OGG1 (NM_016820) Human Untagged Clone

CAT#: SC109509

OGG1 (untagged)-Human 8-oxoguanine DNA glycosylase (OGG1), nuclear gene encoding mitochondrial protein, transcript variant 1c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGG1
• Synonyms: HMMH; HOGG1; MUTM; OGH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109509 sequence for NM_016820 edited (data generated by NextGen Sequencing)
  ATGCCTGCCCGCGCGCTTCTGCCCAGGCGCATGGGGCATCGTACTCTAGCCTCCACTCCT
  GCCCTGTGGGCCTCCATCCCGTGCCCTCGCTCTGAGCTGCGCCTGGACCTGGTTCTGCCT
  TCTGGACAATCTTTCCGGTGGAGGGAGCAAAGTCCTGCACACTGGAGTGGTGTACTAGCG
  GATCAAGTATGGACACTGACTCAGACTGAGGAGCAGCTCCACTGCACTGTGTACCGAGGA
  GACAAGAGCCAGGCTAGCAGGCCCACACCAGACGAGCTGGAGGCCGTGCGCAAGTACTTC
  CAGCTAGATGTTACCCTGGCTCAACTGTATCACCACTGGGGTTCCGTGGACTCCCACTTC
  CAAGAGGTGGCTCAGAAATTCCAAGGTGTGCGACTGCTGCGACAAGACCCCATCGAATGC
  CTTTTCTCTTTTATCTGTTCCTCCAACAACAACATCGCCCGCATCACTGGCATGGTGGAG
  CGGCTGTGCCAGGCTTTTGGACCTCGGCTCATCCAGCTTGATGATGTCACCTACCATGGC
  TTCCCCAGCCTGCAGGCCCTGGCTGGGCCAGAGGTGGAGGCTCATCTCAGGAAGCTGGGC
  CTGGGCTATCGTGCCCGTTACGTGAGTGCCAGTGCCCGAGCCATCCTGGAAGAACAGGGC
  GGGCTAGCCTGGCTGCAGCAGCTACGAGAGTCCTCATATGAGGAGGCCCACAAGGCCCTC
  TGCATCCTGCCTGGAGTGGGCACCAAGGTGGCTGACTGCATCTGCCTGATGGCCCTAGAC
  AAGCCCCAGGCTGTGCCCGTGGATGTCCATATGTGGCACATTGCCCAACGTGACTACAGC
  TGGCACCCTACCACGTCCCAGGCGAAGGGACCGAGCCCCCAGACCAACAAGGAACTGGGA
  AACTTTTTCCGGAGCCTGTGGGGACCTTATGCTGGCTGGGCCCAAGCNACTCCACCCTCC
  TACAGGTGCTGTTCAGTGCCGACCTGCGCCAATGCCGCCATGCTCAGGAGCCACCAGCAA
  AGCGCAGAAAGGGTTCCAAAGGGCCGGAAGGCTAGATGGGGCACCCTGGACAAAGAAATT
  CCCCAAGCACCTTCCCCTCCATTCCCCACTTCTCTCTCCCCATCCCCACCCAGTCTCATG
  TTGGGGAGGGGCCTCCCTGTGACTACCTCAAAGGCCAGGCACCCCCAAATCAAGCAGTCA
  GTTTGCACAACAAGATGGGGTGGGGGATATTGA
  
  Clone variation with respect to NM_016820.3
  948 g=>n;994 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016820 unedited
  TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGGCCCACAG
  GCTCTGGGGGCGGGAGAAGATAAGTCGCAAGGAGGGGGCGGGACCTACACCTCAGGAAAG
  CCGGAGAATTGGGGCACGAAGCGGGGCTTTGATGACCCGCAAAGGGCGAGGCATGCAGGA
  GGTGGAGGAATTAAGTGAAACAGGGAAGGTTGTTAAACAGCACCGTGTGGGCGAGGCCTT
  AAGGGTCGTGGTCCTTGTCTGGGCGGGGTCTTTGGGCGTCGACGAGGCCTGGTTCTGGGT
  AGGCGGGGCTACTACGGGGCGGTGCCTGCTGTGGAAATGCCTGCCCGCGCGCTTCTGCCC
  AGGCGCATGGGGCATCGTACTCTAGCCTCCACTCCTGCCCTGTGGGCCTCCATCCCGTGC
  CCTCGCTCTGAGCTGCGCCTGGACCTGGTTCTGCCTTCTGGACAATCTTTCCGGTGGAGG
  GAGCAAAGTCCTGCACACTGGAGTGGTGTACTAGCGGATCAAGTATGGACACTGACTCAG
  ACTGAGGAGCAGCTCCACTGCACTGTGTACCGAGGAGACAAGAGCCAGGCTAGCAGGCCC
  ACACCAGACGAGCTGGAGGCCGTGCGCAAGTACTTCCAGCTAGATGTTACCCTGGCTCAA
  CTGTATCACCACTGGGGTTCCGTGGACTCCCACTTCCAAGAGGTGGCTCAGAAATTCCAA
  GGTGTGCGACTGCTGCGACAAGACCCCATCGAATGCCTTTTCTCTTTTATCTGTTCCTTC
  AACAACACATCGCCCGCATCACTGGCATGGTGGAGCGGCTGTGCCCAGCTTTGGNACCCT
  CGCTCATNCAGCTTGATGATGTCACCTAACATGGCTTNNCCAGNCTGCNAGCCCTGGNCT
  GTGAGTAGGGCCAGAGTGGNAGCTCATCTCAGAAGCTGGCCTGGGCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016820 unedited
  ACATACAAATGTTTCTATTTTATTGTTCCTTCTTGTAATAAAGGGAAGATAAAACCATCC
  TTAGCGCTGTCTCCCTCAATATCCCCCACCCCATCTTGTTGTGCAAACTGACTGCTTGAT
  TTGGGGGTGCCTGGCCTTTGAGGTAGTCACAGGGAGGCCCCTCCCCAACATGAAACTGGG
  TGGGGATGGGGAGAGAGAAGTGGGGAATGGAGGGNAANGNNCTNGNNGNNNN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016820
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016820.2, NP_058213.1
• RefSeq Size: 2574 bp
• RefSeq ORF: 1233 bp
• Locus ID: 4968
• Cytogenetics: 3p25.3
• Domains: HHH, ENDO3c
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: 'This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]'
  Transcript Variant: Transcript variant 1c contains a 17 bp insertion before exon 7, as compared to the predominant transcript variant 1a.