p107 (RBL1) (NM_002895) Human Untagged Clone

CAT#: SC109714

RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1


  "NM_002895" in other vectors (6)

Reconstitution Protocol

USD 2,160.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RBL1
Synonyms CP107; p107; PRB1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109714 sequence for NM_002895 edited (data generated by NextGen Sequencing)
ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGAGTTTGAAAGATATCAACAACATG
ATAAGGCAAGGTGAGCAGAGAACCAAGAAGCGAGTAATAGCCATCGATAGTGATGCAGAA
TCCCCTGCCAAACGCGTCTGTCAAGAAAATGATGACGTTTTACTGAAACGACTACAGGAT
GTTGTCAGTGAAAGAGCAAATCATTAA

Clone variation with respect to NM_002895.2
>OriGene 5' read for NM_002895 unedited
CGGCCGCGAATTCGGCACGAGAAGTCGGGGGCCGTGGCGCGCAGCCCGCGGGGCCTGAAG
GGATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGG
CGCTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGG
ACGACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGG
CATGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCA
TGGAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATAC
AATTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTG
AACGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATG
AGCCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAA
GCCGGAAGCAGAGGAGGATTCCTTGCAGGGTTAAGGATCTGTTTAATTTCTGGTGGACAC
TTTTTGGTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATC
ATTTACTTCTATGCTGGCTGGATCTGAATTTTGCGCATGCGATTATGTGCCCAATAGACA
AGACTTGCTAATTCATCATTAN
>OriGene 3' read for NM_002895 unedited
CGCCCAATACGGTGTGCACACGCCCAATTTCGAGGATCNATTTTTCCCTTTTTTTTTTTT
TCCAATCCTTTTTTATAGCCTTCTAGTACTTGAACCAATCCTGAAAACTGAAAAAACTGT
CCAGAATTCTTTTTTTCCTTTTCTGAAAAAGGTCTTGCCCTCTCACCTAGGATGGAGTGT
AGTGGTGCAATCACAGCTCACTGCAGCCTCAAAATCTCCCAGTCTCAAGTGATCCTCTCA
TGTCAGTCTCCTGAGTAGCTGGGACTACAGGCACATGCCATCACACCCAACTAATTTTTT
TATTTTTTGTAAAGATGAGGGCTATGTTGCCCAGGTTAGTCTTGAACTCCTGGACTGAAG
CAATCCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTATAGGCATGAGCCACAGGACCTG
GCCCATGGTTTCTTATACAGTCAATGTACAAATGCTCATTTATATTTTGCACAATAATGA
AGATAAAAATAACAATTATAGCAACATACAAATATGTACAAAGATTCCAGACAGACTTTG
TTTTTTGGCTTATAACAATGTGTAGATACTACACAAAAAATGAGGATGTAATTTTCATTT
ACAAGCAAAATGTGACCCAAATCCCTTTTCTTCTTAAAATTGAAAAAAGAAATTCTTGGA
AAAACTAATTAGTGACGGCCAAATCTTAAACTATTTTAAATTACCCATGGTTAAACATAG
GTGAGTTAAACATGTGCCCTTCCAAATTAAGTTTGCCGTTAAAACATAACATTTGATAAA
ACTTCCAAAATAATTATGAGTGGCTTATTCAGTCCTTTGAATCCAGACCCCACTAAAACA
ATTAACTTTAAAAGTGATATTTTTAAAACCACCC
Restriction Sites NotI-NotI     
ACCN NM_002895
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002895.2, NP_002886.2
RefSeq Size 4270 bp
RefSeq ORF 3207 bp
Locus ID 5933
Cytogenetics 20q11.23
Domains RB_B, RB_A
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Cell cycle, TGF-beta signaling pathway
Gene Summary 'The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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