SNRPN (NM_022806) Human Untagged Clone

CAT#: SC109786

SNRPN (untagged)-Human small nuclear ribonucleoprotein polypeptide N (SNRPN), transcript variant 3


  "NM_022806" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SNRPN
Synonyms HCERN3; PWCR; PWS; RT-LI; SM-D; sm-N; SMN; SNRNP-N; SNURF-SNRPN
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_022806, the custom clone sequence may differ by one or more nucleotides


ATGACTGTTGGCAAGAGTAGCAAGATGCTGCAGCACATTGACTATAGAATGAGATGTATCCTGCAAGATG
GCCGAATCTTCATTGGCACCTTTAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGA
GTTCAGAAAGATCAAGCCAAAGAATGCGAAGCAACCAGAGCGTGAAGAAAAGCGGGTTTTGGGTCTGGTG
TTGCTGCGTGGGGAGAACTTGGTATCCATGACTGTGGAGGGGCCACCCCCCAAAGATACTGGCATTGCTC
GGGTACCACTTGCTGGAGCTGCTGGAGGCCCTGGGGTTGGTAGGGCAGCTGGTAGAGGAGTACCAGCTGG
TGTGCCAATTCCCCAGGCCCCTGCTGGATTGGCAGGCCCTGTCCGAGGAGTTGGGGGACCATCCCAGCAG
GTAATGACTCCACAGGGAAGAGGCACTGTAGCAGCTGCTGCTGTTGCTGCGACTGCCAGTATTGCTGGAG
CCCCAACACAGTACCCACCAGGACGGGGCACTCCGCCCCCACCCGTCGGCAGAGCAACCCCACCTCCAGG
CATTATGGCTCCTCCACCTGGTATGAGACCACCCATGGGCCCACCAATTGGGCTTCCCCCTGCTCGAGGG
ACGCCAATAGGCATGCCGCCTCCGGGAATGAGACCCCCTCCACCAGGCATTAGAGGTCCACCTCCCCCAG
GAATGCGTCCACCAAGACCTTAG


>OriGene 5' read for NM_022806 unedited
GGTTCATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAAGGGATCG
CTTACACCTGAGACGAACTACAGAACAGCACGTACCAGAGGTGGAAGTCCAAGTCAAACG
CAGAAGGACTGCCTCACTGAGCAACCAAGAGTGTCAGTTGTACCCGAGGCGTTCTCAGCA
GCAGCAAGTACCTGTGGTGGATTTCCAGGCTGAACTGAGGCAGGCATTCTTAGCTGAGAC
ACCAAGAGGTGGTTAAAGCCATATTGGAGTAGCGAGGAATCTGATTCCAAGCAAAAACCA
GGCTCCATCTACTCTTTGAAGCTTCTGCCCAGCTTGCATTGTTTCTAGGAGAACCTGCGT
CATACCTTTATCTATAGCCTTCCCCTAGGTCTTCAGAAGCATCAAGTTTTAACTGTGGAC
ATTGGATTTGGTGGAACAGCAATCATGACTGTTGGCAAGAGTAGCAAGATGCTGCAGCAC
ATTGACTATAGAATGAGATGTATCCTGCAAGATGGCCGAATCTTCATTGGCACCTTTAAG
GCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAGTTCAGAAAGATCAAG
CCAAAGAATGCGAAGCAACCAGAGCGTGAAGAAAAGCGGGTTTTGGGTCTGGTGTTGCTG
CGTGGNGAGAACTTGGTATCCATGACTGTGGAGGGGCCACCCCCCAAAGATACTGGCATT
GCTCGGGTACCACTTGCTGGAGCTGCTGGAGGCCCCTGGGNTNGGTAGGGCAGCTGTAAA
GGAGTACCAGCTGGTGTGCCAATTNCCCCAGCCCCTGCTGGATTGNCANGCCCTGTCCGA
GAGTTGGGGGACATCCCCAGCAGTATGACTCCACAGGANAAGCACTGTAACNCTGCTGCT
NGTGCTGN
>OriGene 3' read for NM_022806 unedited
CGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAATTGCTCTATGCATTTATTATTAG
CTATTATTAATGCAGAATGAGGGAACAAAAAGCTCACAAACACTCTACACAATTTCACAA
GACGCATTGCAGGGGAAAAAGTGACTGAGATGGATCAACAGTATGCTAAGGTCTTGGTGG
ACGCATTCCTGGGGGAGGTGGACCTCTAATGCCTGGTGGAGGGGGTCTCATTCCCGGAGG
CGGCATGCCTATTGGCGTCCCTCGAGCAGGGGGAAGCCCAATTGGTGGGCCCATGGGTGG
TCTCATACCAGGTGGAGGAGCCATAATGCCTGGAGGTGGGGTTGCTCTGCCGACGGGTGG
AGGCGGAGTGCCCCGTCCTGGTGGGTACTGTGTTGGGGCTCCAGCAATACTGGCAGTCGC
AGCAACAGCAGCAGCTGCTACAGTGCCTCTTCCCTGTGGAGTCATTACCTGCTGGGATGG
TCCCCCAACTCCTCGGACAGGGCCTGCCAATCCAGCAGGGGCCTGGGGAATTGGCACACC
AGCTGGTACTCCTCTACCAGCTGCCCTACCAACCCCAGGGCCTCCAGCAGCTCCAGCAAG
TGGTACCCGAGCAATGCCAGTATCTTTGGAGGGTGGCCCCTNCACAGTCATGGATACCAA
GTTCTCCCCACGCAGCATCACCAGACCCATTACCCGCTTTTCTTCACGCTCTGGTTGCTT
CGCATTCTTTGGCTTGATCTTTCTGAACTCATCANCATCACCAGAGATCAAATTCTATGN
CTGTCAAAAGCTTAAAGTGCAATGAAGATTCGCCATCTGCAGATCATCTATCTATATCAT
GNGCTGCAGATCTGCTACTCTTGCACAGNATGATGCTGTTCACAAATCATGCCACATTAA
ACTGNAGCTCTGAGACTAGGGAAGCTANATAAGTTGACCCAGTCTCAAACATGCAGCTGG
CAAGCTCAAGATTATGAC
Restriction Sites NotI-NotI     
ACCN NM_022806
Insert Size 1330 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022806.2, NP_073717.1
RefSeq Size 1616 bp
RefSeq ORF 723 bp
Locus ID 6638
Cytogenetics 15q11.2
Domains Sm
Protein Families Stem cell - Pluripotency
Gene Summary 'This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]'
Transcript Variant: This variant (3) represents use of an alternate upstream promoter compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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