RAD17 (NM_133340) Human Untagged Clone

CAT#: SC109697

RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD17
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133340, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTTTTCAAAGAGTTTC
  TACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGAAGATAAT
  TCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTAT
  GTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATAATCAAAGGT
  TATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGGCACCAAC
  AATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAATTACTGTC
  CCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCC
  TCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAAGGAATGTCTTTAAAATCAGA
  TGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTTGAAAATCAAGAGGTCCAAGCT
  ATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAGAGCAT
  CTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACATTACTTGT
  TGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCACCAAAAC
  TACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGATATCC
  TCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACATCTATAGCTACGAGAGGTGTGAT
  GCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTTGCACAAA
  CCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCCTGACT
  TCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCAATGAG
  AAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAAGATTG
  AAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCCCAGCTTA
  ATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCTGGTCTCT
  TCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGAC
  ATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133340 unedited
  GGGGTCAAAAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGNAAAAAA
  GGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAATTCAAAAGATATCTGTCTG
  AAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTGTGCATA
  AAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAA
  AACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCT
  TAAAAATACTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTAC
  CAGACTTCCAAAAAGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGT
  TTCCCTATCAGTCTCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATA
  ACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGAAGATAATTCTGGTTGAAG
  ATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTATG
  TGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATA
  ATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTT
  TCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAG
  CTAACAAGAATGGANGAAAAATTACTGTCCCTGACAAACTTCTCTAAGTTGCTCTGTCAG
  GGATGTTCTGGGTGATATCAGAGTGCAATAACAGCCTNCAGTTNTCTTCTTCAAAAGAGA
  NNAACACTACNGNCANGNAAAAAGGNATGTCTTAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_133340
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133340.1, NP_579918.1
• RefSeq Size: 2666 bp
• RefSeq ORF: 1518 bp
• Locus ID: 5884
• Cytogenetics: 5q13.2
• Domains: Rad17
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (3) lacks two consecutive exons in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus, compared to isoform 1.