SNRPB (NM_003091) Human Untagged Clone

CAT#: SC109783

SNRPB (untagged)-Human small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 2


  "NM_003091" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SNRPB
Synonyms CCMS; COD; Sm-B/B'; SmB/B'; SmB/SmB'; snRNP-B; SNRPB1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109783 sequence for NM_003091 edited (data generated by NextGen Sequencing)
ATGACGGTGGGCAAGAGCAGCAAGATGCTGCAGCATATTGATTACAGGATGAGGTGCATC
CTGCAGGACGGCCGGATCTTCATTGGCACCTTCAAGGCTTTTGACAAGCACATGAATTTG
ATCCTCTGTGACTGTGATGAGTTCAGAAAGATCAAGCCAAAGAACTCCAAACAAGCAGAA
AGGGAAGAGAAGCGAGTCCTCGGTCTGGTGCTGCTGCGAGGGGAGAATCTGGTCTCAATG
ACAGTAGAGGGACCTCCTCCCAAAGATACTGGTATTGCTCGAGTTCCACTTGCTGGAGCT
GCCGGGGGCCCAGGGATCGGCAGGGCTGCTGGCAGAGGAATCCCAGCTGGGGTTCCCATG
CCCCAGGCTCCTGCAGGACTTGCTGGGCCAGTCCGTGGGGTTGGCGGGCCATCCCAACAG
GTGATGACCCCACAAGGAAGAGGTACTGTTGCAGCCGCTGCAGCTGCTGCCACAGCCAGT
ATTGCCGGGGCTCCAACCCAGTACCCACCTGGCCGTGGGGGTCCTCCCCCACCTATGGGC
CGAGGAGCACCCCCTCCAGGCATGATGGGCCCACCTCCTGGTATGAGACCTCCTATGGGT
CCCCCAATGGGGATCCCCCCTGGAAGAGGGACTCCAATGGGCATGCCCCCTCCGGGAATG
CGGCCTCCTCCCCCTGGGATGCGAGGCCTTCTTTGA

Clone variation with respect to NM_003091.3
>OriGene 5' read for NM_003091 unedited
TTTGTATCCGACTTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGGGGTCGGAA
CTCCAGGCTAGTGAGCTGGACCGGAAGTAGGTTTCTACCCGACCGCATTTTACGTGGTGC
TGCATTTCCGGTAGCGGCGGCGGGAAATCGGCTGTGGGAGAGAGGCTAGGCCTCTGAGGA
GGCGAATCCGGCGGGTATCAGAGCCATCAGAACCGCCACCATGACGGTGGGCAAGAGCAG
CAAGATGCTGCAGCATATTGATTACAGGATGAGGTGCATCCTGCAGGACGGCCGGATCTT
CATTGGCACCTTCAAGGCTTTTGACAAGCACATGAATTTGATCCTCTGTGACTGTGATGA
GTTCAGAAAGATCAAGCCAAAGAACTCCAAACAAGCAGAAAGGGAAGAGAAGCGAGTCCT
CGGTCTGGTGCTGCTGCGAGGGGAGAATCTGGTCTCAATGACAGTAGAGGGACCTCCTCC
CAAAGATACTGGTATTGCTCGAGTTCCACTTGCTGGAGCTGCCGGGGGCCCAGGGATCGG
CAGGGCTGCTGGCAGAGGAATCCCAGCTGGGGTTCCCATGCCCCAGGCTCCTGCAGGACT
TGCTGGGCCAGTCCGTGGGGTTGGCGGGCCATCCCAACAGGTGATGACCCCACAAGGAAG
AGGTACTGTTGCAGCCGCTGCAGCTGCTGCCACAGCCAGTATTGCCGGGGCTCCAACCCA
GTACCCACCTGCCGTGGNGGTCCTCCCCCACCTATGGCCGNAGAGCACCCCCTCCAGCAT
GATGGCCCACCTCCTGNTTGAGACCTCCTATGGGCCCCCATGGGGATCCCCCTGNAGAGG
GACTCATGGGCATGCCCCCTCCGGAATGCCGCCCTCTCCC
>OriGene 3' read for NM_003091 unedited
NNGGGTTTTAGCTATGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTT
TAATGTTCTCTTAAAAGTTTATTATAAACCAGTTTCATAGGCCACAAGGAGATAAAAGGA
CTATGTACAGCCTTACGGGAAACAGGCAGGGAGCTGAGGAGGGCCAAGATGAGTCTAGGG
CCTTGGTGGGCGCATTCCCGGGGGAGGGGGCCCTGTAAGGGAAACCAGACAATCCCATGA
GACTCCACGAACAACAGCATAAGAAACAAACAGGTCTGTGGTAACGTGGCCCTGAGGAAT
CGAGCCCACGCCTCTGCGGAGCTACTTCCATACTCTGTGGCCAAGGGTCAAAGAAGGCCT
CGCATCCCAGGGGGAGGAGGCCGCATTCCCGGAGGGGGCATGCCCATTGGAGTCCCTCTT
CCAGGGGGGATCCCCATTGGGGGACCCATAGGAGGTCTCATACCAGGAGGTGGGCCCATC
ATGCCTGGAGGGGGTGCTCCTCGGCCCATAGGTGGGGGAGGACCCCCACGGCCAGGTGGG
TACTGTGTTGGAGCCCCTGCAATACTGGCTGTGGCAGCAGCTGCAGCGGCTGCAACAGTA
CCTCTTCTTTGGGGGCCATCACCTGTTGGGATGGCCCGCCACCCCCACGGACTGGCCCAC
CAAGTCCTGCAGGAGCCTGGGGCATGGGAACCCCAACTGGGATTTCTTTGCCAGAAGCCC
TGCCGGACCCTGGGCCCCCGGTAGTTTCACCAAGTGGAACTCGCAACATACCATCTTCTT
TGGGAAGAGGCTCTTCTACTGGCATTGAAACAGTATCTTCCCTGGAAGCAACCCCAAACG
GAGACCCCCTTCCCTTCCCTTCTGCTTGTTTGAGCTTT
Restriction Sites NotI-NotI     
ACCN NM_003091
Insert Size 1250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003091.3, NP_003082.1
RefSeq Size 1153 bp
RefSeq ORF 696 bp
Locus ID 6628
Cytogenetics 20p13
Domains Sm
Protein Families Stem cell - Pluripotency
Protein Pathways Spliceosome, Systemic lupus erythematosus
Gene Summary 'The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, that causes a frameshift. The resulting isoform (B) is shorter and has a distinct C-terminus as compared to isoform B'.

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