Tau (MAPT) (NM_016841) Human Untagged Clone

CAT#: SC109404

MAPT (untagged)-Human microtubule-associated protein tau (MAPT), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPT
• Synonyms: DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109404 sequence for NM_016841 edited (data generated by NextGen Sequencing)
  ATGGCTGAGCCCCGCCAGGAGTTCGAAGTGATGGAAGATCACGCTGGGACGTACGGGTTG
  GGGGACAGGAAAGATCAGGGGGGCTACACCATGCACCAAGACCAAGAGGGTGACACGGAC
  GCTGGCCTGAAAGCTGAAGAAGCAGGCATTGGAGACACCCCCAGCCTGGAAGACGAAGCT
  GCTGGTCACGTGACCCAAGCTCGCATGGTCAGTAAAAGCAAAGACGGGACTGGAAGCGAT
  GACAAAAAAGCCAAGGGGGCTGATGGTAAAACGAAGATCGCCACACCGCGGGGAGCAGCC
  CCTCCAGGCCAGAAGGGCCAGGCCAACGCCACCAGGATTCCAGCAAAAACCCCGCCCGCT
  CCAAAGACACCACCCAGCTCTGGTGAACCTCCAAAATCAGGGGATCGCAGCGGCTACAGC
  AGCCCCGGCTCCCCAGGCACTCCCGGCAGCCGCTCCCGCACCCCGTCCCTTCCAACCCCA
  CCCACCCGGGAGCCCAAGAAGGTGGCAGTGGTCCGTACTCCACCCAAGTCGCCGTCTTCC
  GCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGACCTGAAGAATGTCAAGTCC
  AAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGGGAGGCGGGAAGGTGCAAATAGTC
  TACAAACCAGTTGACCTGAGCAAGGTGACCTCCAAGTGTGGCTCATTAGGCAACATCCAT
  CATAAACCAGGAGGTGGCCAGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGA
  GTCCAGTCGAAGATTGGGTCCCTGGACAATATCACCCACGTCCCTGGCGGAGGAAATAAA
  AAGATTGAAACCCACAAGCTGACCTTCCGCGAGAACGCCAAAGCCAAGACAGACCACGGG
  GCGGAGATCGTGTACAAGTCGCCAGTGGTGTCTGGGGACACGTCTCCACGGCATCTCAGC
  AATGTCTCCTCCACCGGCAGCATCGACATGGTAGACTCGCCCCAGCTCGCCACGCTAGCT
  GACGAGGTGTCTGCCTCCCTGGCCAAGCAGGGTTTGTGA
  
  Clone variation with respect to NM_016841.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016841 unedited
  TTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAAGGGACCGCGAAGGGCAGCG
  CCGAGAGGAAACGAGCCGGGAGACGCCGGACGGCCGAGCGGCAGGGCGCTCGCGCGCGCC
  CACTAGTGGCCGGAGGAGAAGGCTCCCGCGGAGGCCGCGCTGCCCGCCCCCTCCCCTGGG
  GAGGCTCGCGTTCCCGCTGCTCGCGCCTGCGCCGCCCGCCGGCCTCAGGAACGCGCCCTC
  TTCGCCGGCGCGCGCCCTCGCAGTCACCGCCACCCACCAGCTCCGGCACCAACAGCAGCG
  CCGCTGCCACCGCCCACCTTCTGCCGCCGCCACCACAGCCACCTTCTCCTCCTCCGCTGT
  CCTCTCCCGTCCTCGCCTCTGTCGACTATCAGGTGAACTTTGAACCAGGATGGCTGAGCC
  CCGCCAGGAGTTCGAAGTGATGGAAGATCACGCTGGGACGTACGGGTTTGGGGGACAGGA
  AAGATCAAGGGGGCTACACCATGCACCAAGACCAAGAGGGTGACACGGACGCTGGCCTGA
  AAGCTGAAGAAGCAGGCATTGGAGACACCCCCAGCCTGGAAGACGAAGCTGCTGGTCACG
  TGACCCAAGCTCGCATGGTCAGTAAAAGCAAAGACGGGACTGGAAAGCGATGACAAAAAA
  GCCAAGGGGCCTGATGGTAAAACGAAGATCGCCACACCGCGGGGGAGCAGCCCCTCCAGC
  CCAGAAGGGGCCAGGCCAACGCCACCCAGATTCCAGCANAAACCCCGCCCGCTCCAAAGA
  ACACCACCAGCTTCTGGTGAACCTCCAAATCCANGGGGATCGCCAGCGGCTACAGCAGCC
  CCGGCTCCCCCAGCACTCCCGGGAGCCGTTTCCCGAACCCCGTCCCTTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016841 unedited
  NTATTACTNAGNAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTATTA
  CTAGCCCACCCATCAATTTGNGAAAGATGAAATTTGCTCTTACTCCCATCACTGATTTTG
  AAGTCCCGAGCCAAAGCCGAGTGACAAAAGCAGGTTAAGTGATTAACCAATTAACCGAAC
  TGCGAGGAGCAGCTGGGGGCAGAGGGCGGGGGCCGGGTCATTATTCTTTTTTTTTCCACA
  CTCTCTCATTCTCTCCTCTCCACAATTATTGACCGCCCCAGGGGCCTGATCACAAACCCT
  GCTTGGCCAGGGAGGCAGACACCTCGTCAGCTAGCGTGGCGAGCTGGGGCGAGTCTACCA
  TGTCGATGCTGCCGGTGGAGGAGACATTGCTGAGATGCCGTGGAGACGTGTCCCCAGACA
  CCACTGGCGACTTGTACACGATCTCCGCCCCGTGGTCTGTCTTGGCTTTGGCGTTCTCGC
  GGAAGGTCAGCTTGTGGGTTTCAATCTTTTTATTTCCTCCGCCAGGGACGTGGGTGATAT
  TGTCCAGGGACCCAATCTTCGACTGGACTCTGTCCTTGAAGTCAAGCTTCTCAGATTTTA
  CTTCCACCTGGCCACCTCCTGGTTTATGATGGATGTTGCCTAATGAGCCACACTTGGAGG
  TCACCTTGCTCAAGTCAACTGGTTTGTAGACTATTTGCACCTTCCCGCCCTCCGGCTGGT
  GCCTTCAGTTCTCAGTGGAGCCGATCCTGGACTTTGACATTCTTAGGTCTGGCAGGGGCA
  CGGGGGCTGTCTGGCAGGCGCTCTTGGGGGAAAACGGCCACCTGGGTGGAGTTCGGACCA
  CTGGCCACCTCTTGGGGCCCCGGTGGGTGGGGTTTGAAAAGCACCGTTTCCCCTATATAC
  CTGCGCGGTAGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016841
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016841.1, NP_058525.1
• RefSeq Size: 2529 bp
• RefSeq ORF: 1059 bp
• Locus ID: 4137
• Cytogenetics: 17q21.31
• Domains: tubulin-binding
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, MAPK signaling pathway
• Gene Summary: 'This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) lacks six internal coding exons, as compared to variant 6. The reading frame is not affected, and the resulting isoform (4) has identical N- and C-termini but lacks five segments, as compared to isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.