NASP (NM_152298) Human Untagged Clone

CAT#: SC109477

NASP (untagged)-Human nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NASP
• Synonyms: FLB7527; HMDRA1; PRO1999
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109477 sequence for NM_152298 edited (data generated by NextGen Sequencing)
  ATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAA
  ATTGAAGATGTTCCTGCTCCTTCTACATCTGCAGATAAAGTGGAGAGTCTGGATGTGGAT
  AGTGAAGCTAAGAAACTATTGGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCA
  GCAGCTGTCAATGCATTCCAGGAAGCAGCTAGTCTTTTAGGTAAGAAGTATGGAGAGACA
  GCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAATCACTTCTGGAGTTGGCAAGA
  ATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGAGGAAGAAGGA
  GAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGANNCTGAAGGC
  TCGGAAGAGGATGATAAAGAAAATGATAAGACTGAAGAAATGCCAAATGATTCAGTCCTT
  GAAAACAAGTCTCTTCAAGAAAATGAGGAGGAGGAGATTGGGAACCTAGAGCTTGCCTGG
  GATATGCTGGATTTAGCAAAGATCATTTTTAAAAGGCAAGAAACAAAAGAAGCACAGCTT
  TATGCTGCCCAGGCACATCTTAAACTCGGAGAAGTTAGTGTTGAATCTGAAAACTATGTG
  CAAGCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTGCAGGAACAGTACCTGGAAGCCCAC
  GACCGTCTCCTTGCAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTACAACTCTCAG
  TATGATGAGGCAGTGGCACAGTTCAGCAAATCTATTGAAGTCATTGAGAACAGAATGGCT
  GTACTAAACGAGCAGGTGAAGGAGGCTGAAGGATCGTCTGCTGAATACAAGAAAGAAATT
  GAGGAACTAAAGGAACTGCTACCCGAAATTAGAGAGAAGATAGAAGATGCAAAGGAGTCT
  CAGCGTAGTGGGAATGTAGCTGAACTGGCTCTGAAAGCTACTCTGGTGGAGAGTTCTACT
  TCAGGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTCTCCATGATTGCCAGTAGAAAGCCA
  ACAGACGGTGCTTCCTCATCAAATTGTGTGACTGATATTTCCCACCTTGTCAGAAAGAAG
  AGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCCAAACAAGAGCCG
  GAGGTGAACGGAGGCAGTGGGGATGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATG
  GAGGAGGAGGCTGAGAATCAGGCTGAAAGCCGGGCAGCAGTGGAGGGGACAGTGGAGGCT
  GGAGCTACAGTTGAAAGCACTGCATGTTAA
  
  Clone variation with respect to NM_152298.3
  411 a=>n;412 a=>n;423 a=>g;453 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152298 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCTGAGTGAGTCTCTG
  GCGTCCCAAATTGCCTGTTTTTCTCGCAGGCTCTATTCCGTTCGCTGGTTCGCCACCTCA
  GGGGAACGATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTG
  CCGACAAAATTGAAGATGTTCCTGCTCCTTCTACATCTGCAGATAAAGTGGAGAGTCTGG
  ATGTGGATAGTGAAGCTAAGAAACTATTGGGTTTAGGACAGAAACATCTGGTGATGGGGG
  ATATTCCAGCAGCTGTCAATGCATTCCAGGAAGCAGCTAGTCTTTTAGGTAAGAAGTATG
  GAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAATCACTTCTGGAGT
  TGGCAAGAATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGAGG
  AAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGAGG
  AAGCAAGGGAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGAAGCCA
  AAAAAACAGAAGACAAGTCTTTGGCAAAGCCTGAAACTGATAAAGAACAGGACAGTGAAA
  TGGAGAAGGGTGGAAGAGAAGATATGGATATAAGTAAATCTGCAGAGGAGCCACAGGAAA
  AAGTTGACTTGACTCTAGATTGGTTAACTGAAACCTCTGAAGAGGCAAAAGGAGGAGCAG
  CACCAGAAGGACCGAATGAAGCTGAGGTCACTTCTGGGAAGCCAGACAGGAAGTACCAGA
  TGCTGAGGAAGAAAAATCAGTTCTGGAAACTGATGTCAAAANAGTGCANAGAANAAGGAG
  GTCANGAGAGCAGGGAGAGGGTATTGTGAGCATAGAGGAGAAGCCAAAGAAGTTCAGAGA
  GCAGCCTGTGGTGACTCTTAAAGCAGGCACTGCANTGGAGGTAAANCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152298 unedited
  CGTTACTCTAAGAACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTGCTTACAATCTTTATTGAAGTTATACAAAAAAAATCCTCCCAAAGTGAAAAA
  ATACCTTATATACAAAAACACTTTCCCTTGGGAGGAGGGCTGTGCCCCCTCTTAACATGC
  AGTGCTTTCAACTGTAGCTCCAGCCTCCACTGTCCCCTCCACTGGTGCCCGGCTTTCAAC
  CTGATTCTCAACCTCCTCCTCCATGTTTTCCGAAACTTCATTTCCACTGGGGACAGCATC
  CCCACTGCCTCCGTTCACCTCCGGCTCTTGTTTGGCTTTCTTTGCATCATCTTTCCGGGG
  ACTCTCTTCCTCTGGTTTCCTCTTCTTTCTGACAAGGTGGGAAATATCAGTCACACAATT
  TGATGAAGAAACACCGTCTGTTGGCTTTCTACTGGCAATCATGGAGACTGAAGAACCTCC
  TCCACCAGGAGTGAAACCTGAAGTAGAACTCTCCACCAGAGTAGCTTTCAAAGCCAGTTC
  AACTACATTCCCACTACGCTGAGACTCCTTTGCATCTTCTATCTTCTCTCTAATTTCGGG
  GAGCAGTTCCTTTAGTTCCTCAATTTCTTTCTTGTATTCAGCAGACGATCCTTCAGCCTC
  CTTCACCTGCTCGTTTAGTACAGCCATTCTGTTCTCAATGACTTCAATAGATTTGCTGAA
  CTGTGCCACTGCCTCATCATACTGAGAGTTGTACCCATAAGCCAAGCCCAGCTGGTAGTG
  GGTCTCTGCAAAGAGACAGTCGTGGGCTTCCAGGTACTGTTCCTGCCAGTTAAAGCAAGA
  CTGGAACTCCTCCCAGCTTGCACATAGTTTTCAGATTCACACTAACCTCTCCGAGTTTAA
  GATGTGCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152298
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152298.1, NP_689511.1
• RefSeq Size: 2589 bp
• RefSeq ORF: 2367 bp
• Locus ID: 4678
• Cytogenetics: 1p34.1
• Domains: TPR
• Gene Summary: 'This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region, compared to variant 2. This results in a shorter protein (isoform 3), compared to isoform 2.