P2Y2 (P2RY2) (NM_176072) Human Untagged Clone

CAT#: SC109532

P2RY2 (untagged)-Human purinergic receptor P2Y, G-protein coupled, 2 (P2RY2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2RY2
• Synonyms: HP2U; P2RU1; P2U; P2U1; P2UR; P2Y2; P2Y2R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109532 sequence for NM_176072 edited (data generated by NextGen Sequencing)
  ATGGCAGCAGACCTGGGCCCCTGGAATGACACCATCAATGGCACCTGGGATGGGGATGAG
  CTGGGCTACAGGTGCCGCTTCAACGAGGACTTCAAGTACGTGCTGCTGCCTGTGTCCTAC
  GGCGTGGTGTGCGTGCTTGGGCTGTGTCTGAACGCCGTGGCGCTCTACATCTTCTTGTGC
  CGCCTCAAGACCTGGAATGCGTCCACCACATATATGTTCCACCTGGCTGTGTCTGATGCA
  CTGTATGCGGCCTCCCTGCCGCTGCTGGTCTATTACTACGCCCGCGGCGACCACTGGCCC
  TTCAGCACGGTGCTCTGCAAGCTGGTGCGCTTCCTCTTCTACACCAACCTTTACTGCAGC
  ATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGTCTGGGCGTCTTACGACCTCTGCGC
  TCCCTGCGCTGGGGCCGGGCCCGCTACGCTCGCCGGGTGGCCGGGGCCGTGTGGGTGTTG
  GTGCTGGCCTGCCAGGCCCCCGTGCTCTACTTTGTCACCACCAGCGCGCGCGGGGGCCGC
  GTAACCTGCCACGACACCTCGGCACCCGAGCTCTTCAGCCGCTTCGTGGCCTACAGCTCA
  GTCATGCTGGGCCTGCTCTTCGCGGTGCCCTTTGCCGTCATCCTTGTCTGTTACGTGCTC
  ATGGCTCGGCGACTGCTAAAGCCAGCCTACGGGACCTCGGGCGGCCTGCCTAGGGCCAAG
  CGCAAGTCCGTGCGCACCATCGCCGTGGTGCTGGCTGTCTTCGCCCTCTGCTTCCTGCCA
  TTCCACGTCACCCGCACCCTCTACTACTCCTTCCGCTCGCTGGACCTCAGCTGCCACACC
  CTCAACGCCATCAACATGGCCTACAAGGTTACCCGGCCGCTGGCCAGTGCTAACAGTTGC
  CTTGACCCCGTGCTCTACTTCCTGGCTGGGCAGAGGCTCGTACGCTTTGCCCGAGATGCC
  AAGCCACCCACTGGCCCCAGCCCTGCCACCCCGGCTCGCCGCAGGCTGGGCCTGCGCAGA
  TCCGACAGAACTGACATGCAGAGGATAGAAGATGTGTTGGGCAGCAGTGAGGACTCTAGG
  CGGACAGAGTCCACGCCGGCTGGTAGCGAGAACACTAAGGACATTCGGCTGTAG
  
  Clone variation with respect to NM_176072.1
  137 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176072 unedited
  CGTCAAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGACGGCTTG
  AACGCGCCTGTGGGTCCGTGGTTGAACTTTGTCTCCACGGGAGCGTCGGTCTCGGAGCCC
  CTTGTGGCAGCAGCACTACCTGCCCAGAAAATGCTGGAGGCTGGGCGTGGCCCCAGGCCT
  GGGGACCTGTTTTTCCTGTTTCCCGCAGAGTTCCCTGCAGCCCGGTCCAGGTCCAGGCGT
  GTGCATTCATGAGTGAGGAACCCGTGCAGGCGCTGAGCATCCTGACCTGGAGAGCAGGGG
  CTGGTCAGGGCGATGGCAGCAGACCTGGGCCCCTGGAATGACACCATCAATGGCACCTGG
  GATGGGGATGAGCTGGGCTACAGGTGCCGCTTCAACGAGGACTTCAAGTACGTGCTGCTG
  CCTGTGTCCTACGGCGTGGTGTGCGTGCTTGGGCTGTGTCTGAACGCCGTGGCGCTCTAC
  ATCTTCTTGTGCCGCCTCAAGACCTGGAATGCGTCCACCACATATATGTTCCACCTGGCT
  GTGTCTGATGCACTGTATGCGGCCTCCCTGCCGCTGCTGGTCTATTACTACGCCCGCGGC
  GACCACTGGCCCTTCAGCACGGTGCTCTGCAAGCTGGTGCGCTTCCTCTTCTACACCAAC
  CTTTACTGCAGCATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGTCTGGGCGTCTTA
  CGACCTCTGCGCTCCCTGCGCTGGGGCCGGGCCCGCTACGCTCGCCGGGTGGCCCGNGCC
  GTGTGGGTGTTGGTGCTGGCCTGCCAGCCCCCGTGCTCTACTTTGTCACCACAGCGCGCG
  CGGGGGCCGCGTAACCTGCCACGACAACTCGGCACCCGAGCTTTTAGCCCCTTTCTGGCC
  TACAGCTCAGTCATGCCTGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_176072 unedited
  GGGTGTAGGGTTATTTAGATAATATAAAAAACGACCTCGTGGGTTAGTTTCNATTTGTGC
  CTAANATTTTTGCACCTGGNAAAGGGCAGTTCTACAAATAAGGGTTCCCAGTTAAATCGG
  AGCTCCAGTTAAATGGCTTAACATTTACCAGCACATGACTGGCCATCACCTAAACTGGGA
  AATCTCAAGGACTGGGACTGCACCTGACTTTTCTCTGTGCTCAGCACAGAGTCAGGCACA
  GAAGCCAACTGGCTTTACAGTGGGGGATGACCTGGGGCCTGGAGAGAGGATCCCTGATAT
  GAGACTACTCCTGAGACAGCTAGTGAGAAGGCAGGTGGCAGAAGGGAACGGATTGGAGAA
  AGGACCCTTGTGTGGCTGGTGAAAGCTTCCCTGCCATATCCTCATCCCCCGACCACACAA
  TAGGGCACAGCTCAGCAGGCAACACAACCTTTAGTATAAGTTACAGCCTCACACTGGCTC
  CTAGCCCATGGGAGCCTCCAGATGGGTCTATGATATTAGTCCCCAGATGTTCATCTCTTG
  GGCTGGGGGTACTCCTCAGAGCTAAGTCCACCGTGGCACCCAGTCCATTCCAGACCACTG
  TGCAAACTCAGCCCTCATTACTTACCAGGGTTTTCTGGCCAACCTGTGACTTGGCCCCCA
  CCCCAGGTAGCTCAGCTCCAGACTCTGGTATGTGATCTTGGTAAGCCACCTTCCTCTCTG
  GGCCTGTTTCTCCATTTGACTTGATTAGGCTGGACTCCAACCTANGTACCTTGGCAGTAC
  AGCCAGCTACTTGCATGGGAGTCAGGCCAGGGGTGTCATTGACCTTGAGCTCTTGCCTTT
  CTTGAACTTAATTCCCCCACTTATACACACAACGATGACTAGGGGTTATGGGGACAGTTG
  ACTCTGGACACAGAGTGATATTCCGAGCCCTGTCAATGACGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176072
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176072.1, NP_788086.1
• RefSeq Size: 2681 bp
• RefSeq ORF: 1134 bp
• Locus ID: 5029
• Cytogenetics: 11q13.4
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]'
  Transcript Variant: This variant (1) represents the longest transcript. Transcript variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.