ROBO1 (NM_133631) Human Untagged Clone

CAT#: SC109740

ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO1
• Synonyms: DUTT1; SAX3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_133631 edited
  GAATTCGGCACCAGGCAAGACCGTGGAGGCAGGAAACGGCACTACTGCGCTTCTGCCTCG
  GCTCTTTGTTGTTCGCTTTGGATGGTTCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTG
  GGGCCGGAGAAGACAAACCTTGGAATTCTTCCTCTGCAAAAGTCTCTGAGATACTGACAA
  GCGTCCGGAAAGGTCGACGAGTAATTGCCCTGAAAACTCTTGGCTAATTGACCCACGTTG
  CTTATATTAAGCCTTTGTGTGTGGTGTGTGGCTTCATACATTTGGGGACCCTATTTCCAC
  TCCCTCCTCTTGGCATGAGACTGTATACAGGATCCACCCGAGGACAATGATTGCGGAGCC
  CGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGA
  AGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACC
  TGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGG
  GGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAG
  TGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGATGAAGGAGT
  CTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGGA
  AGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGG
  AGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATG
  GAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAA
  GCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAA
  TATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATT
  TGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATTTAAATGTGA
  GGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCAA
  ATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGA
  CATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTAC
  TCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGT
  TGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGC
  TATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTC
  ATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGATC
  TGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGC
  ATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCC
  TGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAG
  TCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCG
  AATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGG
  TCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGA
  AGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATCCC
  TAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTG
  GCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCA
  TGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCAT
  TAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGG
  AATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAG
  TCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCA
  CAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAGTC
  TCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATC
  AGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCT
  CAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGC
  AGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGG
  TGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACC
  TCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGA
  AACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCCTT
  TCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGG
  GGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGA
  GGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGC
  AGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACA
  CCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTT
  TACCTTCACACCAACAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAG
  GCCTGGACTTCTCAACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCC
  TAATACTGGCAACAACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAA
  CAGCGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAA
  CCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGT
  GGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGG
  GCGTTTTGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTC
  AAACCTCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACC
  ACTGGGACAGCAGAAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAA
  GCTGAACAAAGATTATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATC
  ATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGG
  GAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAA
  CTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGA
  GTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGC
  AAGGATGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCC
  CCCTGTTCGGGGAGCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGC
  CACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGA
  GACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACC
  ACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGA
  TATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGAT
  GCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGA
  CCTGGAGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGA
  CAACATTTCCAGCGGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGC
  TGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACG
  GCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCAC
  AAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGC
  CAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCC
  ACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCT
  GGAAGTACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATC
  ATCAGACAGAAAAGGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGT
  TGACATGCGAACAAATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAA
  AGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCA
  AGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCC
  CAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAA
  TGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGA
  TAATAATGAAGAATTAGAGGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCT
  AATGTGAAAATCATCACTCAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAAT
  GTTCAGTGCAATCAGAGTGTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTT
  TAAAAACTTTATTGGGTTTTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTC
  CTATTCCCCTCGCTGTTGGAGCAAACCATTATACCTTACTTCCAGCAAGCAAAGTGCTTT
  GACTTCTTGCTTCAGTCATCAGCCAGCAAGAGGGAACAAAACTGTTCTTTTGCATTTTGC
  CGCTGAGATATGGCATTGCACTGCTTATATGCCAAGCTAATTTATAGCAAGATATTGATC
  AAATATAGAAAGTTGATATTCAACCTCACAAGGGCTCTCAAAGTATAATCTTTCTATAGC
  CAACTGCTAATGCAAATTAAAACATATTTCATTTTAACATGATTTCAAAATCAGTTTTTC
  ATACTACCCTTTGCTGGAAGAAACTAAAAATATAGCAAATGCAGAACCACAAACAATTCG
  AATGGGGTAGAAACATTGTAAATATTTACTCTTTGCAAACCCTGGTGGTATTTTATTTTG
  GCTTCATTTCAATCATTGAAGTATATTCTTATTGGAAATGTACTTTTGGATAAGTAGGGC
  TAAGCCAGTTGGATCTCTGGTTGTCTAGTCATTGTCATAAGTAAACCTAGTAAAACCTTG
  TTCTATTTTTCAATCATCAAAAAGTAATTATAAATACGTATTACAAACAAGTGGATGTTT
  TTAATGACCAATTGAGTAAGAACATCCCTGTCTTAACTGGCCTAAATTTCTTCTGGTAGT
  GTCAGTTCAACTTTCAGAAGTGCCACTTAAGGAAGTTTGATTTTTGTTTTTGTAATGCAC
  TGTTTTTAATCTCTCTCTCTTTTTTTTTTTTTTTTTGGTTTTAAAAGCACAATCACTAAA
  CTTTATTTGTAAACCATTGTAACTATTAACCTTTTTTGTCTTATTGAAAAAAAAAATGTT
  GAGAAGCGTTTTTAACCTGTTTTGTTAATGCTCTATGTTTGTATTTGGAATATTTGAATA
  ATGACAGATGGTGACGTAACATGCATACTTTATTGTGGGCCATGAACCAAATGGTTCTTA
  CTTTTCCTGGACTTAAAGAAAAAAAGAGGTTTAAGTTTGTTGTGGCCAATGTCGAAACCT
  ACAAGATTTCCTTAAAATCTCTAATAGAGGCATTACTTGCTTTCAATTGACAAATGATGC
  CCTCTGACTAGTAGATTTCTATGATCCTTTTTTGTCATTTTATGAATATCATTGATTTTA
  TAATTGGTGCTATTTGAAGAAAAAAATGTACATTTATTCATAGATAGATAAGTATCAGGT
  CTGACCCCAGTGGAAAACAAAGCCAAACAAAACTGAACCACAAAAAAAAAGGCTGGTGTT
  CACCAAAACCAAACTTGTTCATTTAGATAATTTGAAAAAGTTCCATAGAAAAGGCGTGCA
  GTACTAAGGGAACAATCCATGTGATTAATGTTTTCATTATGTTCATGTAAGAAGCCCCTT
  ATTTTTAGCCATAATTTTGCATACTGAAAATCCAATAATCAGAAAAGTAATTTTGTCACA
  TTATTTATTAAAAATGTTCTCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133631 unedited
  TCAACATATGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCAAGACCGTGG
  AGGCAGGAAACGGCACTACTGCGCTTCTGCCTCGGCTCTTTGTTGTTCGCTTTGGATGGT
  TCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTGGGGCCGGAGAAGACAAACCTTGGAAT
  TCTTCCTCTGCAAAAGTCTCTGAGATACTGACAAGCGTCCGGAAAGGTCGACGAGTAATT
  GCCCTGAAAACTCTTGGCTAATTGACCCACGTTGCTTATATTAAGCCTTTGTGTGTGGTG
  TGTGGCTTCATACATTTGGGGACCCTATTTCCACTCCCTCCTCTTGGCATGAGACTGTAT
  ACAGGATCCACCCGAGGACAATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAA
  TATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACCTCGCATTGTTGAAC
  ACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAG
  GCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAG
  ATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG
  TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACC
  TTGGAGAGGCTGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCA
  GACAAAACCCTTCGGATGTCATGGTTGCAGTANGAGAGCCTGCAGTAATGGAATGCCAAC
  CTCNCACGAGCCATCCTGAGCACACCATTTCATGGAAGAAAGATGGCTCTCCACTGGGAT
  GATAAGATGAAAGAAATACTATACGAGGAGGGAAGCTCATGATCACTTACACCCCGTAAA
  ATGACGCTGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133631 unedited
  NCCATCCTGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTATTTGAGAACATTTTTAATAAATAATGTGACAAATTACTTTTCTGATTATTGGATTTT
  CAGTATGCAAAATTATGGCTAAAAATAAGGGGCTTCTTACATGAACATAATGAAAACATT
  AATCACATGGATTGTTCCCTTAGTACTGCACGCCTTTTCTATGGAACTTTTTCAAATTAT
  CTAAATGAACAAGTTTGGTTTTGGTGAACACCAGCCTTTTTTTTTGTGGTTCAGTTTTGT
  TTGGCTTTGTTTTCCACTGGGGTCAGACCTGATACTTATCTATCTATGAATAAATGTACA
  TTTTTTTCTTCAAATAGCACCAATTATAAAATCAATGATATTCATAAAATGACAAAAAAG
  GATCATAGAAATCTACTAGTCAGAGGGCATCATTTGTCAATTGAAAGCAAGTAATGCCTC
  TATTAGAGATTTTAAGGAAATCTTGTAGGTTTCGACATTGGCCACAACAAACTTAAACCT
  CTTTTTTTCTTTAAGTCCAGGAAAAGTAAGAACCATTTGGTTCATGGCCCACAATAAAGT
  ATGCATGTTACGTCACCATCTGTCATTATTCAAATATTCCAAATACAAACATAGAGCATT
  AACAAAACAGGTTAAAAACGCTTCTCAACATTTTTTTTTTCATTAAGACAAAAAAGGTTA
  ATAGTTACAATTGGTTTACAAATAAAGTTTATGGAATGTGCTTTTAAAACCCAAAAAAAA
  AAAAAAAGGAGAGAGAGTTGAAAAACCGTGCTTTTACAAAAACAANAATCAAAACTTCCT
  TAATGGGCACCTTCTGAAAGTTGAACCGACCCTACCCGAAGAAAATTAGGGCCGTTAAAG
  ACAAGGGATGTTCTTACTCAATTGGGGCTTAAAAAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133631
• Insert Size: 7300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133631.1, NP_598334.1
• RefSeq Size: 7475 bp
• RefSeq ORF: 4839 bp
• Locus ID: 6091
• Cytogenetics: 3p12.3
• Domains: ig, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: 'Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.