NBL1 (NM_005380) Human Untagged Clone

CAT#: SC322394

NBL1 (untagged)-Human neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 2


  "NM_005380" in other vectors (5)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NBL1
Synonyms D1S1733E; DAN; DAND1; NB; NO3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322394 GCCGAGCCTCCTGGGGCGCCCGGGCCCGCGACCCCCGCACCCAGCTCCGCAGGACCGGCG
GGCGCGCGCGGGCTCTGGAGGCCACGGGCATGATGCTTCGGGTCCTGGTGGGGGCTGTCC
TCCCTGCCATGCTACTGGCTGCCCCACCACCCATCAACAAGCTGGCACTGTTCCCAGATA
AGAGTGCCTGGTGCGAAGCCAAGAACATCACCCAGATCGTGGGCCACAGCGGCTGTGAGG
CCAAGTCCATCCAGAACAGGGCGTGCCTAGGACAGTGCTTCAGCTACAGCGTCCCCAACA
CCTTCCCACAGTCCACAGAGTCCCTGGTTCACTGTGACTCCTGCATGCCAGCCCAGTCCA
TGTGGGAGATTGTGACGCTGGAGTGCCCGGGCCACGAGGAGGTGCCCAGGGTGGACAAGC
TGGTGGAGAAGATCCTGCACTGTAGCTGCCAGGCCTGCGGCAAGGAGCCTAGTCACGAGG
GGCTGAGCGTCTATGTGCAGGGCGAGGACGGGCCGGGATCCCAGCCCGGCACCCACCCTC
ACCCCCATCCCCACCCCCATCCTGGCGGGCAGACCCCTGAGCCCGAGGACCCCCCTGGGG
CCCCCCACACAGAGGAAGAGGGGGCTGAGGACTGAGGCCCCCCCAACTCTTCCTCCCCTC
TCATCCCCCTGTGGAATGTTGGGTCTCACTCTCTGGGGAAGTCAGGGGAGAAGCTGAAGC
CCCCCTTTGGCACTGGATGGACTTGGCTTCAGACTCGGACTTGAATGCTGCCCGGTTGCC
ATGGAGATCTGAAGGGGCGGGGTTAGAGCCAAGCTGCACAATTTAATATATTCAAGAGTG
GGGGGAGGAAGCAGAGGTCTTCAGGGCTCTTTTTTTGGGGGGGGGGTGGTCTCTTCCTGT
CTGGCTTCTAGAGATGTGCCTGTGGGAGGGGGAGGAAGTTGGCTGAGCCATTGAGTGCTG
GGGGAGGCCATCCAAGATGGCATGAATCGGGCTAAGGTCCCTGGGGGTGCAGATGGTACT
GCTGAGGTCCCGGGCTTAGTGTGAGCATCTTGCCAGCCTCAGGCTTGAGGGAGGGCTGGG
CTAGAAAGACCACTGGCAGAAACAGGAGGCTCCGGCCCCACAGGTTTCCCCAAGGCCTCT
CACCCCACTTCCCATCTCCAGGGAAGCGTCGCCCCAGTGGCACTGAAGTGGCCCTCCCTC
AGCGGAGGGGTTTGGGAGTCAGGCCTGGGCAGGACCCTGCTGACTCGTGGCGCGGGAGCT
GGGAGCCAGGCTCTCCGGGCCTTTCTCTGGCTTCCTTGGCTTGCCTGGTGGGGGAAGGGG
AGGAGGGGAAGAAGGAAAGGGAAGAGTCTTCCAAGGCCAGAAGGAGGGGGACAACCCCCC
AAGACCATCCCTGAAGACGAGCATCCCCCTCCTCTCCCTGTTAGAAATGTTAGTGCCCCG
CACTGTGCCCCAAGTTCTAGGCCCCCCAGAAAGCTGTCAGAGCCGGCCGCCTTCTCCCCT
CTCCCAGGGATGCTCTTTGTAAATATCGGATGGGTGTGGGAGTGAGGGGTTACCTCCCTC
GCCCCAAGGTTCCAGAGGCCCTAGGCGGGATGGGCTCGCTGAACCTCGAGGAACTCCAGG
ACGAGGAGGACATGGGACTTGCGTGGACAGTCAGGGTTCACTTGGGCTCTCTCTAGCTCC
CCAATTCTGCCTGCCTCCTCCCTCCCAGCTGCACTTTAACCCTAGAAGGTGGGGACCTGG
GGGGAGGGACAGGGCAGGCGGGCCCATGAAGAAAGCCCCTCGTTGCCCAGCACTGTCTGC
GTCTGCTCTTCTGTGCCCAGGGTGGCTGCCAGCCCACTGCCTCCTGCCTGGGGTGGCCTG
GCCCTCCTGGCTGTTGCGACGCGGGCTTCTGGAGCTTGTCACCATTGGACAGTCTCCCTG
ATGGACCCTCAGTCTTCTCATGAATAAATTCCTTCAACGCCAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_005380
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005380.4, NP_005371.1
RefSeq Size 2021 bp
RefSeq ORF 543 bp
Locus ID 4681
Cytogenetics 1p36.13
Domains DAN, CT
Protein Families Secreted Protein
Gene Summary 'This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]'
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, 4, 6, 7, and 8 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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