Myosin Phosphatase 2 (PPP1R12B) (NM_032104) Human Untagged Clone

CAT#: SC109475

PPP1R12B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R12B
• Synonyms: MYPT2; PP1bp55
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109475 sequence for NM_032104 edited (data generated by NextGen Sequencing)
  ATGGACAAAAATGAGAATGAAGAAGCAGATTTGGATGAGCAGTCCTCTAAGAGGCTGTCC
  ATCCGAGAGAGGAGGCGGCCCAAGGAACGACGAAGAGGCACAGGCATCAATTTCTGGACA
  AAGGATGAGGATGAAACTGATGGCTCTGAAGAGGTCAAAGAAACGTGGCATGAAAGACTT
  TCTAGGTTGGAATCGGGAGGTAGTAATCCTACAACCAGTGATTCTTACGGTGACCGGGCT
  TCAGCAAGAGCCCGTCGGGAGGCCCGGGAGGCCCGCCTAGCCACCCTGACCAGCCGTGTA
  GAAGAAGACAGCAACAGAGATTATAAAAAACTCTATGAGAGTGCTCTGACTGAAAACCAA
  AAACTGAAAACAAAACTTCAGGAAGCCCAGCTAGAGCTAGCAGATATAAAGTCCAAGCTT
  GAGAAGGTGGCCCAGCAGAAACAAGAAAAGACCTCTGACCGATCATCAGTGCTGGAGATG
  GAGAAACGGGAGAGGCGAGCCTTGGAGCGCAAAATGTCAGAAATGGAGGAAGAAATGAAG
  AACCTCCACCAGCTAAAACAGATTCAAACCTTGAAGCAGATGAACGAGCAACTGCAGGCT
  GAGAACAGGGCCCTGACCCGAGTGGTGGCCAGACTCTCGGAGTCCATCGAGTCCTCGGAC
  ACCCAGGAGCTCTAG
  
  Clone variation with respect to NM_032104.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032104 unedited
  GTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGACAAATAGATTTTCAG
  TCCCTGATTCTGAGAGTTCAGAGACTACCACAAACACTACAACTGCAAAGGAAATGGACA
  AAAATGAGAATGAAGAAGCAGATTTGGATGAGCAGTCCTCTAAGAGGCTGTCCATCCGAG
  AGAGGAGGCGGCCCAAGGAACGACGAAGAGGCACAGGCATCAATTTCTGGACAAAGGATG
  AGGATGAAACTGATGGCTCTGAAGAGGTCAAAGAAACGTGGCATGAAAGACTTTCTAGGT
  TGGAATCGGGAGGTAGTAATCCTACAACCAGTGATTCTTACGGTGACCGGGCTTCAGCAA
  GAGCCCGTCGGGAGGCCCGGGAGGCCCGCCTAGCCACCCTGACCAGCCGTGTAGAAGAAG
  ACAGCAACAGAGATTATAAAAAACTCTATGAGAGTGCTCTGACTGAAAACCAAAAACTGA
  AAACAAAACTTCAGGAAGCCCAGCTAGAGCTAGCAGATATAAAGTCCAAGCTTGAGAAGG
  TGGCCCAGCAGAAACAAGAAAAGACCTCTGACCGATCATCAGTGCTGGAGATGGAGAAAC
  GGGAGAGGCGAGCCTTGGAGCGCAAAATGTCAGAAATGGAGGAAGAAATGAAGAACCTCC
  ACCAGCTAAAACAGATTCAAACCTTGAAGCAGATGAACGAGCAACTGCAGGCTGAGAACA
  GGGCCCTGACCCGAGTGGTGGCCAGACTCTCNGAGTCCATCGAGTCCTCGGACACCCAGG
  AGCTCTAGTTCTTGCCCCTACNTCTCACTCACTTCCCTCCTNCACTACTCCAGGTGGTAA
  CAGAAACTGAATCCGACACCAGAGCTGAANGAGAAAATGGTGCCCTCATCAGAGTATCAG
  CAACTGTCCAGTAGGCTAGGCTCAGATTATGAAGAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032104
• Insert Size: 3260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032104.1, NP_115287.1
• RefSeq Size: 2227 bp
• RefSeq ORF: 675 bp
• Locus ID: 4660
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 8. The encoded isoform (d, also known as sm-M20 or hHS-M21 B) is shorter than isoform h.