P2Y2 (P2RY2) (NM_176072) Human Untagged Clone

CAT#: SC109532

P2RY2 (untagged)-Human purinergic receptor P2Y, G-protein coupled, 2 (P2RY2), transcript variant 1


  "NM_176072" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol P2RY2
Synonyms HP2U; P2RU1; P2U; P2U1; P2UR; P2Y2; P2Y2R
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109532 sequence for NM_176072 edited (data generated by NextGen Sequencing)
ATGGCAGCAGACCTGGGCCCCTGGAATGACACCATCAATGGCACCTGGGATGGGGATGAG
CTGGGCTACAGGTGCCGCTTCAACGAGGACTTCAAGTACGTGCTGCTGCCTGTGTCCTAC
GGCGTGGTGTGCGTGCTTGGGCTGTGTCTGAACGCCGTGGCGCTCTACATCTTCTTGTGC
CGCCTCAAGACCTGGAATGCGTCCACCACATATATGTTCCACCTGGCTGTGTCTGATGCA
CTGTATGCGGCCTCCCTGCCGCTGCTGGTCTATTACTACGCCCGCGGCGACCACTGGCCC
TTCAGCACGGTGCTCTGCAAGCTGGTGCGCTTCCTCTTCTACACCAACCTTTACTGCAGC
ATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGTCTGGGCGTCTTACGACCTCTGCGC
TCCCTGCGCTGGGGCCGGGCCCGCTACGCTCGCCGGGTGGCCGGGGCCGTGTGGGTGTTG
GTGCTGGCCTGCCAGGCCCCCGTGCTCTACTTTGTCACCACCAGCGCGCGCGGGGGCCGC
GTAACCTGCCACGACACCTCGGCACCCGAGCTCTTCAGCCGCTTCGTGGCCTACAGCTCA
GTCATGCTGGGCCTGCTCTTCGCGGTGCCCTTTGCCGTCATCCTTGTCTGTTACGTGCTC
ATGGCTCGGCGACTGCTAAAGCCAGCCTACGGGACCTCGGGCGGCCTGCCTAGGGCCAAG
CGCAAGTCCGTGCGCACCATCGCCGTGGTGCTGGCTGTCTTCGCCCTCTGCTTCCTGCCA
TTCCACGTCACCCGCACCCTCTACTACTCCTTCCGCTCGCTGGACCTCAGCTGCCACACC
CTCAACGCCATCAACATGGCCTACAAGGTTACCCGGCCGCTGGCCAGTGCTAACAGTTGC
CTTGACCCCGTGCTCTACTTCCTGGCTGGGCAGAGGCTCGTACGCTTTGCCCGAGATGCC
AAGCCACCCACTGGCCCCAGCCCTGCCACCCCGGCTCGCCGCAGGCTGGGCCTGCGCAGA
TCCGACAGAACTGACATGCAGAGGATAGAAGATGTGTTGGGCAGCAGTGAGGACTCTAGG
CGGACAGAGTCCACGCCGGCTGGTAGCGAGAACACTAAGGACATTCGGCTGTAG

Clone variation with respect to NM_176072.1
137 c=>t
>OriGene 5' read for NM_176072 unedited
CGTCAAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGACGGCTTG
AACGCGCCTGTGGGTCCGTGGTTGAACTTTGTCTCCACGGGAGCGTCGGTCTCGGAGCCC
CTTGTGGCAGCAGCACTACCTGCCCAGAAAATGCTGGAGGCTGGGCGTGGCCCCAGGCCT
GGGGACCTGTTTTTCCTGTTTCCCGCAGAGTTCCCTGCAGCCCGGTCCAGGTCCAGGCGT
GTGCATTCATGAGTGAGGAACCCGTGCAGGCGCTGAGCATCCTGACCTGGAGAGCAGGGG
CTGGTCAGGGCGATGGCAGCAGACCTGGGCCCCTGGAATGACACCATCAATGGCACCTGG
GATGGGGATGAGCTGGGCTACAGGTGCCGCTTCAACGAGGACTTCAAGTACGTGCTGCTG
CCTGTGTCCTACGGCGTGGTGTGCGTGCTTGGGCTGTGTCTGAACGCCGTGGCGCTCTAC
ATCTTCTTGTGCCGCCTCAAGACCTGGAATGCGTCCACCACATATATGTTCCACCTGGCT
GTGTCTGATGCACTGTATGCGGCCTCCCTGCCGCTGCTGGTCTATTACTACGCCCGCGGC
GACCACTGGCCCTTCAGCACGGTGCTCTGCAAGCTGGTGCGCTTCCTCTTCTACACCAAC
CTTTACTGCAGCATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGTCTGGGCGTCTTA
CGACCTCTGCGCTCCCTGCGCTGGGGCCGGGCCCGCTACGCTCGCCGGGTGGCCCGNGCC
GTGTGGGTGTTGGTGCTGGCCTGCCAGCCCCCGTGCTCTACTTTGTCACCACAGCGCGCG
CGGGGGCCGCGTAACCTGCCACGACAACTCGGCACCCGAGCTTTTAGCCCCTTTCTGGCC
TACAGCTCAGTCATGCCTGGCC
>OriGene 3' read for NM_176072 unedited
GGGTGTAGGGTTATTTAGATAATATAAAAAACGACCTCGTGGGTTAGTTTCNATTTGTGC
CTAANATTTTTGCACCTGGNAAAGGGCAGTTCTACAAATAAGGGTTCCCAGTTAAATCGG
AGCTCCAGTTAAATGGCTTAACATTTACCAGCACATGACTGGCCATCACCTAAACTGGGA
AATCTCAAGGACTGGGACTGCACCTGACTTTTCTCTGTGCTCAGCACAGAGTCAGGCACA
GAAGCCAACTGGCTTTACAGTGGGGGATGACCTGGGGCCTGGAGAGAGGATCCCTGATAT
GAGACTACTCCTGAGACAGCTAGTGAGAAGGCAGGTGGCAGAAGGGAACGGATTGGAGAA
AGGACCCTTGTGTGGCTGGTGAAAGCTTCCCTGCCATATCCTCATCCCCCGACCACACAA
TAGGGCACAGCTCAGCAGGCAACACAACCTTTAGTATAAGTTACAGCCTCACACTGGCTC
CTAGCCCATGGGAGCCTCCAGATGGGTCTATGATATTAGTCCCCAGATGTTCATCTCTTG
GGCTGGGGGTACTCCTCAGAGCTAAGTCCACCGTGGCACCCAGTCCATTCCAGACCACTG
TGCAAACTCAGCCCTCATTACTTACCAGGGTTTTCTGGCCAACCTGTGACTTGGCCCCCA
CCCCAGGTAGCTCAGCTCCAGACTCTGGTATGTGATCTTGGTAAGCCACCTTCCTCTCTG
GGCCTGTTTCTCCATTTGACTTGATTAGGCTGGACTCCAACCTANGTACCTTGGCAGTAC
AGCCAGCTACTTGCATGGGAGTCAGGCCAGGGGTGTCATTGACCTTGAGCTCTTGCCTTT
CTTGAACTTAATTCCCCCACTTATACACACAACGATGACTAGGGGTTATGGGGACAGTTG
ACTCTGGACACAGAGTGATATTCCGAGCCCTGTCAATGACGAG
Restriction Sites NotI-NotI     
ACCN NM_176072
Insert Size 3000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176072.1, NP_788086.1
RefSeq Size 2681 bp
RefSeq ORF 1134 bp
Locus ID 5029
Cytogenetics 11q13.4
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary 'The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]'
Transcript Variant: This variant (1) represents the longest transcript. Transcript variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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