ROBO1 (NM_133631) Human Untagged Clone

CAT#: SC109740

ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2


  "NM_133631" in other vectors (6)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ROBO1
Synonyms DUTT1; SAX3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_133631 edited
GAATTCGGCACCAGGCAAGACCGTGGAGGCAGGAAACGGCACTACTGCGCTTCTGCCTCG
GCTCTTTGTTGTTCGCTTTGGATGGTTCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTG
GGGCCGGAGAAGACAAACCTTGGAATTCTTCCTCTGCAAAAGTCTCTGAGATACTGACAA
GCGTCCGGAAAGGTCGACGAGTAATTGCCCTGAAAACTCTTGGCTAATTGACCCACGTTG
CTTATATTAAGCCTTTGTGTGTGGTGTGTGGCTTCATACATTTGGGGACCCTATTTCCAC
TCCCTCCTCTTGGCATGAGACTGTATACAGGATCCACCCGAGGACAATGATTGCGGAGCC
CGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGA
AGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACC
TGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGG
GGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAG
TGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGATGAAGGAGT
CTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGGA
AGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGG
AGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATG
GAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAA
GCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAA
TATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATT
TGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATTTAAATGTGA
GGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCAA
ATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGA
CATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTAC
TCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGT
TGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGC
TATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTC
ATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGATC
TGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGC
ATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCC
TGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAG
TCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCG
AATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGG
TCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGA
AGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATCCC
TAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTG
GCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCA
TGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCAT
TAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGG
AATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAG
TCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCA
CAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAGTC
TCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATC
AGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCT
CAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGC
AGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGG
TGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACC
TCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGA
AACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCCTT
TCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGG
GGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGA
GGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGC
AGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACA
CCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTT
TACCTTCACACCAACAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAG
GCCTGGACTTCTCAACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCC
TAATACTGGCAACAACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAA
CAGCGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAA
CCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGT
GGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGG
GCGTTTTGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTC
AAACCTCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACC
ACTGGGACAGCAGAAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAA
GCTGAACAAAGATTATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATC
ATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGG
GAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAA
CTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGA
GTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGC
AAGGATGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCC
CCCTGTTCGGGGAGCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGC
CACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGA
GACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACC
ACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGA
TATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGAT
GCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGA
CCTGGAGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGA
CAACATTTCCAGCGGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGC
TGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACG
GCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCAC
AAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGC
CAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCC
ACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCT
GGAAGTACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATC
ATCAGACAGAAAAGGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGT
TGACATGCGAACAAATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAA
AGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCA
AGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCC
CAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAA
TGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGA
TAATAATGAAGAATTAGAGGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCT
AATGTGAAAATCATCACTCAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAAT
GTTCAGTGCAATCAGAGTGTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTT
TAAAAACTTTATTGGGTTTTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTC
CTATTCCCCTCGCTGTTGGAGCAAACCATTATACCTTACTTCCAGCAAGCAAAGTGCTTT
GACTTCTTGCTTCAGTCATCAGCCAGCAAGAGGGAACAAAACTGTTCTTTTGCATTTTGC
CGCTGAGATATGGCATTGCACTGCTTATATGCCAAGCTAATTTATAGCAAGATATTGATC
AAATATAGAAAGTTGATATTCAACCTCACAAGGGCTCTCAAAGTATAATCTTTCTATAGC
CAACTGCTAATGCAAATTAAAACATATTTCATTTTAACATGATTTCAAAATCAGTTTTTC
ATACTACCCTTTGCTGGAAGAAACTAAAAATATAGCAAATGCAGAACCACAAACAATTCG
AATGGGGTAGAAACATTGTAAATATTTACTCTTTGCAAACCCTGGTGGTATTTTATTTTG
GCTTCATTTCAATCATTGAAGTATATTCTTATTGGAAATGTACTTTTGGATAAGTAGGGC
TAAGCCAGTTGGATCTCTGGTTGTCTAGTCATTGTCATAAGTAAACCTAGTAAAACCTTG
TTCTATTTTTCAATCATCAAAAAGTAATTATAAATACGTATTACAAACAAGTGGATGTTT
TTAATGACCAATTGAGTAAGAACATCCCTGTCTTAACTGGCCTAAATTTCTTCTGGTAGT
GTCAGTTCAACTTTCAGAAGTGCCACTTAAGGAAGTTTGATTTTTGTTTTTGTAATGCAC
TGTTTTTAATCTCTCTCTCTTTTTTTTTTTTTTTTTGGTTTTAAAAGCACAATCACTAAA
CTTTATTTGTAAACCATTGTAACTATTAACCTTTTTTGTCTTATTGAAAAAAAAAATGTT
GAGAAGCGTTTTTAACCTGTTTTGTTAATGCTCTATGTTTGTATTTGGAATATTTGAATA
ATGACAGATGGTGACGTAACATGCATACTTTATTGTGGGCCATGAACCAAATGGTTCTTA
CTTTTCCTGGACTTAAAGAAAAAAAGAGGTTTAAGTTTGTTGTGGCCAATGTCGAAACCT
ACAAGATTTCCTTAAAATCTCTAATAGAGGCATTACTTGCTTTCAATTGACAAATGATGC
CCTCTGACTAGTAGATTTCTATGATCCTTTTTTGTCATTTTATGAATATCATTGATTTTA
TAATTGGTGCTATTTGAAGAAAAAAATGTACATTTATTCATAGATAGATAAGTATCAGGT
CTGACCCCAGTGGAAAACAAAGCCAAACAAAACTGAACCACAAAAAAAAAGGCTGGTGTT
CACCAAAACCAAACTTGTTCATTTAGATAATTTGAAAAAGTTCCATAGAAAAGGCGTGCA
GTACTAAGGGAACAATCCATGTGATTAATGTTTTCATTATGTTCATGTAAGAAGCCCCTT
ATTTTTAGCCATAATTTTGCATACTGAAAATCCAATAATCAGAAAAGTAATTTTGTCACA
TTATTTATTAAAAATGTTCTCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_133631 unedited
TCAACATATGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCAAGACCGTGG
AGGCAGGAAACGGCACTACTGCGCTTCTGCCTCGGCTCTTTGTTGTTCGCTTTGGATGGT
TCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTGGGGCCGGAGAAGACAAACCTTGGAAT
TCTTCCTCTGCAAAAGTCTCTGAGATACTGACAAGCGTCCGGAAAGGTCGACGAGTAATT
GCCCTGAAAACTCTTGGCTAATTGACCCACGTTGCTTATATTAAGCCTTTGTGTGTGGTG
TGTGGCTTCATACATTTGGGGACCCTATTTCCACTCCCTCCTCTTGGCATGAGACTGTAT
ACAGGATCCACCCGAGGACAATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAA
TATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACCTCGCATTGTTGAAC
ACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAG
GCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAG
ATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG
TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACC
TTGGAGAGGCTGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCA
GACAAAACCCTTCGGATGTCATGGTTGCAGTANGAGAGCCTGCAGTAATGGAATGCCAAC
CTCNCACGAGCCATCCTGAGCACACCATTTCATGGAAGAAAGATGGCTCTCCACTGGGAT
GATAAGATGAAAGAAATACTATACGAGGAGGGAAGCTCATGATCACTTACACCCCGTAAA
ATGACGCTGCN
>OriGene 3' read for NM_133631 unedited
NCCATCCTGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTT
TTATTTGAGAACATTTTTAATAAATAATGTGACAAATTACTTTTCTGATTATTGGATTTT
CAGTATGCAAAATTATGGCTAAAAATAAGGGGCTTCTTACATGAACATAATGAAAACATT
AATCACATGGATTGTTCCCTTAGTACTGCACGCCTTTTCTATGGAACTTTTTCAAATTAT
CTAAATGAACAAGTTTGGTTTTGGTGAACACCAGCCTTTTTTTTTGTGGTTCAGTTTTGT
TTGGCTTTGTTTTCCACTGGGGTCAGACCTGATACTTATCTATCTATGAATAAATGTACA
TTTTTTTCTTCAAATAGCACCAATTATAAAATCAATGATATTCATAAAATGACAAAAAAG
GATCATAGAAATCTACTAGTCAGAGGGCATCATTTGTCAATTGAAAGCAAGTAATGCCTC
TATTAGAGATTTTAAGGAAATCTTGTAGGTTTCGACATTGGCCACAACAAACTTAAACCT
CTTTTTTTCTTTAAGTCCAGGAAAAGTAAGAACCATTTGGTTCATGGCCCACAATAAAGT
ATGCATGTTACGTCACCATCTGTCATTATTCAAATATTCCAAATACAAACATAGAGCATT
AACAAAACAGGTTAAAAACGCTTCTCAACATTTTTTTTTTCATTAAGACAAAAAAGGTTA
ATAGTTACAATTGGTTTACAAATAAAGTTTATGGAATGTGCTTTTAAAACCCAAAAAAAA
AAAAAAAGGAGAGAGAGTTGAAAAACCGTGCTTTTACAAAAACAANAATCAAAACTTCCT
TAATGGGCACCTTCTGAAAGTTGAACCGACCCTACCCGAAGAAAATTAGGGCCGTTAAAG
ACAAGGGATGTTCTTACTCAATTGGGGCTTAAAAAAAC
Restriction Sites NotI-NotI     
ACCN NM_133631
Insert Size 7300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133631.1, NP_598334.1
RefSeq Size 7475 bp
RefSeq ORF 4839 bp
Locus ID 6091
Cytogenetics 3p12.3
Domains ig, FN3
Protein Families Druggable Genome
Protein Pathways Axon guidance
Gene Summary 'Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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