HSD11B1 (NM_005525) Human Untagged Clone

CAT#: SC109325

HSD11B1 (untagged)-Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD11B1
• Synonyms: 11-beta-HSD1; 11-DH; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109325 sequence for NM_005525 edited (data generated by NextGen Sequencing)
  ATGGCTTTTATGAAAAAATATCTCCTCCCCATTCTGGGGCTCTTCATGGCCTACTACTAC
  TATTCTGCAAACGAGGAATTCAGACCAGAGATGCTCCAAGGAAAGAAAGTGATTGTCACA
  GGGGCCAGCAAAGGGATCGGAAGAGAGATGGCTTATCATCTGGCGAAGATGGGAGCCCAT
  GTGGTGGTGACAGCGAGGTCAAAAGAAACTCTACAGAAGGTGGTATCCCACTGCCTGGAG
  CTTGGAGCAGCCTCAGCACACTACATTGCTGGCACCATGGAAGACATGACCTTCGCAGAG
  CAATTTGTTGCCCAAGCAGGAAAGCTCATGGGAGGACTAGACATGCTCATTCTCAACCAC
  ATCACCAACACTTCTTTGAATCTTTTTCATGATGATATTCACCATGTGCGCAAAAGCATG
  GAAGTCAACTTCCTCAGTTACGTGGTCCTGACTGTAGCTGCCTTGCCCATGCTGAAGCAG
  AGCAATGGAAGCATTGTTGTCGTCTCCTCTCTGGCTGGGAAAGTGGCTTATCCAATGGTT
  GCTGCCTATTCTGCAAGCAAGTTTGCTTTGGATGGGTTCTTCTCCTCCATCAGAAAGGAA
  TATTCAGTGTCCAGGGTCAATGTATCAATCACTCTCTGTGTTCTTGGCCTCATAGACACA
  GAAACAGCCATGAAGGCAGTTTCTGGGATAGTCCATATGCAAGCAGCTCCAAAGGAGGAA
  TGTGCCCTGGAGATCATCAAAGGGGGAGCTCTGCGCCAAGAAGAAGTGTATTATGACAGC
  TCACTCTGGACCACTCTTCTGATCAGAAATCCATGCAGGAAGATCCTGGAATTTCTCTAC
  TCAACGAGCTATAATATGGACAGATTCATAAACAAGTAG
  
  Clone variation with respect to NM_005525.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005525 unedited
  ACGAGGAGNAAGTGTGATGAAACATAAGTAAAGCAAATGCCCGAGGGAGAGAGAGAGAAG
  AGAAGAAAAATATAAAAGAACATCAATAAAAAGAAGTCAGATTTGTTCGAAATCTTGAGG
  AGTCTTCAGGCCAGCTCCCTGTCGGATGGCTTTTATGAAAAAATTTTTTTCCCCATTCTG
  GGGCTCTTCATGGCCTACTACTACTATTCTGCAAACGAGGAATTCAGACCAGATATGCTC
  CAAGGAAATAAAGTGATTGTCACAGGGGCCAGCAAAGGGATCGGAAGATAGATGGCTTAT
  CATCTGGCGAATATGGGAGCCCATGTGGTGGTGACAGCGAGGTCAAAAGAAACTCTACAG
  AAGGTGGTATCCCACTGCCTGGAGCTTGGAGCAGCCTCAGCACACTACATTGCTGGCACC
  ATGGAAGACATGACCTTCGCAGAGCAATTTGTTGCCCAAGCAGGAAAGCTCATGGGAGGA
  CTAGACATGCTCATTCTCAACCACATCACCAACACTTCTTTGAATCTTTTTCATGATGAT
  ATTCACCATGTGCGCAAAAGCATGGAAGTCAACTTCCTCAGTTACGTGGTCCTGACTGTA
  GCTGCCTTGCCCATGCTGAAGCAGAGCAATGGAAGCATTGTTGTCGTCTCCTCTCTGGCT
  GGGAAAGTGGCTTATCCAATGGTTGCTGCCTATTCTGCAAGCAAGTTTGCTTTGGATGGG
  TTCTTCTCCTCCTCAGAAAGGATATTCAGTGTCCCAGGTCAAGGATCAATCACTCTCTGG
  GTTCTTGGCCTCATAGACACCAGAACAGCCCTGAAAGCAGTTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005525 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTGTACAGTTTATGGTTTT
  AAAGAAACCATCCTGAATAAGCTCAAGTTATAGCTACCAGTTATGAATTTATAAAGTTTA
  TGTGACCTTTATTATAATTAATATTGTATTAATCATCACATATTATTATATATAAGATAT
  AAATTTAATATAACTATCTGGTAATTATACCTATGTTTACTAACAATTTTACAACTGCTG
  GCTGCAGCGGTGCATGACATTCATTATTATTACATTTCCATTTTGCAAATGTTAAAGGAA
  CTCCTTCCATTTGTCAGGTGTGACCCATGACTTGCATGTCTCTGGGGACACTCTGGGAAG
  ATGTCTTCATAGATAAGAGCTCAGATAAACATGAGACAGAACAGTCCCAAAATCCCTCAG
  CATGCCCAGCCCTCAGGGAGTTCCTACTTGTTTATGAATCTGTCCATATTATAGCTCGTT
  GAGTAGAGAAATTCCAGGATCTTCCTGCATGGATTTCTGATCAGAAGAGTGGTCCAGAGT
  GAGCTGTCATAATACACTTCTTCTTGGCGCAGAGCTCCCCCTTTGATGATCTCCAGGGCA
  CATTCCTCCTTTGGAGCTGCTTGCATATGGACTATCCCAGAAACTGCCTTCATGGCTGTT
  TCTGTGTCTATGAGGCCAAGAACACAGAGAGTGATTGATACATTGACCCTGGACACTGAA
  TATTCCTTTCTGATGGAGGAGAAGAACCCATNCCAAGCAAACTTGCTTGCAGAATAGGCA
  GCAACCCATTGGATAAGCCACTTTCCCAGCCAGNAGANAGACGACAAACCATGCTTCATT
  GCTCTGCTTCAGCATGGGCAAGGCAGCTACAGTCAGGAACACNNGTACTGAGAAAGTGAC
  TTCCATGCTTTGCGCCATGGTGAATATCCTCTGAAAGATCCAAGAGTGTGTGATGGGTGA
  AAATGACTGCTATCCTCCATGACTTCTGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005525
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005525.2, NP_005516.1
• RefSeq Size: 1405 bp
• RefSeq ORF: 879 bp
• Locus ID: 3290
• Cytogenetics: 1q32.2
• Domains: adh_short
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2 and 3 encode the same protein.