RAD17 (NM_133339) Human Untagged Clone

CAT#: SC109696

RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD17
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133339, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCAAAAACTTTTCTTAGACCAAAGGTATCTTCCACAAAGGTAACAGACTGGGTTGACCCATCATTTG
  ATGATTTTCTAGAGTGTAGTGGCGTCTCTACTATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCA
  TAGAAGAAAAAATGGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCT
  TCCTTAGAACAGATTTATGGTTTAGAAAATTCAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATA
  AATATAAACCAGAAACTCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTT
  AAAAGCTCAAGTTTTAGAAAGGCAACCAAAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGA
  TGTGGAAAGACAACGACCTTAAAAATACTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATC
  CAGTTTTACCAGACTTCCAAAAAGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTT
  TCCCTATCAGTCTCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAA
  ATGCTTGGAGATGATCTGAGAACTGATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATC
  GGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTAT
  AATCTCGGACAGTCTCAGTGGAGATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGT
  TCTATCTCAAATATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGA
  CTATAGAAGCTAACAAGAATGGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCA
  GGGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAAC
  TTACGGCCAAGGAAAAAAGGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAA
  AACCTGATAGGGTTTTTGAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTT
  CAGAGCTTTGGGGAAAATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCC
  TCTCATTTATCAGAATATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACA
  TGCCTGGAGACTTATTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGATGATAT
  TGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTC
  AGGGAATATAGCACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATT
  GCCAAGGAGGAGGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCG
  GGAAAATTGCCTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAG
  CTATTGCCATACCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATA
  TTGGAAGGCTCCCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGG
  AATGATAGACCCTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGT
  GAACCCACTCAAGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGC
  CTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGACTACGA
  GAGTGATGGGACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133339 unedited
  NGCGCTTGTATTGTAAACGACTTACTATAGGCGGCCGCGAATTCGCACCAGGCGTTGAGC
  CCGGGTAGGGCCAGGTGGCTGCCCTTTCACCTAGGGTAGTCCCTGGTCGCCTCCGCTCTT
  CGCCCAAAAGGGATGCAGCTCCGGGAAACAAGTGAATTCATGGTATTTTACTTTTTTGGG
  AAATACTGGAAATGAAGACCTGCAACTGTTATTTGAAATAAGGAAAACTTTAATTTTCAG
  TATAAAAATTGCTCAAATAGAATTGCCTGATTTTAATGACAAAAGGTGAATTATAGTTTA
  ATGTACTGCAAGTCCTAAACTACGGATGGGAACTATTACAGTTTATAATGTCAAAAACTT
  TTCTTAGACCAAAGGTATCTTCCACAAAGTATATGGGAGTCCACATTTATGTAAGAAATG
  AAACTATAAAATGTATAAATAATTTGCAAATCAGAATTGCTGTCGAAAGTTTTACTATAA
  TGAAAGATATTTTCATACTCTCAAAAATATAGAGGAAAGGGGCCAAGATTATAGTACCAG
  TCACAATCTTTTGATGAGGACGAAATGAATCAGGTAACAGACTGGGTTGACCCATCATTT
  GATGATTTTCTAGAGTGTAGTGGCGTCTCTACTATTACTGCCACATCATTAGGTGTGAAT
  AACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCG
  AGAAAAAGAGGAATCTATCTTCCTTAAAACAGATTTATGGTTTTAGAAAATCAAAAGAAT
  ATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCCGCATGAACTTG
  CTGTGCATAAAAAGAAATTGGAGAAGTCGAAACCTGGTTAAAGCTCAAGTTTAGAAAGCA
  ACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133339 unedited
  CNAGTAAAAACCTTTTTTTTTTAAATTCAACATTTTAATTGTGATATAACTTATGTAAAT
  ACTTTCCAAAAGTATCTAAGATATTCCACTGTACAACACATCAAAACTATCTGAGAAGCG
  GGAGACAATAAAGTGTAAATAAGGCACACAGTTTACACTCGTAATTGCATCCTAAACTGT
  TGAATAGTTGATATTTACAACCCCCCAATAACGTTTTTGCATCCTGAATTAGTCCTTCAC
  ATCTTGATATTTGGCATAATGTTCAAACACACTTTATACACACTGACCGCTCCTTAAACC
  ACTCACAGATTTAATAAATACCGAAAAAATTAAGAGATTTGAAGGCTTGATCTTCTATTT
  ACTTCAAGATGACAGAAGAGTAGGTTTCTTGTGATGAAATACTACAAGAATGAAGAATTA
  ACAAACTGTTGTGTAATTCATCAGAAAAGCATATTAACTCTGCTGAAGTACCACTGAATG
  AAACAAAAATGAAGCTGTGAAGTAGCAATCTGATTAGCAGGCTGGCTTCTATGTCCCATC
  ACTCTCGTAGTCTTCTATTATTATGTTTTCTTCCATGTCTCCTTGGGCTGAAAAGGGCTG
  GGGCTGGCTAGCAGGCAGTTCACTGGCACTATTCTGACTCAAAGGAAGAGACCAGGTTTC
  CGGCACAGTGGCTTGAGTGGGTTCACCCAGAGATTCCTCTGCAGAATGTCCTCCATTAAG
  CTGGGCTTCATCTCCGCTGTCAGGGTCTATCATTCCATGTTCCCTGTCAGTCAGGGCTTC
  CATTTTCAAATCTTCCAAAGTGTCGCTTCAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133339
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133339.1, NP_579917.1
• RefSeq Size: 2633 bp
• RefSeq ORF: 2046 bp
• Locus ID: 5884
• Cytogenetics: 5q13.2
• Domains: Rad17
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an alternate upstream translation start codon, when compared to variant 1. The resulting protein (isoform 2) is longer and has a distinct N-terminus, as compared to isoform 1.