S100A4 (NM_019554) Human Untagged Clone

CAT#: SC109749

S100A4 (untagged)-Human S100 calcium binding protein A4 (S100A4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S100A4
• Synonyms: 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109749 sequence for NM_019554 edited (data generated by NextGen Sequencing)
  ATGGCGTGCCCTCTGGAGAAGGCCCTGGATGTGATGGTGTCCACCTTCCACAAGTACTCG
  GGCAAAGAGGGTGACAAGTTCAAGCTCAACAAGTCAGAACTAAAGGAGCTGCTGACCCGG
  GAGCTGCCCAGCTTCTTGGGGAAAAGGACAGATGAAGCTGCTTTCCAGAAGCTGATGAGC
  AACTTGGACAGCAACAGGGACAACGAGGTGGACTTCCAAGAGTACTGTGTCTTCCTGTCC
  TGCATCGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCAGGAAG
  AAATGA
  
  Clone variation with respect to NM_019554.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019554 unedited
  CACCAGCTTTCTTGGTTTGATCCTGACTGCTGTCATGGCGTGCCCTCTGGAGAAGGCCCT
  GGATGTGATGGTGTCCACCTTCCACAAGTACTCGGGCAAAGAGGGTGACAAGTTCAAGCT
  CAACAAGTCAGAACTAAAGGAGCTGCTGACCCGGGAGCTGCCCAGCTTCTTGGGGAAAAG
  GACAGATGAAGCTGCTTTCCAGAAGCTGATGAGCAACTTGGACAGCAACAGGGACAACGA
  GGTGGACTTCCAAGAGTACTGTGTCTTCCTGTCCTGCATCGCCATGATGTGTAACGAATT
  CTTTGAAGGCTTCCCAGATAAGCAGCCCAGGAAGAAATGAAAACTCCTCTGATGTGGTTG
  GGGGGTCTGCCAGCTGGGGCCCTCCCTGTCGCCAGTGGGCACTTTTTTTTTTCCACCCTG
  GCTCCTTCAGACACGTGCTTGATGCTGAGCAAGTTCAATAAAGATTCTTGGAAGTTTTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019554 unedited
  TATGGCCGCGGCCGCATCTAGGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTAAAACTTCCAAAAATTTTTATTGAACTTGCTCAACATCAACCACGT
  GTCTGAAGGACCCAGGGGGGAAAAAAAAAGTGCCCACTGGCGACAGGGAGGGCCCCAGTT
  GGCAAACCCCCCAACCACATCAGAGGAGTTTTCATTTTTTCCTGGGCTGCTTATCTGGGA
  AGCCTTCAAAAAATTCGTTACACATCATGGCGATGCAGGACAGGAAAACCCAGTCCTTTT
  GGAAGTCCCCCTCGTTGCCCCTGTTGCTGCCCAAGTTGCTCATCACCCTTTGGAAAGCAG
  TTTCATCTGCCCTTTTCCCCAAAAAGCTGGGCAGTTCCCGGGTAAACAGTTCCTTTATTT
  CTGACTTGCGGAGCTTGAACTTGTCACCCTTTTTGCCCGAGTCCTTGGGGAAGGGGGACC
  CCCTCCCCCCCAGGCCCTTCTCAATAGGGCCCGCCCTTGCATCCCCCCCCGATCAACCCA
  ATAAACCTCGCCCCCACCTCCCGGCCCCCCCTATCGTTTCTTCCCTTCCCCCTTCCTACG
  CCCTACCCATCCCCCCCTTTTCACCCCCATTTTTCCCCCCCCCCCCCCCCCCCCCCTTTT
  CCCCCCCGGTCCCCCCCTTCAACCCTCTCGCACCCCTCTACCCTTTCTTTCCCCCTTACC
  CCCCCCTCCCGCCCCCCTTTTCCCCCCCTTCCCATCCCTTTCCCCCCCATCTTTCTTCAC
  CGTCTTCGCGTCTCCCCCCCCCTCTTCATCCCCCTTCCCCCGCGCTCCTCTTCTCCCTCT
  CACTTCTCCCCCCCCCTTCCTCCCCGCTCCCCCTTTCTTTTTACTTCCCCCCCCATTCCC
  CACCCCCCCCCCCCCCCTCCTCATTTTCCCTCTCCCGCCCTTCCCTCTCTCTATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019554
• Insert Size: 540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019554.1, NP_062427.1
• RefSeq Size: 507 bp
• RefSeq ORF: 306 bp
• Locus ID: 6275
• Cytogenetics: 1q21.3
• Domains: S_100, EFh
• Gene Summary: 'The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) represents the longer transcript. Both variants 1 and 2 encode the same isoform.