INPP5F (OCRL) (NM_000276) Human Untagged Clone

CAT#: SC109507

OCRL (untagged)-Human oculocerebrorenal syndrome of Lowe (OCRL), transcript variant a


  "NM_000276" in other vectors (6)

Reconstitution Protocol

USD 1,820.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol OCRL
Synonyms Dent-2; INPP5F; LOCR; NPHL2; OCRL-1; OCRL1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000276 edited
ATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCTTGCCACTGTCGAGGGTATGGAGATG
AAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCCCAGAGGAACGGGCAATATGAG
TTAATAATCCAGTTGCATGAGAAGGAACAGCATGTTCAAGATATCATTCCTATAAATAGC
CACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTGATTGACATAGCTTCTAACAGT
GGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAGCGCCGCTTTGAAATCCCTGAT
GAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCTTGCTGCTCAGAAAGCTCAGTCACAG
CTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTACCAGAAATTAGACACTAAGGAC
AAACCTTCTGTTTTTTCAGGGCTTCTTGGATTTGAAGACAATTTTTCTTCTATGAATTTG
GACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATTCATCGGGAACCCCCACCTCCA
CCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAAGCTTCTAACAAGGAGCAGCCC
AAAGTGACCAACACCATGCGGAAGCTCTTTGTACCAAATACCCAATCTGGGCAGCGGGAG
GGTCTCATCAAACATATCCTGGCAAAGCGAGAGAAAGAATATGTCAACATTCAGACTTTC
AGATTTTTTGTTGGAACTTGGAATGTGAATGGCCAGTCTCCAGATAGCGGGTTAGAACCT
TGGCTGAACTGTGATCCCAATCCTCCTGATATCTACTGCATTGGATTCCAAGAACTGGAC
TTGAGCACAGAAGCCTTCTTCTACTTTGAATCTGTGAAGGAACAAGAATGGTCCATGGCT
GTAGAGAGAGGTTTGCATTCCAAAGCCAAGTATAAGAAAGTTCAACTGGTGCGCCTTGTT
GGGATGATGCTTCTTATATTTGCCAGAAAGGATCAGTGTCGATACATTCGTGATATTGCT
ACAGAAACAGTTGGAACTGGAATCATGGGGAAAATGGGAAACAAAGGTGGGGTAGCTGTG
AGATTTGTATTTCACAACACCACCTTTTGCATTGTCAATTCCCATCTGGCTGCACACGTG
GAGGACTTTGAGAGAAGGAATCAAGATTATAAGGACATTTGTGCGAGAATGAGTTTTGTG
GTCCCAAATCAGACCCTCCCGCAGTTGAACATCATGAAACATGAGGTTGTCATTTGGTTG
GGAGATTTGAATTATAGACTTTGCATGCCTGATGCCAATGAGGTGAAAAGTCTTATTAAT
AAGAAAGACCTTCAGAGACTCTTGAAATTCGACCAGCTAAATATTCAGCGCACACAGAAA
AAAGCTTTTGTTGACTTCAATGAAGGGGAAATCAAGTTCATCCCCACTTATAAGTATGAC
TCTAAAACAGACCGGTGGGATTCCAGTGGGAAATGCCGGGTTCCAGCCTGGTGTGACCGA
ATTCTTTGGAGAGGAACAAATGTTAATCAGCTTAATTATCGGAGTCACATGGAACTGAAA
ACCAGCGACCACAAGCCTGTTAGCGCCCTCTTCCATATTGGGGTGAAGGTTGTGGATGAA
CGAAGGTACCGGAAAGTCTTTGAAGATAGTGTACGCATCATGGACAGAATGGAAAATGAC
TTCCTTCCTTCCTTAGAACTCAGCAGGAGGGAGTTTGTGTTTGAAAATGTGAAGTTTCGG
CAACTACAAAAGGAGAAGTTCCAGATCAGCAACAATGGACAGGTTCCCTGCCATTTTTCT
TTCATCCCTAAACTTAATGACAGCCAGTACTGCAAGCCATGGCTTCGGGCTGAACCTTTT
GAGGGCTACTTGGAGCCAAATGAGACAGTGGACATTTCTCTTGATGTGTATGTCAGCAAA
GACTCTGTAACCATCCTGAACTCGGGAGAAGATAAGATTGAAGATATTCTCGTCCTTCAC
CTGGATCGAGGCAAAGATTACTTCTTGACTATCAGTGGAAATTACCTCCCAAGTTGTTTT
GGCACATCCTTAGAGGCTCTGTGCCGTATGAAAAGACCAATCCGAGAAGTTCCTGTTACC
AAACTCATAGACTTGGAAGAAGACAGCTTCCTAGAAAAGGAGAAATCCCTTCTGCAAATG
GTTCCTTTGGATGAAGGTGCCAGTGAGAGACCCCTTCAGGTTCCCAAGGAGATCTGGCTT
CTAGTAGATCACCTATTCAAATACGCCTGTCACCAGGAGGACCTGTTCCAGACCCCTGGA
ATGCAGGAAGAGCTCCAGCAGATCATTGATTGTCTGGATACCAGCATTCCTGAGACAATC
CCTGGCAGCAACCACTCTGTGGCTGAAGCACTGCTCATTTTCTTGGAAGCCCTGCCAGAG
CCAGTCATCTGTTACGAGCTGTATCAGCGATGTCTTGACTCTGCTTATGATCCCCGGATC
TGCCGACAGGTGATCTCCCAGCTTCCGAGATGCCATAGAAATGTTTTCCGTTACTTGATG
GCATTCCTTCGAGAACTCTTAAAATTCTCTGAATACAATAGCGTCAATGCCAACATGATC
GCTACTCTCTTCACTAGTCTTCTCCTGAGGCCTCCACCCAACCTTATGGCAAGACAGACT
CCAAGTGACCGCCAGCGTGCTATTCAGTTCCTTCTGGGCTTTCTGCTTGGGAGCGAAGAA
GACTAA
>OriGene 5' read for NM_000276 unedited
CGAGGCCGCTCCCGGCTCCCGGCGCCCGGCGCCCGGCGCGGAGCTGTTCCTCAAACGACA
CGCAGCCGAGGTGGGTGGGTGTGGGGACGCGGGAGCCAGTGTCGTCGGATCGGCCCGCAG
TCCGCTGTCCTGCTGAGCCCGGAGGCCGCCTGGATGGAGCCGCCGCTCCCGGTCGGAGCC
CAGCCGCTTGCCACTGTCGAGGGTATGGAGATGAAGGGTCCTCTCCGGGAGCCCTGCGCC
CTGACCCTAGCCCAGAGGAACGGGCAATATGAGTTAATAATCCAGTTGCATGAGAAGGAA
CAGCATGTTCAAGATATCATTCCTATAAATAGCCACTTCAGATGTGTTCAAGAAGCAGAA
GAAACTCTTTTGATTGACATAGCTTCTAACAGTGGCTGCAAAATTCGGGTTCAGGGGGAC
TGGATCAGAGAGCGCCGCTTTGAAATCCCTGATGAGGAACACTGTTTGAAGTTCCCTCTC
AGCTGTCCTTGCTGCTCAGAAAGCTCAGTCACAGCTTCTTGTTCCAGAGCANAAGGACTC
ATCTAGCTGGTACCAGAAATTAGACACTAAGGACANACCTTCTGTTTTTTCAGGGCTTCT
TGGATTTGAAGACAATTTTTCTTCTATGAATTTGGACAAGAAAATAAATTCACAAAATCA
GCCTACTGGGATTTCATCGGGAACCCCACCTCCCACCCTTTTCAGTGAATTAAAATGCCT
TCACCGTGAAAAAGAAACTTCTAACAAAGAGCAGCCCCAAGTGACCACCACCTGCCGAAG
CTCCNTGTACCAAATCCCAATCTGGGCAGCGGAAGTTCCCATTAAACTATTCTGGCCAAG
CGAGAAAAGATTTGTCC
>OriGene 3' read for NM_000276 unedited
ACTATGGACGCGGCCGCAATCTAGAATTTATTCANAAAAAATTTGCAATAAATTATAATA
AAAAAGAGACACGAAATATAGAATCCCACTAGCAGCTTTGCTTAGTGATCAAGACGTTTT
TGGGAAATATAACAAACAATTACAGAATAAGAAAACAGTGAAAGGGAGACTAATGCAATG
TCACCACATGGATTTGAACCATTTCTTCCCTAAGACAGCAGGTGCAGAGTCTTTCCCTAC
CAGCTGAATCCGAAATCCTTACTTCAGAGCAGCTACAAATTAGTACCTTAGAGGACTTGG
CAATTACAGGTTGGGGTGCTGGAATAGCCATGGTAGGGAAGCAGATTTCTTCTGTCTGGG
TCTACATCTTGTCTCCTCTACCTAGAGATTGTCTAAAACTCTCAACTCTGTATATGTTCC
AGGATGAGCTGAAAAAAGGTCGAGGCAGTACACCAACTTAGTGTTTTGATCTGATGTAGA
AAGAGATAGGATACTGCATACGTGCAGCACAGAGCCTCCACTAACCACACCCTTGATCAC
CCAAAGATCCCAGGTCAGTGATATGCTAGAAAACAAGCCCAGGAAAGGTATTTCCCTGTG
GTATGACCCTACCCCAGTGTCTATCATCTGCCTCTTTTTGTGATCACTTTCTGGCCTTGC
GGAGATGACAAACCTGTTCTGCCCTCAGTCTTCCATTAACTAAGTTCAACACCTAGCCCC
CTGACCTATCATACCTNCAGATCTGTGGCACCAGTAACTTGACTCCTATTGCTTTAACTC
TCAATCACGAANAGTNTTTACCATATTGCANAATACGTACCTTCCCCCCCACCCCACCAA
GAACAGGAGAAATCACTGTGGCGCCTTGGAATGCTAAAATGCACAGAGTCATGCCCAGAT
GTACATAAGCCTGTACGTAGCTCCTGCAATCATGAAACAGGCAGGTTGGTGTACAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_000276
Insert Size 5130 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000276.3, NP_000267.2
RefSeq Size 5165 bp
RefSeq ORF 2706 bp
Locus ID 4952
Cytogenetics Xq26.1
Domains RhoGAP, IPPc, Exo_endo_phos
Protein Families Druggable Genome
Protein Pathways Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary 'This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'
Transcript Variant: This variant (a) encodes isoform (a).

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