SNAP25 (NM_130811) Human Untagged Clone

CAT#: SC109782

SNAP25 (untagged)-Human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 2


  "NM_130811" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SNAP25
Synonyms bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_130811 edited
GAATTCGGCACGAGGGAACACAACCCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTC
ACTGACCCCCCAGCCCAGGCGCCCAGCCACTCCCCACCGCTACCATGGCCGAAGACGCAG
ACATGCGCAATGAGCTGGAGGAGATGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGC
TGGAAAGCACCCGTCGTATGCTGCAACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGA
CTTTGGTTATGTTGGATGAACAAGGAGAACAACTCGATCGTGTCGAAGAAGGCATGAACC
ATATCAACCAAGACATGAAGGAGGCTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTG
GCCTTTTCATATGTCCTTGTAACAAGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGG
GCAATAATCAGGACGGAGTGGTGGCCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGC
AGATGGCCATCAGTGGCGGCTTCATCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAA
TGGATGAAAACCTAGAGCAGGTGAGCGGCATCATCGGGAACCTCCGTCACATGGCCCTGG
ATATGGGCAATGAGATCGATACACAGAATCGCCAGATCGACAGGATCATGGAGAAGGCTG
ATTCCAACAAAACCAGAATTGATGAGGCCAACCAACGTGCAACAAAGATGCTGGGAAGTG
GTTAAGTGTGCCCACCCGTGTTCTCCTCCAAATGCTGTCGGGCAAGATAGCTCCTTCATG
CTTTTCTCATGGTATTATCTAGTAGGTCTGCACACATAACACACATCAGTCCACCCCCAT
TGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCT
CTCTTGGTCTCTTTCTTTCCAAAGGTTGTACATAGTGGTCATTTGGTGGCTCTAACTCCT
TGATGTCCTGAGTTTCATTTTTCATTTTCTCTCCTCGGTGGCATTTGCTGAATAACAACA
ATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACAGTATTGTTCTTGTAAAAC
TGTGACATTCCACAGAGTTACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAA
AATGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTGTCTTTATGATTTCATGATTAGACA
ATGTGGAATTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTATGCATG
AGAACTAAATAGATTTTTAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATAC
TGATGAGACAACACACACACACACAAAACAACAGCAACAACAACAGAACAACAACAAAGC
ATGCTCAGTATTGAGACACTGTCAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCA
CTTTTTTCCTGTCAATATATAGAGACTTCTAAATCATAATCATCCTTTTTTAAAAAAAAG
AATTTTAAAAAAGATGGATTTGACACACTCACCATTTAATCATTTCCAGCAAAATATATG
TTTGGCTGAAATTATGTCAAATGGATGTAATATAGGGTTTGTTTGCTGCTTTTGATGGCT
ACGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGATGTAAATTTTATAAGGCTGA
CTCTTACTAACCACCATTTCCCCTGTGGTTTGTTATCAGTACAATTCTTTGTTGCTTAAT
CTAGAGCTATGCACACCAAATTGCTGAGATGTTTAGTAGCTGATAAAGAAACCTTTTAAA
AAAATAATATAAATGAATGAAATATAAACTGTGAGATAAATATCATTATAGCATGTAATA
TTAAATTCCTCCTGTCTCCTCTGTCAGTTTGTGAAGTGATTGACATTTTGTAGCTAGTTT
AAAATTATTAAAAATTATAGACTCCAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_130811 unedited
TTCGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCCGAGGGAACACAAC
CCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGCCC
AGCCACTCCCCACCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGA
TGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCTGC
AACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGACTTTGGTTATGTTGGATGAACAAG
GAGAACAACTCGATCGTGTCGAAGAAGGCATGAACCATATCAACCAAGACATGAAGGAGG
CTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTGGCCTTTTCATATGTCCTTGTAACA
AGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGGAGTGGTGG
CCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCA
TCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGA
GCGGCATCATCGGGAACCTCCGTCACATGGCCCTGGATATGGGCAATGAGATCGATACAC
AGAATCGCCAGATCGACAGGATCATGGAGAAGGCTGATTCCAACAAAACCAGAATTGATG
AGGCCAACCAACGTGCAACANAGATGCTGGGAAGCGGCTAAGTGTGCCCACCCCGTGTTC
TCCTCCAAATGCCTGTCGGCAGATAGCTCCTTCATGCCTTTCCTCATGGATTATCTAGNA
GGGTCTGCCACATAACACCACATAGTCCCACCCCATTGTGAAAGTTGTCCTGTGTCATCC
GTCAGCTTTCCACCATACTTGTGGGCTTTTGTTCC
>OriGene 3' read for NM_130811 unedited
GCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTCTATAATTTTTAATAATT
TTAAACTAGCTACAAAATGTCAATCACTTCACAAACTGACAGAGGAGACAGGAGGAATTT
AATATTACATGCTATAATGATATTTATCTCACAGTTTATATTTCATTCATTTATATTATT
TTTTTAAAAGGTTTCTTTATCAGCTACTAAACATCTCAGCAATTTGGTGTGCATAGCTCT
AGATTAAGCAACAAAGAATTGTACTGATAACAAACCACAGGGGAAATGGTGGTTAGTAAG
AGTCAGCCTTATAAAATTTACATCCACACTGTTTCACAGCAAGATTGCTCTCTCCAAAAC
GTAGCCATCAAAAGCAGCAAACAAACCCTATATTACATCCATTTGACATAATTTCAGCCA
AACATATATTTTGCTGGAAATGATTAAATGGTGAGTGTGTCAAATCCATCTTTTTTAAAA
TTCTTTTTTTTAAAAAAGGATGATTATGATTTAGAAGTCTCTATATATTGACAGGAAAAA
AGTGACACTACTGCACTTTTGCTGGTATAACTTAATCTTGACAGTGTCTCAATACTGAGC
ATGCTTTGTTGNTGNTCTGTTGTTGTTGCTGTTGNTTTGTGTGTGTGTGTGTTGGCTCAT
CAGTATGCTACTGTCATGGAAAGTTTTTGTTAAGTANGAATCTAAAATCTATTTAGCTCT
CATGCATAAATGCATAAATCACATCACTTTTTAGTGCATGCCTGTTATGTATTNCCCATT
GCCTATCCATGAATCCTAAGACGGTAACAGCCCTGAGGAACCAAGACGCCCATTTTGGAG
ATTCAGACCTGACCTCAAAGACCGTGCAGTACCCTGTGAATGTACAGTTTACAGACCATA
CGTGGATGAAAGATCACACCATGAGCATCCTATTGTGTATTCCCAAGCCCCGNGAAAAAG
AAATGACCTCGCTCAGG
Restriction Sites NotI-NotI     
ACCN NM_130811
Insert Size 1960 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_130811.1, NP_570824.1
RefSeq Size 2053 bp
RefSeq ORF 621 bp
Locus ID 6616
Cytogenetics 20p12.2
Domains t_SNARE, SNAP-25
Protein Families Druggable Genome
Protein Pathways SNARE interactions in vesicular transport
Gene Summary 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) contains an alternate exon 5 as compared to transcript variant 1. This results in an isoform (SNAP25B) that is the same length, and contains the same N- and C-termini as isoform SNAP25A, but has 9 aa differences internally.

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