MAP4 (NM_002375) Human Untagged Clone

CAT#: SC109401

MAP4 (untagged)-Human microtubule-associated protein 4 (MAP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4
• Synonyms: DKFZp779A1753; MGC8617
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002375, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGACCTCAGTCTTGCAGATGCATTAACAGAACCATCTCCAGACATTGAGGGAGAGATAAAGCGGG
  ACTTCATTGCCACACTAGAGGCAGAGGCCTTTGATGATGTTGTGGGAGAAACTGTTGGAAAAACAGACTA
  TATTCCTCTCCTGGATGTTGATGAGAAAACCGGGAACTCAGAGTCAAAGAAGAAACCGTGCTCAGAAACT
  AGCCAGATTGAAGATACTCCATCTTCTAAACCAACACTCCTAGCCAATGGTGGTCATGGAGTAGAAGGGA
  GCGATACTACAGGGTCTCCAACTGAATTCCTTGAAGAGAAAATGGCCTACCAGGAATACCCAAATAGCCA
  GAACTGGCCAGAAGATACCAACTTTTGTTTCCAACCTGAGCAAGTGGTCGATCCTATCCAGACTGATCCC
  TTTAAGATGTACCATGATGATGACCTGGCAGATTTGGTCTTTCCCTCCAGTGCGACAGCTGATACTTCAA
  TATTTGCAGGACAAAATGATCCCTTGAAAGACAGTTACGGTATGTCTCCCTGCAACACAGCTGTTGTACC
  TCAGGGGTGGTCTGTGGAAGCCTTAAACTCTCCACACTCAGAGTCCTTTGTTTCCCCAGAGGCTGTTGCA
  GAACCTCCTCAGCCAACGGCAGTTCCCTTAGAGCTAGCCAAGGAGATAGAAATGGCATCAGAAGAGAGGC
  CACCAGCACAAGCATTGGAAATAATGATGGGACTGAAGACTACTGACATGGCACCATCTAAAGAAACAGA
  GATGGCCCTCGCCAAGGACATGGCACTAGCTACAAAAACCGAGGTGGCATTGGCTAAAGATATGGAATCA
  CCCACCAAATTAGATGTGACACTGGCCAAGGACATGCAGCCATCCATGGAATCAGATATGGCCCTAGTCA
  AGGACATGGAACTACCCACAGAAAAAGAAGTGGCCCTGGTTAAGGATGTCAGATGGCCCACAGAAACAGA
  TGTATCTTCAGCCAAGAATGTGGTACTGCCCACAGAAACAGAGGTAGCCCCAGCCAAGGATGTGACACTG
  TTGAAAGAAACAGAGAGGGCATCTCCTATAAAAATGGACTTAGCCCCTTCCAAGGACATGGGACCACCCA
  AAGAAAACAAGAAAGAAACAGAGAGGGCATCTCCTATAAAAATGGACTTGGCTCCTTCCAAGGACATGGG
  ACCACCCAAAGAAAACAAGATAGTCCCAGCCAAGGATTTGGTATTACTCTCAGAAATAGAGGTGGCACAG
  GCTAATGACATTATATCATCCACAGAAATATCCTCTGCTGAGAAGGTGGCTTTGTCCTCAGAAACAGAGG
  TAGCCCTGGCCAGGGACATGACACTGCCCCCGGAAACCAACGTGATCTTGACCAAGGATAAAGCACTACC
  TTTAGAAGCAGAGGTGGCCCCAGTCAAGGACATGGCTCAACTCCCAGAAACAGAAATAGCCCCGGCCAAG
  GATGTGGCTCCGTCCACAGTAAAAGAAGTGGGCTTGTTGAAGGACATGTCTCCACTATCAGAAACAGAAA
  TGGCTCTGGGCAAGGATGTGACTCCACCTCCAGAAACAGAAGTAGTTCTCATCAAGAACGTATGTCTGCC
  TCCAGAAATGGAGGTGGCCCTGACTGAGGATCAGGTCCCAGCCCTCAAAACAGAAGCACCCCTGGCTAAG
  GATGGGGTTCTGACCCTGGCCAACAATGTGACTCCAGCCAAAGATGTTCCACCACTCTCAGAAACAGAGG
  CAACACCAGTTCCAATTAAAGACATGGAAATTGCACAAACACAAAAAGGAATAAGTGAGGATTCCCATTT
  AGAATCTCTGCAGGATGTGGGGCAGTCAGCTGCACCTACTTTCATGATTTCACCAGAAACCGTCACAGGA
  ACGGGGAAAAAGTGCAGCTTGCCGGCCGAGGAGGATTCTGTGTTAGAAAAACTAGGGGAAAGGAAACCAT
  GCAACAGTCAACCTTCTGAGCTTTCTTCAGAGACCTCAGGAATAGCCAGGCCAGAAGAAGGAAGGCCTGT
  GGTGAGTGGGACAGGAAATGACATCACCACCCCACCGAACAAGGAGCTCCCACCAAGCCCAGAGAAGAAA
  ACAAAGCCTTTGGCCACCACTCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTC
  TCCCTAAGCAGCCAGCTCCCACCACCATTGGTGGGTTGAATAAAAAACCCATGAGCCTTGCTTCAGGCTT
  AGTGCCAGCTGCCCCACCCAAACGCCCTGCCGTCGCCTCTGCCAGGCCTTCCATCTTACCTTCAAAAGAC
  GTGAAGCCAAAGCCCATTGCAGATGCAAAGGCTCCTGAGAAGCGGGCCTCACCATCCAAGCCAGCTTCTG
  CCCCAGCCTCCAGATCTGGGTCCAAGAGCACTCAGACTGTTGCAAAAACCACAACAGCTGCTGCTGTTGC
  CTCAACTGGCCCAAGCAGTAGGAGCCCCTCCACGCTCCTGCCCAAGAAGCCCACTGCCATTAAGACTGAG
  GGAAAACCTGCAGAAGTCAAGAAGATGACTGCAAAGTCTGTACCAGCTGACTTGAGTCGCCCAAAGAGCA
  CCTCCACCAGTTCCATGAAGAAAACCACCACTCTCAGTGGGACAGCCCCCGCTGCAGGGGTGGTTCCCAG
  CCGAGTCAAGGCCACACCCATGCCCTCCCGGCCCTCCACAACTCCTTTCATAGACAAGAAGCCCACCTCG
  GCCAAACCCAGCTCCACCACCCCCCGGCTCAGCCGCCTGGCCACCAATACTTCTGCTCCTGATCTGAAGA
  ATGTCCGCTCCAAGGTTGGCTCCACGGAAAACATCAAGCATCAGCCTGGAGGAGGCCGGGCCAAAGTAGA
  GAAAAAAACAGAGGCAGCTGCTACAACCCGAAAGCCTGAATCTAATGCAGTCACTAAAACAGCCGGCCCA
  ATTGCAAGTGCACAGAAACAACCTGCGGGGAAAGTCCAGATAGTCTCCAAAAAAGTGAGCTACAGCCATA
  TTCAGTCCAAGTGTGGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGTAATGTTCAGATTCAGAA
  CAAGAAAGTGGACATCTCTAAGGTCTCCTCCAAGTGTGGGTCTAAGGCTAACATCAAGCACAAGCCTGGT
  GGAGGAGATGTCAAGATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAGGCCAAGGTGGGATCCC
  TCGATAATGTGGGCCACCTACCTGCAGGAGGTGCTGTGAAGACTGAGGGCGGTGGCAGCGAGGCTCCTCT
  GTGTCCGGGTCCCCCTGCTGGGGAGGAGCCGGCCATCTCTGAGGCAGCGCCTGAAGCTGGCGCCCCCACT
  TCAGCCAGTGGCCTCAATGGCCACCCCACCCTGTCAGGGGGTGGTGACCAAAGGGAGGCCCAGACCTTGG
  ACAGCCAGATCCAGGAGACAAGCATCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002375 unedited
  AGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCAGCTCC
  GTCTCGGCGGCGGCGGGCAGTTGCAGTGGTGCAGAATGGCTGACCTCAGTCTTGCAGATG
  CATTAACAGAACCATCTCCAGACATTGAGGGAGAGATAAAGCGGGACTTCATTGCCACAC
  TAGAGGCAGAGGCCTTTGATGATGTTGTGGGAGAAACTGTTGGAAAAACAGACTATATTC
  CTCTCCTGGATGTTGATGAGAAAACCGGGAACTCAGAGTCAAAGAAGAAACCGTGCTCAG
  AAACTAGCCAGATTGAAGATACTCCATCTTCTAAACCAACACTCCTAGCCAATGGTGGTC
  ATGGAGTAGAAGGGAGCGATACTACAGGGTCTCCAACTGAATTCCTTGAAGAGAAAATGG
  CCTACCAGGAATACCCAAATAGCCAGAACTGGCCAGAAGATACCAACTTTTGTTTCCAAC
  CTGAGCAAGTGGTCGATCCTATCCAGACTGATCCCTTTAAGATGTACCATGATGATGACC
  TGGCAGATTTGGTCTTTCCCTCCAGTGCGACAGCTGATACTTCAATATTTGCAGGACANA
  ATGATCCCTTGAAAGACAGTTACGGTATGTCTCCCTGCAACACAGCTGTTGTACCTCAGG
  NGTGGCCTGTGGAAGCCTTAACTCTNCACACTCAGAGTCCTTTGTTTCCCCAGAGGGCTG
  TGCAGAACCTNCTCAGCCAACGGCAGTTCCCTTAGAGCTANGCCAGGAGATAGAAATGCC
  ATCAGAAGAAGAGGCACCAGCANCAGCATTGGNNAATATGATGGGACTGAAGACTACTGA
  CATGGCACCATCTAAAGAAACAGAGATGCCCTCCGCCAGGACATGCACTACCTACAAAAA
  CCGAGGTGGCATTGGCTCAAGATATGGATCCACCCACCAATTAGATGTGACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002375 unedited
  CTATGGACCGCGGCACGCTTCTATGATCGAGTTTTTTTTTTTTTTTTTTGGGGACATCAG
  GCAGCTTTATTTATTTACTCTTAAAACTGTTACAACAGAATCATGGACTGACACAGGTAA
  TGGCTGAGCCATAAGCAAATCGAGAAGTACAGAAATGTCCCACCCCAAACAGCTGCGGAG
  TACACATCACACAGGGCCTCTGGTCCCGGCCTTCTCAAGTGCTCTGGAGTGGAGGATCCT
  TTGAGGGAACTCTGACCACTCCTGTTGTCTACCTAAAGAGCACGCCACTTGGGCCACCTA
  CCCCCAACCTTTGGCCAAAGGAGTGAAAGGACCTGGAACCTGTCGTCAACCTCAGCATCC
  TCATGACCCCAGGACAGACGCAGTGGAGGCAGCAGGTACTCTGGGCCTGTGCTGTTGCCC
  CTGAGGTGTCTCCTGACTACGCAACCCTGTTTCCTTCCCACTTTCATCCCAGCAGGCCCT
  TTCTTCCTGCTGACAAAACACTGGCTCCCTCATGTTCTTGGCACAGCAGAAGTGTTCTGC
  ACATGGTCTCTACGGAGGAAAGCTGGTCCCCCTGGCCCAGGCAGCACTTCCTCCGTGAGC
  CCCTGCAGTTTCCTGGTGTGTGCTTGGGGAGCTAGGGACATGGTGTCAAACCTTCACAAA
  GCACTGCTACCTCAGATAGGGGGAGGACCTGTCAAGCCCAGGACAGCCTCCCTGCACATG
  GAGATCAAATCTAAACTTAAGCAAGACCCCTACCTTCTCCAGGGATGCCAGGACCANAGA
  ATGGCANAGGTCATGAAGGGGCATGGATGAGCCCAGTCTGGGGCACCCCTCTAAAAGGCA
  ATCTACACTGCAGCCAATCCCCCTCACGAAAGCCCTTGGATCTCCAAGCCTTNCACTAAA
  ACTTAGTTGAATCCTTTTTAAAAACCTGCAACTTAATACAAAAAACAAGGCCAAAGAACC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002375
• Insert Size: 5850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002375.3, NP_002366.2
• RefSeq Size: 6337 bp
• RefSeq ORF: 3459 bp
• Locus ID: 4134
• Cytogenetics: 3p21.31
• Domains: tubulin-binding
• Gene Summary: 'The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. It encodes the longest isoform (1).