KRAS (NM_004985) Human Untagged Clone

CAT#: SC109374

KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b


  "NM_004985" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KRAS
Synonyms C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_004985 edited
GAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGG
CGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCA
GGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGCC
TGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTG
CCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGG
ATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGACA
CAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCT
TTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAG
AACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAAT
GTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATG
GAATTCCTTTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATA
CATTAGTTCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGA
AAAAGAAGTCAAAGACAAAGTGTGTAATTATGTAAATACAATTTGTACTTTTTTCTTAAG
GCATACTAGTACAAGTGGTAATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAG
CATTACCTAATTTTTTTCCTGCTCCATGCAGACTGTTAGCTTTTACCTTAAATGCTTATT
TTAAAATGACAGTGGAAGTTTTTTTTTCCTCGAAGTGCCAGTATTCCCAGAGTTTTGGTT
TTTGAACTAGCAATGCCTGTGAAAAAGAAACTGAATACCTAAGATTTCTGTCTTGGGGTT
TTTGGTGCATGCAGTTGATTACTTCTTATTTTTCTTACCAATTGTGAATGTTGGTGTGAA
ACAAATTAATGAAGCTTTTGAATCATCCCTATTCTGTGTTTTATCTAGTCACATAAATGG
ATTAATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGAGGGAAC
ACAAATTTATGGGCTTCCTGATGATGATTCTTCTAGGCATCATGTCCTATAGTTTGTCAT
CCCTGACGAATGTAAAGXXXXXXXXXXXXXXXXXXXXXXXXAATGGTTTCTTGATAATTG
TGTAGTAATGTTTTTTAGAACCCAGCAGTTACCTTAAAGCTGAATTTATATTTAGTAACT
TCTGTGTTAATACTGGATAGCATGAATTCTGCATTGAGAACCTGAATAGCTGTCATAAAA
TGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAAGAATAGTCATAACTAG
ATTAAGATCTGTGTTTTAGTTTAATAGTTTGAAGTGCCTGTTTGGGATAATGATAGGTAA
TTTAGATGAATTTAGGGGAAAAAAGTTATCTGCAGAAATGTTGAGGGCCCATCTCTCCCC
CCACACCCCCACAGAGCTAACTGGGTTACAGTGTTTTATCCGAAAGTTTCCAATTCCACT
GTCTTGTGTTTTCATGTTGAAAATACTTTTGCATTTTTCCTTTGAGTGCCAATTTCTTAC
TAGTACTATTTCTTAATGTAACATGTTTACCTGGAATGTATTTTAACTATTTTTGTATAG
TGTAAACTGAAACATGCACATTTTGTACATTGTGCTTTCTTTTGTGGGACATATGCAGTG
TGATCCAGTTGTTTTCCATCATTTGGTTGCGCTGACCTAGGAATGTTGGTCATATCAAAC
ATTAAAAATGACCACTCTTTTAATTGAAATTAACTTTTAAATGTTTATAGGAGTATGTGC
TGTGAAGTGATCTAAAATTTGTAATATTTTTGTCATGAACTGTACTACTCCTAATTATTG
TAATGTAATAAAAATAGTTACAGTGACAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_004985 unedited
TATTACACCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
CGAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTG
GCGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGC
AGGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGC
CTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGT
GCCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAG
GATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGAC
ACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGC
TTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGA
GAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAA
TGTGATTTGCCTTCTAGAACAGTAGACACANAACAGGCTCAGGACTTAGCAAGAAGTTAT
GGAATTCCTTNTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTAT
ACATTAGNTCGAGAAATTNCGAAACATANAGAANAGATGAGCANAGATGGGTAAAAGAAG
AAAAAGAAGTCNAAGACAAGTGTGTAANTATGTAAATACCATTNGTACTTTTNTCTTAAG
CATACTAGTACAAGGGGTAATTTTTGTACATTACACTAAATT
>OriGene 3' read for NM_004985 unedited
NGGGAAATCTATGNACCGCGCCGCATTCTANGTCGAGTTTTTTTTTTTTTTTTTTGTCAC
TGTAACTATTTTTATTACATTACAATAATTAGGAGTAGTACAGTTCATGACAAAAATTTA
CAAATTTTAGATCACTTCACAGCACATACTCCTATAAACATTTAAAAGTTAATTTCAATT
AAAAGAGTGGTCATTTTTAATGTTTGATATGACCAACATTCCTAGGTCAGCGCAACCAAA
TGAATGAAAACAACTGGATCACACTGCATATGTCCCACAAAAGAAAGCACAATGTACAAA
ATGTGCATGTTTCAGTTTACACTATACAAAAATAGTTAAAATACATTCCAGGTAAACATG
TTACATTAAGAAATAGTACTAGTAAGAAATTGGCACTCAAAGGAAAAATGCAAAAGTATT
TTCAACATGAAAACACAAGACAGTGGAATTGAAAACTTTCGGATAAAACACTGTAACCCA
GTTAGCTCTGTGGGGGTGTGGGGGGAAGAGATGGGCCCTCAACATTTCTGCAGATAACTT
TTTTCCCCTAAATTCATCTAAATTACCTATCATTATCCCAAACAGGCACTTCAAACTATT
AAACTAAAACACAGATCTTAATCTAGTTATGACTATTCTTCAAGAACTCATGTGAGTATC
TTTCTTTAGAAAGAAAGTTTCATTTTATGACAGCTATTCACGTTCTCAATGCAGAATTCA
TGCTATCCAGTATTAACACAGAAAGTACTAAATATAAATTCAGCTTTAAGGTAACTGCTG
GGTNTCTAAAAACATTTCTACACAATTATCAAGAAACATTACTTTTTGACAATGGGAATC
TTAAATAGTTTTGCTGTCTAAAAAAAATCCCTAAAAAGTTATTACTGGTTGAAGAAAAAT
GTTAGA
Restriction Sites NotI-NotI     
ACCN NM_004985
Insert Size 2067 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004985.3, NP_004976.2
RefSeq Size 5312 bp
RefSeq ORF 567 bp
Locus ID 3845
Cytogenetics 12p12.1
Domains ras, RAS, RHO, RAB
Protein Families Druggable Genome
Protein Pathways Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
Gene Summary 'This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (b) is composed of five exons and lacks exon 4a which the longer transcript variant (a) includes. This predominant variant (b) has a cds that terminates in exon 4b and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.