DNA Ligase III (LIG3) (NM_013975) Human Untagged Clone

CAT#: SC109386

LIG3 (untagged)-Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha


  "NM_013975" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LIG3
Synonyms LIG2; LIG3alpha
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_013975 edited
ATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTT
CCGAGGCCTACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGA
TCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAAC
ATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGAC
ATCCCCTCTTCCTGAGAAGAAAGCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCAC
GTGCCACCTACCTTGTTTTCTTGCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTG
AGATGGCTGAGCAACGGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAA
AATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCT
TCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAAC
TAGAGCGGGCCCGGGCCACCACAAAAAAAATCGAGGACCTCACAGAGCTGGAAGGCTGGG
AAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTA
AGGCAGCAGGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCC
AGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATCCCCGGAAATTTT
CTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGA
GAAGTCTGTCTTCAAGCAAATGTGACCCCAGGCATAAGGACTGTCTGCTACGG:GAGTTT
CGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATC
CAGGACTTCCTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGTACCTAACA
GTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACGATAAGCAGATT
GTGAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAG
CAGGGTGACGTGTCAGAGACAATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCA
GCTGCCAAGAGCCTCCTTACCATCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAG
CTCACCAAGGAGGATGAGCAGCAACAGGCCCTACAGGACATTGCCTCCAGGTGTACAGCC
AATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTGAAGATGAACTCAGGTGCA
AAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCTCGCGCAAC
CTGCAGGATGTGGTGGAGCGGGTCCTTCACAACGCGCAGGAGGTGGAGAAGGAGCCGGGC
CAGAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCG
GAGGCCTGCAAGTCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAG
ATCAAGTACGATGGAGAGCGAGTCCAGGTGCATAAGAATGGAGACCACTTCAGCTACTTC
AGCCGCAGTCTCAAGCCCGTCCTTCCTCACAAGGTGGCCCACTTTAAGGACTACATTCCC
CAGGCTTTTCCTGGGGGCCACAGCATGATCTTGGATTCTGAAGTGCTTCTGATTGACAAC
AAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAGCCTTCCAG
GATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATG
GACAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTGAAATTCCAAAC
CGGATCATGTTCTCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATA
ACCCGGGTGATCCAGGAGGGATTGGAGGGGCTGGTGCTGAAGGATGTGAAGGGTACATAT
GAGCCTGGGAAGCGGCACTGGCTGAAAGTGAAGAAAGACTATTTGAACGAGGGGGCCATG
GCCGACACAGCTGACCTGGTGGTCCTTGGAGCCTTCTATGGGCAAGGGAGCAAAGGCGGC
ATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGTGGTGCACAGTC
ACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGAC
ATGGTGAAGATCAGCAAGGACCCCAGCAAAATACCCAGCTGGTTGAAGGTCAACAAGATC
TACTATCCTGACTTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGG
GCTGAATTCTCCAAATCGGAGGCTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGC
TGCACCCGAATCCGAGATGATAAGGACTGGAAATCTGCCACTAACCTTCCCCAACTCAAG
GAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTAGTGGCTGGAGATGAGGGG
AGCTCCACTACAGGGGGTAGCAGTGAAGAGAATAAGGGTCCCTCAGGGTCTGCTGTGTCC
CGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAAC
TCCAACAGCAAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAG
AAGCTGGCCACAAAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAGCTGCTGATGAG
ACGCTGTGCCAAACAAAGGTATTGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCA
CCCTCCACACCAGACTTCAGCCGTCTCAGACGCTACTTTGTGGCATTCGACGGGGACCTG
GTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGTAGCAGGGACAAGAACCCT
GCGGCCCAGCAGGTCTCCCCAGAGTGGATTTGGGCATGTATCCGGAAACGGAGACTGGTA
GCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCCTTTACCATACT
ACTGGACTGGACTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTC
TCCCAAACCCACCAGTTCTCCACTGTCTCTTCTGGACCAGGAATTAGTTGCTGTGGGTGC
CACAGCTGAAGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGG
TTTGCCATCTTTTTGTTTTCTTTGAAAAGCAGCTTAGTTACCCTTTTTATAAATAAAATA
TCTTGCAGTAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_013975 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTTAAAGAGACGGCGCTCCA
ACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCCTACGGTGAGCGCCG
GAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCG
TGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAACATCACTGGCGTGATGTAAGACA
ATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAA
GCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTT
GCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCTGAGCAACGGTTCTG
TGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAA
GGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCTTCTCAGAGTCTGGGGGTGATAT
GAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCAC
AAAAAATATCGAGGACCTCACAGAGCTGGAAGGCTNGGAAGAGCTGGAAAGATATGAGAA
GGAACAGANTACCCAGCACATTGCAGATCTGTCTTCTAGGCAGCAGGTACACCAAAGAAG
AAAGCTGGTGTCCAGNCTAAAAGTGACACCACTGGNCCAGTGACTTCTCCAGTGAAAGGC
GCCTCATTTGTCACCAGTACCAATCCCCGGATATTNTCTGGNCTTTTCAGCCCAGCCCCA
TCACTCTGGGGGAATGCCCNCTCGAGCCCACCCCTAAGAAGAAGTCTGTCTTCAGCAAAT
GTGACCCCAGCATAAGGACTGCTGCTCACGNAGTTTTCCAAAGTCTGCCCCATGGNGGNC
GATATCCTATCTCACACGAGAACCAGATCTCCAGACTTCTTCGAAGCTANAAGGG
>OriGene 3' read for NM_013975 unedited
GACCGCGGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTACTGCAAGATATTTT
ATTTATAAAAAGGGTAACTAAGCTGCTTTTCAAAGAAAACAAAAAGATGGCAAACCCAGC
ACCCAGCTCTGAAAGATCTATTTAAACCAGCAAGACAAACTGACTTCAGCTGTGGCACCC
ACAGCAACTAATTCCTGGTCCAGAAGAGACAGTGGAGAACTGGTGGGTTTGGGAGAAGCA
AGCCCATTCCCCCTATACTGTGTCTATCTGCCTCCAGCCTGAGTCCAGTCCAGTAGTATG
GTAAAGGAGAGTATGGCCTGAGGGAGAGGGAAGACAGCAAACCTAGCAGGGAGCTACCAG
TCTCCGTTTCCGGATACATGCCCAAATCCACTCTGGGGAGACCTGCTGGGCCGCAGGGCT
CTTGCCCCTGCTACCCAGCACGTGCGTGGCTGAAGTCATATCAAATCCCTGTACCACGCC
CCCGTCGAATGCCACAAAGTATCGCCCGACACGGCTGAAGTCTGGTGTGGAGGGTGGTAA
GTAAAGCCGCACCCCAGTGAAGATGTCCAGCAATACCTTTGTTTGGCACAGCGTCTCATC
ATCAGCTTCTCCGCTTCTCCCCTACTTTCACTGCAGAAGACTTTGTGGCCAGCTTTTCCC
CCACCTTCATAGCCGAAGGCTTTGCAGCCTGCATGCCGCCATCCCTCGCTGTTGGAGTTA
CCCAACTTCCCCTTTCCTTTCCTTGTCACTGGCTGCGGGCCTGCTTGGGGGCCCCGCCGG
ACACAGCACACCCCTGAGGACCCTNACTTTTCTCCACTGGCTACCCCCTGGAGCGCAGCT
CCCCTTAATCTCCGCCCCTAACGTGAAGTCCGCCTTCATCCTTGGACAACCGGACCACGC
TTCCTCGATCTCGGGCACAGTTACCGTTATACATTCCATCCTTCACATCTCGGAT
Restriction Sites NotI-NotI     
ACCN NM_013975
Insert Size 3510 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013975.2, NP_039269.2
RefSeq Size 3701 bp
RefSeq ORF 3030 bp
Locus ID 3980
Cytogenetics 17q12
Domains DNA_ligase, BRCT, zf-PARP
Protein Families Druggable Genome
Protein Pathways Base excision repair
Gene Summary 'This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha).

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