Staufen (STAU1) (NM_017453) Human Untagged Clone

CAT#: SC109798

STAU1 (untagged)-Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAU1
• Synonyms: PPP1R150; STAU
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017453, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCAAGTTCAAGTGCAAGTTCAGAACCCATCTGCTGCTCTCTCAGGGAGCCAAATACTGAACAAGA
  ACCAGTCTCTTCTCTCACAGCCTTTGATGAGTATTCCTTCTACTACTAGCTCTCTGCCCTCTGAAAATGC
  AGGTAGACCCATTCAAAACTCTGCTTTACCCTCTGCATCTATTACATCCACCAGTGCAGCTGCAGAAAGC
  ATAACCCCTACTGTAGAACTAAATGCACTGTGCATGAAACTTGGAAAAAAACCAATGTATAAGCCTGTTG
  ACCCTTACTCTCGGATGCAGTCCACCTATAACTACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTT
  TTACCCATTTCCAGTTCCACCTTTACTTTATCAAGTGGAACTTTCTGTGGGAGGACAGCAATTTAATGGC
  AAAGGAAAGACAAGACAGGCTGCGAAACACGATGCTGCTGCCAAAGCGTTGAGGATCCTGCAGAATGAGC
  CCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAGAATCCGAAGAAGAAAATCTCAATAAATCTGAAATAAG
  TCAAGTGTTTGAGATTGCACTTAAACGGAACTTGCCTGTGAATTTCGAGGTGGCCCGGGAGAGTGGCCCA
  CCCCACATGAAGAACTTTGTGACCAAGGTTTCGGTTGGGGAGTTTGTGGGGGAAGGTGAAGGGAAAAGCA
  AGAAGATTTCAAAGAAAAATGCCGCCATAGCTGTTCTTGAGGAGCTGAAGAAGTTACCGCCCCTGCCTGC
  AGTTGAACGAGTAAAGCCTAGAATCAAAAAGAAAACAAAACCCATAGTCAAGCCACAGACAAGCCCAGAA
  TATGGCCAGGGGATCAATCCGATTAGCCGACTGGCCCAGATCCAGCAGGCAAAAAAGGAGAAGGAGCCAG
  AGTACACGCTCCTCACAGAGCGAGGCCTCCCGCGCCGCAGGGAGTTTGTGATGCAGGTGAAGGTTGGAAA
  CCACACTGCAGAAGGAACGGGCACCAACAAGAAGGTGGCCAAGCGCAATGCAGCCGAGAACATGCTGGAG
  ATCCTTGGTTTCAAAGTCCCGCAGGCGCAGCCCACCAAACCCGCACTCAAGTCAGAGGAGAAGACACCCA
  TAAAGAAACCAGGGGATGGAAGAAAAGTAACCTTTTTTGAACCTGGCTCTGGGGATGAAAATGGGACTAG
  TAATAAAGAGGATGAGTTCAGGATGCCTTATCTAAGTCATCAGCAGCTGCCTGCTGGAATTCTTCCCATG
  GTGCCCGAGGTCGCCCAGGCTGTAGGAGTTAGTCAAGGACATCACACCAAAGATTTTACCAGGGCAGCTC
  CGAATCCTGCCAAGGCCACGGTAACTGCCATGATAGCCCGAGAGTTGTTGTATGGGGGCACCTCGCCCAC
  AGCCGAGACCATTTTAAAGAATAACATCTCTTCAGGCCACGTACCCCATGGACCTCTCACGAGACCCTCT
  GAGCAACTGGACTATCTTTCCAGAGTCCAGGGATTCCAGGTTGAATACAAAGACTTCCCCAAAAACAACA
  AGAACGAATTTGTATCTCTTATCAATTGCTCCTCTCAGCCACCTCTGATCAGCCATGGTATCGGCAAGGA
  TGTGGAGTCCTGCCATGATATGGCTGCGCTGAACATCTTAAAGTTGCTGTCTGAGTTGGACCAACAAAGT
  ACAGAGATGCCAAGAACAGGAAACGGACCAATGTCTGTGTGTGGGAGGTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017453 unedited
  NTGGTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGCCGC
  GTCTCTCTCGGCTCCCGCTTCCTTTGACCGCCTCCCCCCCCCGGCCCGGCGGCGCCCGCC
  TCCTCCACGGCCACTCCGCCTCTTCCCTCCCTTCGTCCCTTCTTCCTCTCCCTTTTTTCC
  TTCTTCCTTCCCCTCCTCGCCGCCACCGCCCAGGACCGCCGGCCGGGGGACGAGCTCGGA
  GCAGCAGCCAGAGTTTATTAACCACTTAACCTCTCAGAACTGAACAAAGACAACATTGTT
  CCTGGAACGCCCTCTTTTTAAAAAAGGTAGAACTTTAGACTTCATAGCACTGAATTAACC
  TGCACTGAAAGCTGTTTACCTGCATTTGTTCACTTTTGTTGAAAGTGACCATGTCTCAAG
  TTCAAGTGCAAGTTCAGAACCCATCTGCTGCTCTCTCAGGGAGCCAAATACTGAACAAGA
  ACCAGTCTCTTCTCTCACAGCCTTTGATGAGTATTCCTTCTACTACTAGCTCTCTGCCCT
  CTGAAAATGCAGGTAGACCCATTCAAAACTCTGCTTTACCCTCTGCATCTATTACATCCA
  CCAGTGCAGCTGCAGAAAGCATAACCCCTACTGTAGAACTAAATGCACTGTGCATGAAAC
  TTGGAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTCGGATGCAGTCCACCTATA
  ACTACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTTACCCATTTCCAGTTCCAC
  CTTTACTTTATCAAGTGGAACTTTCTGTGGGAAGACCAGCATTTAATGGCANAGGNAAAG
  ACAGACAGGCTGCGAAACACGATGCTGCTNGCCAAAGCGTTGAGGATCCTGCAGAATGAG
  CCCCTGCCANNANNAGCTGNNAGTGAATGGNANANAATCNCGAGAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017453 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAATTAGTAG
  CTGTTTATTGATCAATGGTTTGATATAAAGTTATTTCAGATCTTCAAACTTTTGCCCAGA
  TGGAATCACAAGCATTACAAAGTTTTTTTCTTAAAAATAAAAAAAGGATAGGGGCAAGTT
  GGGAGGGGACCAACCTAGCAGTAGTGGCATTTGAGAATAAATTAACAAAAAAATTTAGTA
  TTACCATTTATTGATGACAAACACTTAAGTTTTACTTACATTCCATGGGGAGAAAAATTC
  CAGCGTAAACAATGAATGGAAGCAGTACTTAACTCGCAGGGCTACCAGGCTTTCCATACG
  GACCACACGCAGAGCCTCAGTGCACACACTTCTGTGTACAGTAACACAACATCAAAAGCA
  ACACAGCTGTATACAGAAACGTAGGTCATTCTTTTCAGCCCTAATGGAGATGTAATTAAC
  AGTATCGAGCACTCTGGAAAATCACTCTGCAGGTTTATATGGACTACATGGAGATCATAT
  CCTGTAGTGTAGTGAAAGCTAAGTCCTCAAGAGCCATATGTATAGATACACAATGTTTTT
  TAATAATCTTTAAAACAGAGATCAAAGTTCATTTAAGTCCTGTTTGCATTAACAAAAATA
  AAAATGAAATAAAAATGGAACCAAATGATCATCTAAAGTTTAAAATTCCTAAATTGTCCA
  ATTTATACAACTGTGGGAGACTTATTCAAGGTTTTTGAAGTCCAAGACTGTTTCANCTGA
  ACCAAAGGGCACACAATTTGCATCACTGAAACTGTCCTGGGGTAGTCAATGAAAAAACAA
  TGTCTAACCACATACCCTGGATATGATCTTTGTGCGGTTAAATATTGAANAAGCAGTCCT
  AAAACGTTTTACAAATGGGCCTATTAACTAGAACAATTGAAAATTCTNNAAGAAGATTCT
  TTTAGGGGCGTATCAATCACAGAGAATTAGGAACCTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017453
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017453.1, NP_059347.1
• RefSeq Size: 3688 bp
• RefSeq ORF: 1734 bp
• Locus ID: 6780
• Cytogenetics: 20q13.13
• Domains: DSRM
• Gene Summary: 'Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]'
  Transcript Variant: This variant (T3) differs in the 5' UTR and the 5' coding region, and uses an upstream translation start site compared to variant T2. The encoded isoform (b) has a longer and distinct N-terminus compared to isoform a. Both variants T3 and T7 encode the same isoform (b).