NBL1 (NM_005380) Human Untagged Clone

CAT#: SC109478

NBL1 (untagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NBL1
• Synonyms: D1S1733E; DAN; DAND1; NB; NO3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005380, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGCTTCGGGTCCTGGTGGGGGCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACA
  AGCTGGCACTGTTCCCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGCCACAG
  CGGCTGTGAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGCTACAGCGTCCCCAAC
  ACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGCATGCCAGCCCAGTCCATGTGGGAGA
  TTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTGCCCAGGGTGGACAAGCTGGTGGAGAAGATCCTGCA
  CTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGTCACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGAC
  GGGCCGGGATCCCAGCCCGGCACCCACCCTCACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTG
  AGCCCGAGGACCCCCCTGGGGCCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005380 unedited
  GCGAATTCGCACGAGGCGCAGCCCAGCCGAGCGTCGCGGGGCCGCCCCCCGCCCTGCCGG
  CCGCCTCGCCGAGCCTCCTGGGGCGCCCGGGCCCGCGACCCCCGCACCCAGCTCCGCAGG
  ACCGGCGGGCGCGCGCGGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTCCTGGTGGGG
  GCTGTCCTCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTGGCACTGTTC
  CCAGATAAGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGCCACAGCGGC
  TGTAAGGCCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGCTACAGCGTC
  CCCAACACCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGCATGCCAGCC
  CAGTCCATGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTGCCCAGGGTG
  GACAAGCTGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGT
  CACGAGGGGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCAGNCCGGACCAA
  ACCCTTAACCCCAATTCCCAACCCCATCCTGGCGGGCAGACCCCTGAGCCCCGAGACCCC
  CCCTGGGGCCCCCCAACAGAGAAAAAGGNGGGCTGAGACTGAAGCCCCCCCAATCTTCCT
  CCCTCTTATCCCCTGGGAATGGTGGGTCTCACTCTCTGGGGAAGCAGGGGAGAGCTGAAC
  CCCCCTTGGCACTGGAGGACTGGCTTCAACTCGACCTGATGCTGCCGGTTGCATGGAAAC
  TGAAGGGCCGGGTAGACCAACTGCCATTTATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005380 unedited
  NCCCTGGCGGGGNATATTTTCCTGGAGTAGTCTGNAGGGTCCANCAGGGNATACTGTCCA
  ATGGTGACAAGCTCCANAAGCCCGCGTCGCAACAGCCAGGAGGGCCAGGCCACCCCAGGC
  AGGAGGCAGTGGGCTGGCAGCCACCCTGGGCACAGAAGAGCAGACGCAGACAGTGCTGGG
  CAACGAGGGGCTTTCTTCATGGGCCCGCCTGCCCTGTCCCTCCCCCCAGGTCCCCACCTT
  CTAGGGTTAAAGTGCAGCTGGGAGGGAGGAGGCAGGCAGAATTGGGGAGCTAGAGAGAGC
  CCAAGTGAACCCTGACTGTCCACGCAAGTCCCATGTCCTCCTCGTCCTGGAGTTCCTCGA
  GGTTCAGCGAGCCCATCCCGCCTAGGGCCTCTGGAACCTTGGGGCGAGGGAGGTAACCCC
  TCACTCCCACACCCATCCGATATTTACAAAGAGCATCCCTGGGAGAGGGGAGAAGGCGGC
  CGGCTCTGGCAGCTTTCTGGGGGGCCTAGAACTTGGGGCACAGTGCGGGGCACTAACATT
  CCTAACGGGAGAGGAGGGGGATGCTCGTCTTCAGGGATGGTCTTTGGGGGTTGTCCCCCT
  CCTTCTGGCCTTGGAAGACTCTTCCCTTTTCCTTTTCCCTTCTTCCCTTTCCCCACCCAG
  CAAGGCAAGGAAGCCCAGAAAAGGCCCGGAGAGCCTGGCTCCCAACTTCTGCGCCACGAT
  TCACAGGGTCCTGCGCATCCCTGATTTCCAAACCCTTTTCTTTGGGGAAGGCTCCTTTAT
  ATGCCCCGGGGGCCAACTCTTCTTGGAAATGTTAAATGCGTTAACAGCCTTTGGGTTAAC
  TGCGTGTTCCGCACCCTCCTGTCNTCCGCAGGGCGCTTCCTATCCACTCCTCTCCTTTCC
  CTCGGGTTTGACTGATTCTTACTTAATCCCTCCACTTCACCGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005380
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005380.3, NP_005371.1
• RefSeq Size: 2184 bp
• RefSeq ORF: 2184 bp
• Locus ID: 4681
• Cytogenetics: 1p36.13
• Domains: DAN, CT
• Protein Families: Secreted Protein
• Gene Summary: 'This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, 4, 6, 7, and 8 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.