PTBP1 (NM_031991) Human Untagged Clone

CAT#: SC109649

PTBP1 (untagged)-Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTBP1
• Synonyms: HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031991, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAAAGCGGGGATCTGACGAGCTTTTCTCTACTTGTG
  TCACTAACGGACCGTTTATCATGAGCAGCAACTCGGCTTCTGCAGCAAACGGAAATGACAGCAAGAAGTT
  CAAAGGTGACAGCCGAAGTGCAGGCGTCCCCTCTAGAGTGATCCACATCCGGAAGCTCCCCATCGACGTC
  ACGGAGGGGGAAGTCATCTCCCTGGGGCTGCCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGGGGA
  AAAACCAGGCCTTCATCGAGATGAACACGGAGGAGGCTGCCAACACCATGGTGAACTACTACACCTCGGT
  GACCCCTGTGCTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTGAAGACCGACAGC
  TCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGGCGGTGAACTCGGTCCAGTCGGGGAACCTGGCCT
  TGGCTGCCTCGGCGGCGGCCGTGGACGCAGGGATGGCGATGGCCGGGCAGAGCCCCGTGCTCAGGATCAT
  CGTGGAGAACCTCTTCTACCCTGTGACCCTGGATGTGCTGCACCAGATTTTCTCCAAGTTCGGCACAGTG
  TTGAAGATCATCACCTTCACCAAGAACAACCAGTTCCAGGCCCTGCTGCAGTATGCGGACCCCGTGAGCG
  CCCAGCACGCCAAGCTGTCGCTGGACGGGCAGAACATCTACAACGCCTGCTGCACGCTGCGCATCGACTT
  TTCCAAGCTCACCAGCCTCAACGTCAAGTACAACAATGACAAGAGCCGTGACTACACACGCCCAGACCTG
  CCTTCCGGGGACAGCCAGCCCTCGCTGGACCAGACCATGGCCGCGGCCTTCGGCCTTTCCGTTCCGAACG
  TCCACGGCGCCCTGGCCCCCCTGGCCATCCCCTCGGCGGCGGCGGCAGCTGCGGCGGCAGGTCGGATCGC
  CATCCCGGGCCTGGCGGGGGCAGGAAATTCTGTATTGCTGGTCAGCAACCTCAACCCAGAGAGAGTCACA
  CCCCAAAGCCTCTTTATTCTTTTCGGCGTCTACGGTGACGTGCAGCGCGTGAAGATCCTGTTCAATAAGA
  AGGAGAACGCCCTAGTGCAGATGGCGGACGGCAACCAGGCCCAGCTGGCCATGAGCCACCTGAACGGGCA
  CAAGCTGCACGGGAAGCCCATCCGCATCACGCTCTCGAAGCACCAGAACGTGCAGCTGCCCCGCGAGGGC
  CAGGAGGACCAGGGCCTGACCAAGGACTACGGCAACTCACCCCTGCACCGCTTCAAGAAGCCGGGCTCCA
  AGAACTTCCAGAACATATTCCCGCCCTCGGCCACGCTGCACCTCTCCAACATCCCGCCCTCAGTCTCCGA
  GGAGGATCTCAAGGTCCTGTTTTCCAGCAATGGGGGCGTCGTCAAAGGATTCAAGTTCTTCCAGAAGGAC
  CGCAAGATGGCACTGATCCAGATGGGCTCCGTGGAGGAGGCGGTCCAGGCCCTCATTGACCTGCACAACC
  ACGACCTCGGGGAGAACCACCACCTGCGGGTCTCCTTCTCCAAGTCCACCATCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031991 unedited
  NTTGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCCACTCC
  GTCCCCCGCGGGTCTGCTCTGTGTGCCATGGACGGCATTGTCCCAGATATAGCCGTTGGT
  ACAAAGCGGGGATCTGACGAGCTTTTCTCTACTTGTGTCACTAACGGACCGTTTATCATG
  AGCAGCAACTCGGCTTCTGCAGCAAACGGAAATGACAGCAAGAAGTTCAAAGGTGACAGC
  CGAAGTGCAGGCGTCCCCTCTAGAGTGATCCACATCCGGAAGCTCCCCATCGACGTCACG
  GAGGGGGAGGTCATCTCCCTGGGGCTGCCCTTTGGGAAGGTCACCAACCTCCTGATGCTG
  AAGGGGAAAAACCAGGCCTTCATCGAGATGAACACGGAGGAGGCTGCCAACACCATGGTG
  AACTACTACACCTCGGTGACCCCTGTGCTGCGCGGCCAGCCCATCTACATCCAGTTCTCC
  AACCACAAGGAGCTGAAGACCGACAGCTCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTG
  CAGGCGGTGAACTCGGTCCAGTCGGGGAACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTG
  GACGCAGGGATGGCGATGGCCGGGCAGAGCCCCGTGCTCAGGATCATCGTGGAGAACCTC
  TTCTACCCTGTGACCCTGGATGTGCTGCACCAGATTTTCTCCAAGTTCGGCACAGTGTTN
  GAAGATCATCACCTTCACCAAGAACAACCAGTTCCAGGCCCTGCTGCAGTATGCGGACCC
  CGTGAGCGCCCAGCACGCCAAGCTGTCGCTGGACGGGCAGAACATCTACAACGCCTGCTG
  CACGCTGCGCATCGACTTTTNCAGCTCACAGCCTCNACGTCAGTACAACATGACAAGAGC
  CGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031991 unedited
  CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGGATACANAAGATTTATTC
  GAAGTCCAGGTACAGACTGGCCAACCTGCCTCTACAGCGTCCACAGCGAACACAGGGCTA
  GACAAGGGAGGAGTTTCTCAAACGGTTTTAATCGGTTCTCTCCGCGTCACAAGCCATCGG
  GTAAGGCAACGGAATGTGCGTGGGGTCCCCTGTGGCTCCGCGGTCACAATACTGAGCCTG
  GAATTGCTGTTAGCAAAATATACATTTGTGTCACCATAAAAAACCGCGCCGCCGCCCCTC
  GGGTCTCACAACAGGTATAAAAAATTATAAATATTTACACCCTTGTTACACGCTTTTACG
  GAAAGGGGATCCTAGGAGAGCCCCCGGGACAGGACGCGGGGGCGGTAGAAAGAGCACAGA
  GAAGACAGGAGGAGCGCCCGCCTTCCGGGTCCCAGCATCAGAGGCAGGCAAAACACAGAA
  ATGAGACTTTGGTCCAAAATTTGAAGGAAGAAAAAACCCTGAAGTTTGAGAGCAAGTTTT
  CTAGGCGACTGCAGGTGAAGGCGTGGTGTAAGGCAACCCGAGGCGATGCCAGAGGCGGCG
  ACACGCCCGCCCTTTCCCTGAAGCTCAGGNGCTCTAAGATAGGACCCGGGCTCCCTGTGC
  CTGACCCACNNTGGCGNCCCGNCTGCTCTCTGGNAACTGGTCCTGCCTGTGACTTATTAC
  AAGGAATAAATAAAAGTAACAGCCTACANGGTTATACCCCAGGAAGTTTAGATTACCCTC
  ATACCATTGNATCATCCTGCTATAAAAAAGTACTCTAAACTATTTCTTTTGCTATTAATG
  AAAAAGATTAAATATTTGGTCACCTGGAATATTGCTATGCCAGACCTTCGCAACTGCAAG
  TATTTAATCCGAGATATGTCNACCCCTCCCAACTGACCCCGGTGAGCCCCCTGGGGCAAA
  AGGAAGCCCTTGGCCGGTAAAGGAAATCCGGACTGATATAGCTTGGTTGGGGTGCCACCC
  GGCCCCGGCCANTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031991
• Insert Size: 3260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031991.2, NP_114368.1
• RefSeq Size: 3241 bp
• RefSeq ORF: 1596 bp
• Locus ID: 5725
• Cytogenetics: 19p13.3
• Domains: RRM
• Protein Families: Druggable Genome
• Gene Summary: 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks 78 nt in the coding region compared to variant 1. Thus isoform c is 26 aa shorter than isoform a but it maintains the same reading frame.