p107 (RBL1) (NM_002895) Human Untagged Clone

CAT#: SC109714

RBL1 (untagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBL1
• Synonyms: CP107; p107; PRB1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109714 sequence for NM_002895 edited (data generated by NextGen Sequencing)
  ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
  CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
  GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
  TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
  GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
  TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
  CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
  CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
  CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
  TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
  TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
  GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
  GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
  GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
  AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
  GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
  TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
  CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
  AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
  GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
  GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
  CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
  CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
  CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
  CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
  TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
  TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
  ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
  CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
  TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
  AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
  AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
  CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
  TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
  TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
  ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
  CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
  ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
  GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
  AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
  GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
  TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
  GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
  TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
  AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
  GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
  GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
  AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
  CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
  CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
  GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGAGTTTGAAAGATATCAACAACATG
  ATAAGGCAAGGTGAGCAGAGAACCAAGAAGCGAGTAATAGCCATCGATAGTGATGCAGAA
  TCCCCTGCCAAACGCGTCTGTCAAGAAAATGATGACGTTTTACTGAAACGACTACAGGAT
  GTTGTCAGTGAAAGAGCAAATCATTAA
  
  Clone variation with respect to NM_002895.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002895 unedited
  CGGCCGCGAATTCGGCACGAGAAGTCGGGGGCCGTGGCGCGCAGCCCGCGGGGCCTGAAG
  GGATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGG
  CGCTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGG
  ACGACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGG
  CATGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCA
  TGGAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATAC
  AATTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTG
  AACGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATG
  AGCCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAA
  GCCGGAAGCAGAGGAGGATTCCTTGCAGGGTTAAGGATCTGTTTAATTTCTGGTGGACAC
  TTTTTGGTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATC
  ATTTACTTCTATGCTGGCTGGATCTGAATTTTGCGCATGCGATTATGTGCCCAATAGACA
  AGACTTGCTAATTCATCATTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002895 unedited
  CGCCCAATACGGTGTGCACACGCCCAATTTCGAGGATCNATTTTTCCCTTTTTTTTTTTT
  TCCAATCCTTTTTTATAGCCTTCTAGTACTTGAACCAATCCTGAAAACTGAAAAAACTGT
  CCAGAATTCTTTTTTTCCTTTTCTGAAAAAGGTCTTGCCCTCTCACCTAGGATGGAGTGT
  AGTGGTGCAATCACAGCTCACTGCAGCCTCAAAATCTCCCAGTCTCAAGTGATCCTCTCA
  TGTCAGTCTCCTGAGTAGCTGGGACTACAGGCACATGCCATCACACCCAACTAATTTTTT
  TATTTTTTGTAAAGATGAGGGCTATGTTGCCCAGGTTAGTCTTGAACTCCTGGACTGAAG
  CAATCCTCCTGCCTCAGCCTCCCAAAGTGTTGGGATTATAGGCATGAGCCACAGGACCTG
  GCCCATGGTTTCTTATACAGTCAATGTACAAATGCTCATTTATATTTTGCACAATAATGA
  AGATAAAAATAACAATTATAGCAACATACAAATATGTACAAAGATTCCAGACAGACTTTG
  TTTTTTGGCTTATAACAATGTGTAGATACTACACAAAAAATGAGGATGTAATTTTCATTT
  ACAAGCAAAATGTGACCCAAATCCCTTTTCTTCTTAAAATTGAAAAAAGAAATTCTTGGA
  AAAACTAATTAGTGACGGCCAAATCTTAAACTATTTTAAATTACCCATGGTTAAACATAG
  GTGAGTTAAACATGTGCCCTTCCAAATTAAGTTTGCCGTTAAAACATAACATTTGATAAA
  ACTTCCAAAATAATTATGAGTGGCTTATTCAGTCCTTTGAATCCAGACCCCACTAAAACA
  ATTAACTTTAAAAGTGATATTTTTAAAACCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002895
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002895.2, NP_002886.2
• RefSeq Size: 4270 bp
• RefSeq ORF: 3207 bp
• Locus ID: 5933
• Cytogenetics: 20q11.23
• Domains: RB_B, RB_A
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.