myosin light chain 1 (MYL1) (NM_079420) Human Untagged Clone

CAT#: SC109466

MYL1 (untagged)-Human myosin, light chain 1, alkali, skeletal, fast (MYL1), transcript variant 1f

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL1
• Synonyms: MLC1F; MLC3F; MYOFTA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_079420, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCACCAAAGAAAGACGTGAAGAAACCTGTGGCTGCGGCTGCGGCTGCCCCAGCCCCGGCACCGGCAC
  CTGCACCTGCCCCTGCCCCAGCCAAACCCAAAGAAGAAAAAATTGACCTCTCTGCCATTAAGATCGAGTT
  CTCTAAGGAACAGCAGGATGAATTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGTGATTCCAAGATC
  ACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACAAATCCCACCAATGCAGAGGTCAGGAAAG
  TTCTGGGAAACCCCAGCAATGAAGAGCTGAATGCCAAGAAAATTGAGTTTGAACAATTTCTGCCTATGAT
  GCAAGCCATTTCCAACAACAAGGACCAGGCCACCTATGAAGACTTTGTTGAGGGTCTGCGTGTCTTTGAC
  AAGGAAGGCAATGGCACAGTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGAAAAGATGA
  AAGAGGAAGAAGTGGAAGCCCTGATGGCAGGTCAAGAAGACTCCAATGGCTGCATCAACTACGAAGCTTT
  TGTCAAGCACATCATGTCTATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_079420 unedited
  NTTCTTTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCC
  GCGAATTCGGCACGAGGGAGCAGCCCTCAACTCACTCTTCAGCTTCCCTGCTGTGTTGCA
  GCCCAGCCGCTCCATCATGCTTTTTATGTTCCTAGATTCAAAAGTATCCTAAGGCAACCT
  TCTAATGCCAAGACACCTACATAGCTTCCTAAAAATCTCAGAGCAACCAGCACTCATTCT
  TCTGTCAATGCATTTGAAAGAGTACTAGTTTTTTTTCTTTCTTTCTCTTCTTCGCCACTG
  CAGTCCTTCAGTGCTGACCAGATTGCTGAATTCAAGGAGGCATTTCTCCTGTTTGACAGA
  ACAGGTGATTCCAAGATCACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACA
  AATCCCACCAATGCAGAGGTCAGGAAAGTTCTGGGAAACCCCAGCAATGAAGAGCTGAAT
  GCCAAGAAAATTGAGTTTGAACAATTTCTGCCTATGATGCAAGCCATTTCCAANCACAGG
  NACCAGGCCACCTATGAAGACTTTGTTGAGGGTCTGCGTGTCTTTGACAGGAANGGCATG
  GCACAGTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGAAAAAGATGAAA
  GAGAAGAAGTGGAAGCCCTGATGGCAGGTCAGAAGACTCAATGGCTGCATAACTACGAAG
  CTTTGTCAAGCCATCATGTCTATCTGAATGGAGCTCCAAGAACCAGCATTGTTTAGGAGA
  CTGGCTGGAAACTATTTTAATCACACCATGAAAAACTCTCCGATCTGTTTACCATCATTC
  GGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_079420
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_079420.1, NP_524144.1
• RefSeq Size: 1052 bp
• RefSeq ORF: 585 bp
• Locus ID: 4632
• Cytogenetics: 2q34
• Domains: EFh
• Gene Summary: 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1f) encodes a protein that is 44 aa longer than isoform 3f. Transcript variants 1f and 3f differ in their first two exons.