SNRPB (NM_003091) Human Untagged Clone

CAT#: SC109783

SNRPB (untagged)-Human small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNRPB
• Synonyms: CCMS; COD; Sm-B/B'; SmB/B'; SmB/SmB'; snRNP-B; SNRPB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109783 sequence for NM_003091 edited (data generated by NextGen Sequencing)
  ATGACGGTGGGCAAGAGCAGCAAGATGCTGCAGCATATTGATTACAGGATGAGGTGCATC
  CTGCAGGACGGCCGGATCTTCATTGGCACCTTCAAGGCTTTTGACAAGCACATGAATTTG
  ATCCTCTGTGACTGTGATGAGTTCAGAAAGATCAAGCCAAAGAACTCCAAACAAGCAGAA
  AGGGAAGAGAAGCGAGTCCTCGGTCTGGTGCTGCTGCGAGGGGAGAATCTGGTCTCAATG
  ACAGTAGAGGGACCTCCTCCCAAAGATACTGGTATTGCTCGAGTTCCACTTGCTGGAGCT
  GCCGGGGGCCCAGGGATCGGCAGGGCTGCTGGCAGAGGAATCCCAGCTGGGGTTCCCATG
  CCCCAGGCTCCTGCAGGACTTGCTGGGCCAGTCCGTGGGGTTGGCGGGCCATCCCAACAG
  GTGATGACCCCACAAGGAAGAGGTACTGTTGCAGCCGCTGCAGCTGCTGCCACAGCCAGT
  ATTGCCGGGGCTCCAACCCAGTACCCACCTGGCCGTGGGGGTCCTCCCCCACCTATGGGC
  CGAGGAGCACCCCCTCCAGGCATGATGGGCCCACCTCCTGGTATGAGACCTCCTATGGGT
  CCCCCAATGGGGATCCCCCCTGGAAGAGGGACTCCAATGGGCATGCCCCCTCCGGGAATG
  CGGCCTCCTCCCCCTGGGATGCGAGGCCTTCTTTGA
  
  Clone variation with respect to NM_003091.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003091 unedited
  TTTGTATCCGACTTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGGGGTCGGAA
  CTCCAGGCTAGTGAGCTGGACCGGAAGTAGGTTTCTACCCGACCGCATTTTACGTGGTGC
  TGCATTTCCGGTAGCGGCGGCGGGAAATCGGCTGTGGGAGAGAGGCTAGGCCTCTGAGGA
  GGCGAATCCGGCGGGTATCAGAGCCATCAGAACCGCCACCATGACGGTGGGCAAGAGCAG
  CAAGATGCTGCAGCATATTGATTACAGGATGAGGTGCATCCTGCAGGACGGCCGGATCTT
  CATTGGCACCTTCAAGGCTTTTGACAAGCACATGAATTTGATCCTCTGTGACTGTGATGA
  GTTCAGAAAGATCAAGCCAAAGAACTCCAAACAAGCAGAAAGGGAAGAGAAGCGAGTCCT
  CGGTCTGGTGCTGCTGCGAGGGGAGAATCTGGTCTCAATGACAGTAGAGGGACCTCCTCC
  CAAAGATACTGGTATTGCTCGAGTTCCACTTGCTGGAGCTGCCGGGGGCCCAGGGATCGG
  CAGGGCTGCTGGCAGAGGAATCCCAGCTGGGGTTCCCATGCCCCAGGCTCCTGCAGGACT
  TGCTGGGCCAGTCCGTGGGGTTGGCGGGCCATCCCAACAGGTGATGACCCCACAAGGAAG
  AGGTACTGTTGCAGCCGCTGCAGCTGCTGCCACAGCCAGTATTGCCGGGGCTCCAACCCA
  GTACCCACCTGCCGTGGNGGTCCTCCCCCACCTATGGCCGNAGAGCACCCCCTCCAGCAT
  GATGGCCCACCTCCTGNTTGAGACCTCCTATGGGCCCCCATGGGGATCCCCCTGNAGAGG
  GACTCATGGGCATGCCCCCTCCGGAATGCCGCCCTCTCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003091 unedited
  NNGGGTTTTAGCTATGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTT
  TAATGTTCTCTTAAAAGTTTATTATAAACCAGTTTCATAGGCCACAAGGAGATAAAAGGA
  CTATGTACAGCCTTACGGGAAACAGGCAGGGAGCTGAGGAGGGCCAAGATGAGTCTAGGG
  CCTTGGTGGGCGCATTCCCGGGGGAGGGGGCCCTGTAAGGGAAACCAGACAATCCCATGA
  GACTCCACGAACAACAGCATAAGAAACAAACAGGTCTGTGGTAACGTGGCCCTGAGGAAT
  CGAGCCCACGCCTCTGCGGAGCTACTTCCATACTCTGTGGCCAAGGGTCAAAGAAGGCCT
  CGCATCCCAGGGGGAGGAGGCCGCATTCCCGGAGGGGGCATGCCCATTGGAGTCCCTCTT
  CCAGGGGGGATCCCCATTGGGGGACCCATAGGAGGTCTCATACCAGGAGGTGGGCCCATC
  ATGCCTGGAGGGGGTGCTCCTCGGCCCATAGGTGGGGGAGGACCCCCACGGCCAGGTGGG
  TACTGTGTTGGAGCCCCTGCAATACTGGCTGTGGCAGCAGCTGCAGCGGCTGCAACAGTA
  CCTCTTCTTTGGGGGCCATCACCTGTTGGGATGGCCCGCCACCCCCACGGACTGGCCCAC
  CAAGTCCTGCAGGAGCCTGGGGCATGGGAACCCCAACTGGGATTTCTTTGCCAGAAGCCC
  TGCCGGACCCTGGGCCCCCGGTAGTTTCACCAAGTGGAACTCGCAACATACCATCTTCTT
  TGGGAAGAGGCTCTTCTACTGGCATTGAAACAGTATCTTCCCTGGAAGCAACCCCAAACG
  GAGACCCCCTTCCCTTCCCTTCTGCTTGTTTGAGCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003091
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003091.3, NP_003082.1
• RefSeq Size: 1153 bp
• RefSeq ORF: 696 bp
• Locus ID: 6628
• Cytogenetics: 20p13
• Domains: Sm
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome, Systemic lupus erythematosus
• Gene Summary: 'The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, that causes a frameshift. The resulting isoform (B) is shorter and has a distinct C-terminus as compared to isoform B'.