CD10 (MME) (NM_000902) Human Untagged Clone

CAT#: SC109440

MME (untagged)-Human membrane metallo-endopeptidase (MME), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MME
• Synonyms: CALLA; CD10; CMT2T; NEP; SCA43; SFE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109440 sequence for NM_000902 edited (data generated by NextGen Sequencing)
  ATGGGCAAGTCAGAAAGTCAGATGGATATAACTGATATCAACACTCCAAAGCCAAAGAAG
  AAACAGCGATGGACTCCACTGGAGATCAGCCTCTCGGTCCTTGTCCTGCTCCTCACCATC
  ATAGCTGTGACAATGATCGCACTCTATGCAACCTACGATGATGGTATTTGCAAGTCATCA
  GACTGCATAAAATCAGCTGCTCGACTGATCCAAAACATGGATGCCACCACTGAGCCTTGT
  ACAGACTTTTTCAAATATGCTTGCGGAGGCTGGTTGAAACGTAATGTCATTCCCGAGACC
  AGCTCCCGTTACGGCAACTTTGACATTTTAAGAGATGAACTAGAAGTCGTTTTGAAAGAT
  GTCCTTCAAGAACCCAAAACTGAAGATATAGTAGCAGTGCAGAAAGCAAAAGCATTGTAC
  AGGTCTTGTATAAATGAATCTGCTATTGATAGCAGAGGTGGAGAACCTCTACTCAAACTG
  TTACCAGACATATATGGGTGGCCAGTAGCAACAGAAAACTGGGAGCAAAAATATGGTGCT
  TCTTGGACAGCTGAAAAAGCTATTGCACAACTGAATTCTAAATATGGGAAAAAAGTCCTT
  ATTAATTTGTTTGTTGGCACTGATGATAAGAATTCTGTGAATCATGTAATTCATATTGAC
  CAACCTCGACTTGGCCTCCCTTCTAGAGATTACTATGAATGCACTGGAATCTATAAAGAG
  GCTTGTACAGCATATGTGGATTTTATGATTTCTGTGGCCAGATTGATTCGTCAGGAAGAA
  AGATTGCCCATCGATGAAAACCAGCTTGCTTTGGAAATGAATAAAGTTATGGAATTGGAA
  AAAGAAATTGCCAATGCTACGGCTAAACCTGAAGATCGAAATGATCCAATGCTTCTGTAT
  AACAAGATGACATTGGCCCAGATCCAAAATAACTTTTCACTAGAGATCAATGGGAAGCCA
  TTCAGCTGGTTGAATTTCACAAATGAAATCATGTCAACTGTGAATATTAGTATTACAAAT
  GAGGAAGATGTGGTTGTTTATGCTCCAGAATATTTAACCAAACTTAAGCCCATTCTTACC
  AAATATTCTGCCAGAGATCTTCAAAATTTAATGTCCTGGAGATTCATAATGGATCTTGTA
  AGCAGCCTCAGCCGAACCTACAAGGAGTCCAGAAATGCTTTCCGCAAGGCCCTTTATGGT
  ACAACCTCAGAAACAGCAACTTGGAGACGTTGTGCAAACTATGTCAATGGGAATATGGAA
  AATGCTGTGGGGAGGCTTTATGTGGAAGCAGCATTTGCTGGAGAGAGTAAACATGTGGTC
  GAGGATTTGATTGCACAGATCCGAGAAGTTTTTATTCAGACTTTAGATGACCTCACTTGG
  ATGGATGCCGAGACAAAAAAGAGAGCTGAAGAAAAGGCCTTAGCAATTAAAGAAAGGATC
  GGCTATCCTGATGACATTGTTTCAAATGATAACAAACTGAATAATGAGTACCTCGAGTTG
  AACTACAAAGAAGATGAATACTTCGAGAACATAATTCAAAATTTGAAATTCAGCCAAAGT
  AAACAACTGAAGAAGCTCCGAGAAAAGGTGGACAAAGATGAGTGGATAAGTGGAGCAGCT
  GTAGTCAATGCATTTTACTCTTCAGGAAGAAATCAGATAGTCTTCCCAGCCGGCATTCTG
  CAGCCCCCCTTCTTTAGTGCCCAGCAGTCCAACTCATTGAACTATGGGGGCATCGGCATG
  GTCATAGGACACGAAATCACCCATGGCTTCGATGACAATGGCAGAAACTTTAACAAAGAT
  GGAGACCTCGTTGACTGGTGGACTCAACAGTCTGCAAGTAACTTTAAGGAGCAATCCCAG
  TGCATGGTGTATCAGTATGGAAACTTTTCCTGGGACCTGGCAGGTGGACAGCACCTTAAT
  GGAATTAATACACTGGGAGAAAACATTGCTGATAATGGAGGTCTTGGTCAAGCATACAGA
  GCCTATCAGAATTATATTAAAAAGAATGGCGAAGAAAAATTACTTCCTGGACTTGACCTA
  AATCACAAACAACTATTTTTCTTGAACTTTGCACAGGTGTGGTGTGGAACCTATAGGCCA
  GAGTATGCGGTTAACTCCATTAAAACAGATGTGCACAGTCCAGGCAATTTCAGGATTATT
  GGGACTTTGCAGAACTCTGCAGAGTTTTCAGAAGCCTTTCACTGCCGCAAGAATTCATAC
  ATGAATCCAGAAAAGAAGTGCCGGGTTTGGTGA
  
  Clone variation with respect to NM_000902.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000902 unedited
  GGTGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGCAGC
  AGCCTCCAACTTCTCCCGAATCCCACTGATTTTAGGTGATGGGCAAGTCAGAAAGTCAGA
  TGGATATAACTGATATCAACACTCCAAAGCCAAAGAAGAAACAGCGATGGACTCCACTGG
  AGATCAGCCTCTCGGTCCTTGTCCTGCTCCTCACCATCATAGCTGTGACAATGATCGCAC
  TCTATGCAACCTACGATGATGGTATTTGCAAGTCATCAGACTGCATAAAATCAGCTGCTC
  GACTGATCCAAAACATGGATGCCACCACTGAGCCTTGTACAGACTTTTTCAAATATGCTT
  GCGGAGGCTGGTTGAAACGTAATGTCATTCCCGAGACCAGCTCCCGTTACGGCAACTTTG
  ACATTTTAAGAGATGAACTAGAAGTCGTTTTGAAAGATGTCCTTCAAGAACCCAAAACTG
  AAGATATAGTAGCAGTGCAGAAAGCAAAAGCATTGTACAGGTCTTGTATAAATGAATCTG
  CTATTGATAGCAGAGGTGGAGAACCTCTACTCAAACTGTTACCAGACATATATGGGTGGC
  CAGTAGCAACAGAAAACTGGGAGCAAAAATATGGTGCTTCTTGGACAGCTGAAAAAGCTA
  TTGCACAACTGAATTCTAAATATGGGAAAAAAGTCCTTATTAATTTGTTTGTTGGCACTG
  ATGATAAGAATTCTGTGAATCATGTAATTCATATTGACCAACCTCGACTTGGCCTCCCTT
  CTAGAGATACTATGAATGCACTGGAAATCTATAAGAGGCTTGTACAGCATATGTGGATTT
  TATGAATTCTGTGGCCAGAATGATTCGTCAGGAAGAAAGATTGCCATCGATGAAACCAGC
  TTGCTTTGGAATGAATAAAGTATGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000902 unedited
  CCCCATTTGTTGCCCGCCGGCCGCATTCTACATCGCGTTTTTTTTTTTTTTTTTTACCAT
  CACCAACGTTTGTTTTTATTTTGCATTTTTGAGACTGGAAACTGATAAACATTTCTGATT
  AGGAAACTTTGACAATACAGTCTTTCTTCTTTCCAAAATAACAAATGTCTTTTAGTTTGA
  GAGCTCCAGCTGTGGCAACAAGAATGATAAATGCTTTTGAGAAAATTAGCACGGTTTTGC
  CCCACTTAAATGGTTAACAGTAACAGTAGTACAGACCCAAACTTGACAAAACTTTGGCAC
  TTGTTATTTGGACTAAAATTTTGTCTCACTCCATTCACACACGTCCATGGGAGTTTTCAG
  TTTATCCTGCCCACTGATTCATACCTGGCAGACCGATTCCCTGGGACGGCAGCATTGGAC
  ACCTACTCCTTCTCTCGCTGACAACCACCAATATACTCTAATTCTCTTTTTCTAGGCGAG
  AGACGCCCCGGCCCACCGGAAAATTGCACACCCCCCAGGCCCCCCTCGCCCGAGCGCCAC
  ACCCCGCCCCCAACAGGCACTTTTTCCTTGCCCCGCTGCCCTTCCAATCCTTCCACTTCG
  CCCCTCCCTCCCACCAACACCGCTCCTCGCAGCTATCTTCCTCCCCACCCCCCCCTGCCC
  CCCGCCCCTTCTCCCCACCTCTCTCGCCACTTTCCTACCTCTCCAACCATTTTTGTCTAT
  AAAAATTTATCAATTCCCTCTCTTCCCCCCATACCCTCCTCCCTCTCACCCCTCGTCTCT
  ACTGCCCGTCACACACCCCAAACGCCCCCCCTGCCCGTCCCAACCCACCACGACGCCGCC
  CCCCCGCCTCACCCATCACACATAGTCATCCCACCCCATCCCCCCCTCAACTCCCTCCCC
  ACTCCCTCCCTCTCCGCCTCCTCCCCACCCGCTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000902
• Insert Size: 5850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000902.2, NP_000893.1
• RefSeq Size: 5595 bp
• RefSeq ORF: 2253 bp
• Locus ID: 4311
• Cytogenetics: 3q25.2
• Domains: Peptidase_M13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Hematopoietic cell lineage, Renin-angiotensin system
• Gene Summary: 'The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) contains an alternate 5' UTR exon compared to variant 2b. Variants 1, 1bis, 2a, 2b, 3, and 4 all encode the same isoform (a).