SNAP25 (NM_130811) Human Untagged Clone

CAT#: SC109782

SNAP25 (untagged)-Human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAP25
• Synonyms: bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_130811 edited
  GAATTCGGCACGAGGGAACACAACCCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTC
  ACTGACCCCCCAGCCCAGGCGCCCAGCCACTCCCCACCGCTACCATGGCCGAAGACGCAG
  ACATGCGCAATGAGCTGGAGGAGATGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGC
  TGGAAAGCACCCGTCGTATGCTGCAACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGA
  CTTTGGTTATGTTGGATGAACAAGGAGAACAACTCGATCGTGTCGAAGAAGGCATGAACC
  ATATCAACCAAGACATGAAGGAGGCTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTG
  GCCTTTTCATATGTCCTTGTAACAAGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGG
  GCAATAATCAGGACGGAGTGGTGGCCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGC
  AGATGGCCATCAGTGGCGGCTTCATCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAA
  TGGATGAAAACCTAGAGCAGGTGAGCGGCATCATCGGGAACCTCCGTCACATGGCCCTGG
  ATATGGGCAATGAGATCGATACACAGAATCGCCAGATCGACAGGATCATGGAGAAGGCTG
  ATTCCAACAAAACCAGAATTGATGAGGCCAACCAACGTGCAACAAAGATGCTGGGAAGTG
  GTTAAGTGTGCCCACCCGTGTTCTCCTCCAAATGCTGTCGGGCAAGATAGCTCCTTCATG
  CTTTTCTCATGGTATTATCTAGTAGGTCTGCACACATAACACACATCAGTCCACCCCCAT
  TGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCT
  CTCTTGGTCTCTTTCTTTCCAAAGGTTGTACATAGTGGTCATTTGGTGGCTCTAACTCCT
  TGATGTCCTGAGTTTCATTTTTCATTTTCTCTCCTCGGTGGCATTTGCTGAATAACAACA
  ATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACAGTATTGTTCTTGTAAAAC
  TGTGACATTCCACAGAGTTACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAA
  AATGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTGTCTTTATGATTTCATGATTAGACA
  ATGTGGAATTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTATGCATG
  AGAACTAAATAGATTTTTAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATAC
  TGATGAGACAACACACACACACACAAAACAACAGCAACAACAACAGAACAACAACAAAGC
  ATGCTCAGTATTGAGACACTGTCAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCA
  CTTTTTTCCTGTCAATATATAGAGACTTCTAAATCATAATCATCCTTTTTTAAAAAAAAG
  AATTTTAAAAAAGATGGATTTGACACACTCACCATTTAATCATTTCCAGCAAAATATATG
  TTTGGCTGAAATTATGTCAAATGGATGTAATATAGGGTTTGTTTGCTGCTTTTGATGGCT
  ACGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGATGTAAATTTTATAAGGCTGA
  CTCTTACTAACCACCATTTCCCCTGTGGTTTGTTATCAGTACAATTCTTTGTTGCTTAAT
  CTAGAGCTATGCACACCAAATTGCTGAGATGTTTAGTAGCTGATAAAGAAACCTTTTAAA
  AAAATAATATAAATGAATGAAATATAAACTGTGAGATAAATATCATTATAGCATGTAATA
  TTAAATTCCTCCTGTCTCCTCTGTCAGTTTGTGAAGTGATTGACATTTTGTAGCTAGTTT
  AAAATTATTAAAAATTATAGACTCCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130811 unedited
  TTCGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCCGAGGGAACACAAC
  CCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGCCC
  AGCCACTCCCCACCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGA
  TGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCTGC
  AACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGACTTTGGTTATGTTGGATGAACAAG
  GAGAACAACTCGATCGTGTCGAAGAAGGCATGAACCATATCAACCAAGACATGAAGGAGG
  CTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTGGCCTTTTCATATGTCCTTGTAACA
  AGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGGAGTGGTGG
  CCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCA
  TCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGA
  GCGGCATCATCGGGAACCTCCGTCACATGGCCCTGGATATGGGCAATGAGATCGATACAC
  AGAATCGCCAGATCGACAGGATCATGGAGAAGGCTGATTCCAACAAAACCAGAATTGATG
  AGGCCAACCAACGTGCAACANAGATGCTGGGAAGCGGCTAAGTGTGCCCACCCCGTGTTC
  TCCTCCAAATGCCTGTCGGCAGATAGCTCCTTCATGCCTTTCCTCATGGATTATCTAGNA
  GGGTCTGCCACATAACACCACATAGTCCCACCCCATTGTGAAAGTTGTCCTGTGTCATCC
  GTCAGCTTTCCACCATACTTGTGGGCTTTTGTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130811 unedited
  GCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTCTATAATTTTTAATAATT
  TTAAACTAGCTACAAAATGTCAATCACTTCACAAACTGACAGAGGAGACAGGAGGAATTT
  AATATTACATGCTATAATGATATTTATCTCACAGTTTATATTTCATTCATTTATATTATT
  TTTTTAAAAGGTTTCTTTATCAGCTACTAAACATCTCAGCAATTTGGTGTGCATAGCTCT
  AGATTAAGCAACAAAGAATTGTACTGATAACAAACCACAGGGGAAATGGTGGTTAGTAAG
  AGTCAGCCTTATAAAATTTACATCCACACTGTTTCACAGCAAGATTGCTCTCTCCAAAAC
  GTAGCCATCAAAAGCAGCAAACAAACCCTATATTACATCCATTTGACATAATTTCAGCCA
  AACATATATTTTGCTGGAAATGATTAAATGGTGAGTGTGTCAAATCCATCTTTTTTAAAA
  TTCTTTTTTTTAAAAAAGGATGATTATGATTTAGAAGTCTCTATATATTGACAGGAAAAA
  AGTGACACTACTGCACTTTTGCTGGTATAACTTAATCTTGACAGTGTCTCAATACTGAGC
  ATGCTTTGTTGNTGNTCTGTTGTTGTTGCTGTTGNTTTGTGTGTGTGTGTGTTGGCTCAT
  CAGTATGCTACTGTCATGGAAAGTTTTTGTTAAGTANGAATCTAAAATCTATTTAGCTCT
  CATGCATAAATGCATAAATCACATCACTTTTTAGTGCATGCCTGTTATGTATTNCCCATT
  GCCTATCCATGAATCCTAAGACGGTAACAGCCCTGAGGAACCAAGACGCCCATTTTGGAG
  ATTCAGACCTGACCTCAAAGACCGTGCAGTACCCTGTGAATGTACAGTTTACAGACCATA
  CGTGGATGAAAGATCACACCATGAGCATCCTATTGTGTATTCCCAAGCCCCGNGAAAAAG
  AAATGACCTCGCTCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130811
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130811.1, NP_570824.1
• RefSeq Size: 2053 bp
• RefSeq ORF: 621 bp
• Locus ID: 6616
• Cytogenetics: 20p12.2
• Domains: t_SNARE, SNAP-25
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate exon 5 as compared to transcript variant 1. This results in an isoform (SNAP25B) that is the same length, and contains the same N- and C-termini as isoform SNAP25A, but has 9 aa differences internally.