PTP epsilon (PTPRE) (NM_130435) Human Untagged Clone

CAT#: SC109677

PTPRE (untagged)-Human protein tyrosine phosphatase, receptor type, E (PTPRE), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRE
• Synonyms: HPTPE; PTPE; R-PTP-EPSILON
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109677 sequence for NM_130435 edited (data generated by NextGen Sequencing)
  ATGAGCAACAGGAGTAGCTTTTCCCGGCTCACCTGGTTCAGGAAGCAGAGGAAAGCTGTG
  GTCAGCACCAGCGACAAGAAGATGCCCAACGGAATCTTGGAGGAGCAAGAGCAGCAAAGG
  GTGATGCTGCTCAGCAGGTCACCCTCAGGGCCCAAGAAGTATTTTCCCATCCCCGTGGAG
  CACCTGGAGGAGGAGATCCGTATCAGATCCGCCGACGACTGCAAGCAGTTTCGGGAGGAG
  TTCAACTCATTGCCATCTGGACACATACAAGGAACTTTTGAACTGGCAAATAAAGAAGAA
  AACAGAGAAAAAAACAGATATCCCAACATCCTTCCCAATGACCATTCTAGGGTGATTCTG
  AGCCAACTGGATGGAATTCCCTGTTCAGACTACATCAATGCTTCCTACATAGATGGTTAC
  AAAGAGAAGAATAAATTCATAGCAGCTCAAGGTCCCAAACAGGAAACGGTTAACGACTTC
  TGGAGAATGGTCTGGGAGCAAAAGTCTGCGACCATCGTCATGTTAACAAACTTGAAAGAA
  AGGAAAGAGGAAAAGTGCCATCAGTACTGGCCCGACCAAGGCTGCTGGACCTATGGAAAC
  ATCCGGGTGTGCGTGGAGGACTGCGTGGTTTTGGTCGACTACACCATCCGGAAGTTCTGC
  ATACAGCCACAGCTCCCCGACGGCTGCAAAGCCCCCAGGCTGGTCTCACAGCTGCACTTC
  ACCAGCTGGCCCGACTTCGGAGTGCCTTTTACCCCCATTGGGATGCTGAAGTTCCTCAAG
  AAAGTAAAGACGCTCAACCCCGTGCACGCTGGGCCCATCGTGGTCCACTGTAGCGCGGGC
  GTGGGCCGGACGGGCACCTTCATTGTGATCGATGCCATGATGGCCATGATGCACGCGGAG
  CAGAAGGTGGATGTGTTTGAATTTGTGTCTCGAATCCGTAATCAGCGCCCTCAGATGGTT
  CAAACGGATATGCAGTACACGTTCATCTACCAAGCCTTACTCGAGTACTACCTCTACGGG
  GACACAGAGCTGGACGTGTCCTCCCTGGAGAAGCACCTGCAGACCATGCACGGCACCACC
  ACCCACTTCGACAAGATCGGGCTGGAGGAGGAGTTCAGGAAATTGACAAATGTCCGGATC
  ATGAAGGAGAACATGAGGACGGGCAACTTGCCGGCAAACATGAAGAAGGCCAGGGTCATC
  CAGATCATCCCGTATGACTTCAACCGAGTGATCCTTTCCATGAAAAGGGGTCAAGAATAC
  ACAGACTACATCAACGCATCCTTCATAGACGGCTACCGACAGAAGGACTATTTCATCGCC
  ACCCAGGGGCCACTGGCACACACGGTTGAGGACTTCTGGAGGATGATCTGGGAATGGAAA
  TCCCACACTATCGTGATGCTGACGGAGGTGCAGGAGAGAGAGCAGGATAAATGCTACCAG
  TATTGGCCAACCGAGGGCTCAGTTACTCATGGAGAAATAACGATTGAGATAAAGAATGAT
  ACCCTTTCAGAAGCCATCAGTATACGAGACTTTCTGGTCACTCTCAATCAGCCCCAGGCC
  CGCCAGGAGGAGCAGGTCCGAGTAGTGCGCCAGTTTCACTTCCACGGCTGGCCTGAGATC
  GGGATTCCCGCCGAGGGCAAAGGCATGATTGACCTCATCGCAGCCGTGCAGAAGCAGCAG
  CAGCAGACAGGCAACCACCCCATCACCGTGCACTGCAGTGCCGGAGCTGGGCGAACAGGT
  ACATTCATAGCCCTCAGCAACATTTTGGAGCGAGTAAAAGCCGAGGGACTTTTAGATGTA
  TTTCAAGCTGTGAAGAGTTTACGACTTCAGAGACCACATATGGTGCAAACCCTGGAACAG
  TATGAATTCTGCTACAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTAAT
  TTCAAATGA
  
  Clone variation with respect to NM_130435.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130435 unedited
  CGTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACCAGGTGGCTCAGC
  CATGAGCAACAGGAGTAGCTTTTCCCGGCTCACCTGGTTCAGGAAGCAGAGGAAAGCTGT
  GGTCAGCACCAGCGACAAGAAGATGCCCAACGGAATCTTGGAGGAGCAAGAGCAGCAAAG
  GGTGATGCTGCTCAGCAGGTCACCCTCAGGGCCCAAGAAGTATTTTCCCATCCCCGTGGA
  GCACCTGGAGGAGGAGATCCGTATCAGATCCGCCGACGACTGCAAGCAGTTTCGGGAGGA
  GTTCAACTCATTGCCATCTGGACACATACAAGGAACTTTTGAACTGGCAAATAAAGAAGA
  AAACAGAGAAAAAAACAGATATCCCAACATCCTTCCCAATGACCATTCTAGGGTGATTCT
  GAGCCAACTGGATGGAATTCCCTGTTCAGACTACATCAATGCTTCCTACATAGATGGTTA
  CAAAGAGAAGAATAAATTCATAGCAGCTCAAGGTCCCAAACAGGAAACGGTTAACGACTT
  CTGGAGAATGGTCTGGGAGCAAAAGTCTGCGACCATCGTCATGTTAACAAACTTGAAAGA
  AAGGAAAGAGGAAAAGTGCCATCAGTACTGGCCCGACCAAGGCTGCTGGACCTATGGAAA
  CATCCGGGTGTGCGTGGAGGACTGCGTGGTTNTGGTCGACTACACCATCCGGAAGTTCTG
  CATACAGCCACAGCTCCCCGACGGCTGCANAGCCCCCAGGCTGGTCTCACAGCTGCACTT
  CACCAGCTGGCCCGACTTCGNAGTGCCTTTTACCCCCATTGGGATGCTGAAGTTCCCTCA
  GAAAGTAAGACGCTCAACCCGTGCACGCTGGGCCCATCGTGGTCCACTGNTACGCGGGCG
  TGGGCCGGNACGGCACCTCCATGTGATCGATCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130435
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130435.2, NP_569119.1
• RefSeq Size: 5002 bp
• RefSeq ORF: 1929 bp
• Locus ID: 5791
• Cytogenetics: 10q26.2
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Several alternatively spliced transcript variants of this gene have been reported, at least two of which encode a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokine-induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (2) contains an alternate exon in place of the first 5 exons compared to variant 3. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 3. The encoded protein is a cytoplasmic isoform of this PTP.