myosin light chain 1 (MYL1) (NM_079420) Human Untagged Clone

CAT#: SC109466

MYL1 (untagged)-Human myosin, light chain 1, alkali, skeletal, fast (MYL1), transcript variant 1f


  "NM_079420" in other vectors (4)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MYL1
Synonyms MLC1F; MLC3F; MYOFTA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_079420, the custom clone sequence may differ by one or more nucleotides


ATGGCACCAAAGAAAGACGTGAAGAAACCTGTGGCTGCGGCTGCGGCTGCCCCAGCCCCGGCACCGGCAC
CTGCACCTGCCCCTGCCCCAGCCAAACCCAAAGAAGAAAAAATTGACCTCTCTGCCATTAAGATCGAGTT
CTCTAAGGAACAGCAGGATGAATTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGTGATTCCAAGATC
ACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACAAATCCCACCAATGCAGAGGTCAGGAAAG
TTCTGGGAAACCCCAGCAATGAAGAGCTGAATGCCAAGAAAATTGAGTTTGAACAATTTCTGCCTATGAT
GCAAGCCATTTCCAACAACAAGGACCAGGCCACCTATGAAGACTTTGTTGAGGGTCTGCGTGTCTTTGAC
AAGGAAGGCAATGGCACAGTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGAAAAGATGA
AAGAGGAAGAAGTGGAAGCCCTGATGGCAGGTCAAGAAGACTCCAATGGCTGCATCAACTACGAAGCTTT
TGTCAAGCACATCATGTCTATCTGA


>OriGene 5' read for NM_079420 unedited
NTTCTTTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
AAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCC
GCGAATTCGGCACGAGGGAGCAGCCCTCAACTCACTCTTCAGCTTCCCTGCTGTGTTGCA
GCCCAGCCGCTCCATCATGCTTTTTATGTTCCTAGATTCAAAAGTATCCTAAGGCAACCT
TCTAATGCCAAGACACCTACATAGCTTCCTAAAAATCTCAGAGCAACCAGCACTCATTCT
TCTGTCAATGCATTTGAAAGAGTACTAGTTTTTTTTCTTTCTTTCTCTTCTTCGCCACTG
CAGTCCTTCAGTGCTGACCAGATTGCTGAATTCAAGGAGGCATTTCTCCTGTTTGACAGA
ACAGGTGATTCCAAGATCACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACA
AATCCCACCAATGCAGAGGTCAGGAAAGTTCTGGGAAACCCCAGCAATGAAGAGCTGAAT
GCCAAGAAAATTGAGTTTGAACAATTTCTGCCTATGATGCAAGCCATTTCCAANCACAGG
NACCAGGCCACCTATGAAGACTTTGTTGAGGGTCTGCGTGTCTTTGACAGGAANGGCATG
GCACAGTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGAAAAAGATGAAA
GAGAAGAAGTGGAAGCCCTGATGGCAGGTCAGAAGACTCAATGGCTGCATAACTACGAAG
CTTTGTCAAGCCATCATGTCTATCTGAATGGAGCTCCAAGAACCAGCATTGTTTAGGAGA
CTGGCTGGAAACTATTTTAATCACACCATGAAAAACTCTCCGATCTGTTTACCATCATTC
GGAC
Restriction Sites NotI-NotI     
ACCN NM_079420
Insert Size 1100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_079420.1, NP_524144.1
RefSeq Size 1052 bp
RefSeq ORF 585 bp
Locus ID 4632
Cytogenetics 2q34
Domains EFh
Gene Summary 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1f) encodes a protein that is 44 aa longer than isoform 3f. Transcript variants 1f and 3f differ in their first two exons.

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