGACTGCCAGAAAAC
  ATTCCTTTCCGAAGTCCCTCATGGTATGGAATCCCAAGGCTGGAAAAAATCATTCAAGTGGGCAATCGAA
  TTAAATTTGTTATTAAAAGACCAGAACTTCTGACTCACAGTACCACTGAAGTTACTCAGCCAAGAACGAA
  TACACCAGTCAAAGAAGATTGGAATGTCAGAATTACCAAGCTACGGAAGCAAGTGGAAGAGATTTTTAAT
  TTGAAATTTGCTCAAGCTCTTGGACTCACCGAGGCAGTAAAAGTACCATATCCTGTGTTTGAATCAAACC
  CGGAGTTCTTGTATGTGGAAGGCTTGCCAGAGGGGATTCCCTTCCGAAGCCCTACCTGGTTTGGAATTCC
  ACGACTTGAAAGGATCGTCCGCGGGAGTAATAAAATCAAGTTCGTTGTTAAAAAACCTGAACTAGTTATT
  TCCTACTTGCCTCCTGGGATGGCTAGTAAAATAAACACTAAAGCTTTGCAGTCCCCCAAAAGACCACGAA
  GTCCTGGGAGTAATTCAAAGGTTCCTGAAATTGAGGTCACCGTGGAAGGCCCTAATAACAACAATCCTCA
  AACCTCAGCTGTTCGAACCCCGACCCAGACTAACGGTTCTAACGTTCCCTTCAAGCCACGAGGGAGAGAG
  TTTTCCTTTGAGGCCTGGAATGCCAAAATCACGGACCTAAAACAGAAAGTTGAAAATCTCTTCAATGAGA
  AATGTGGGGAAGCTCTTGGCCTTAAACAAGCTGTGAAGGTGCCGTTCGCGTTATTTGAGTCTTTCCCGGA
  AGACTTTTATGTGGAAGGCTTACCTGAGGGTGTGCCATTCCGAAGACCATCGACTTTTGGCATTCCGAGG
  CTGGAGAAGATACTCAGAAACAAAGCCAAAATTAAGTTCATCATTAAAAAGCCCGAAATGTTTGAGACGG
  CGATTAAGGAGAGCACCTCCTCTAAGAGCCCTCCCAGAAAAATAAATTCATCACCCAATGTTAATACTAC
  TGCATCAGGTGTTGAAGACCTTAACATCATTCAGGTGACAATTCCAGATGATGATAATGAAAGACTCTCG
  AAAGTTGAAAAAGCTAGACAGCTAAGAGAACAAGTGAATGACCTCTTTAGTCGGAAATTTGGTGAAGCTA
  TTGGTATGGGTTTTCCTGTGAAAGTTCCCTACAGGAAAATCACAATTAACCCTGGCTGTGTGGTAGTTGA
  TGGCATGCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTACCTGGAAATCAGCTCCATGAGAAGGATCTTA
  GACTCTGCCGAGTTTATCAAATTCACGGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGG
  TTGATCAGAGTGAGTCAGAAGGCCCCGTGATACAAGAATCAGCTGAACCAAGCCAGTTGGAAGTTCCAGC
  CACAGAAGAAATAAAAGAGACTGATGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033000 unedited
  NGGGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACCANANAGAAAA
  GGGCCACCGGTCGCCCCCCCGCTTCCCCGCACGCGCTCTCCAGCCGCGGCCGCCCGCCTG
  CCGCGGTCACCCCGGCCTCTGCCTCTGTCCCCCAGTGATCGGATCAAGGCGCTGAGCGAG
  GCCCTGCCTGCGGGGCGGCCATGCGGCGGTGACAGGAGCGCGACCGACACGCACGGGCCC
  CTCGCCCCCTCTCGCCTCCCGTCCGCTCGCCAGCTCCCCTCAGCCGAGGCTGCTCCGCGG
  CGGCCGCAGCCCGCGCGCGGCCCACACTCGCCTCCCCTCGGCACCCCCGGCCCCGGAGCT
  GCCTGGAGGCGGCCGCACTCGGGGATCATGGCCCAAGTTGCAATGTCCACCCTCCCCGTT
  GAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGACATTCCTCATGTCAGCTCTCGAG
  TCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCATTGCAGTGTATGAA
  ACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAGGAT
  TTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCAT
  AAAATGAAATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACA
  CTCAGAANAACAGTTGAGGACTATTTCTGCTTTTGCTATGGGAAAGCTNTANGCAAATCC
  ACAGTGGTACCTGTACCATATGAGAAGATGCTGCGAGACCCAGTCGCTGTGGTAGTGCAG
  GNGCTTCCGGAAGGTGTTGCCTTTAAAACACCCGAAACTATGATCCTGCACCCTGAAATG
  GATTTGGAGAACAAGCAGGNATTCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033000 unedited
  GGGGTACCTGTGNACCGCGCGCCGCATTCTAGNATCGAGTAATTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTAGGGGAACCAAATGGTTTTGCTTTTAATTATGATAAAAA
  AACATAGGAACCATGAAAGATAGCTTAGGAAAAGGTTTAGTCAAATATACAGATAGGAAC
  TGTTCACTCAGTCATTAAGGAGAGCCGGGAACAAAGGATCATTTAAATGATTTTTTTTTT
  TCAAATAGAAAAACTAAAGAACTGAACTATCACAGGAATTTCTTTTCACTGCAAAACCTG
  AAATGTTCCAGTAGGAAAAGTAGGAAATATCTTCACTTGATTGGAAAATAAACACCGTAA
  CTCAAGGCTCTGTTGGGTACATAAAAAATCGGGGGAGATTCATTCCTGGCATTTCCCCTG
  TCATTCCCCCCCATTGGTGGAAAATCCTGATAGGCCCCTGCCCAGTCCCCCCGCATTCCA
  AACCCACCGCCACTCCGCTTTCTAATCGTAGAACTGCCTGTACAGCGCCTCCCGTCTTTC
  CACCACCACCACAGGCCCCTTCCCAAGTTCCTCGCTCCCCCCTCTTCCCACCCCCCCGGC
  CCTCCTTTGTTCTCCACCCGTATCCCGTCACCTTTCCCTACCTTCCTTGCGGCCCCTACC
  CCTTCCCCCCCCCTCCCTCCTCCCAGGTCTTTTCGCCCCCCTCCCCCCCTCTTCTTCCCC
  CCCCCGCTCTTTTCCTCTTCCGTTTTCTTCTCCTCCTTCCCTCACCCTCCCTTCCTTTCC
  TTCTCCCATCCCTCCTGTTCCCCTCTCCTACCCCTACCTCTTTCTTTCCCCTCCTCGCTT
  CCCCTTCTTTTTTCTCTTACCCCACCCCTCTCTCCCTTTCCTCACCCCCCCACACCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033000
• Insert Size: 3750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033000.1, NP_127493.1
• RefSeq Size: 4520 bp
• RefSeq ORF: 2937 bp
• Locus ID: 2969
• Cytogenetics: 7q11.23
• Domains: GTF2I
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2, also known as beta) is shorter than isoform 1.