Staufen (STAU1) (NM_017453) Human Untagged Clone

CAT#: SC322648

STAU1 (untagged)-Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAU1
• Synonyms: PPP1R150; STAU
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322648 AACCACTTAACCTCTCAGAACTGAACAAAGACAACATTGTTCCTGGAACGCCCTCTTTTT
  AAAAAAAGGTAGAACTTTAGACTTCATAGCACTGAATTAACCTGCACTGAAAGCTGTTTA
  CCTGCATTTGTTCACTTTTGTTGAAAGTGACCATGTCTCAAGTTCAAGTGCAAGTTCAGA
  ACCCATCTGCTGCTCTCTCAGGGAGCCAAATACTGAACAAGAACCAGTCTCTTCTCTCAC
  AGCCTTTGATGAGTATTCCTTCTACTACTAGCTCTCTGCCCTCTGAAAATGCAGGTAGAC
  CCATTCAAAACTCTGCTTTACCCTCTGCATCTATTACATCCACCAGTGCAGCTGCAGAAA
  GCATAACCCCTACTGTAGAACTAAATGCACTGTGCATGAAACTTGGAAAAAAACCAATGT
  ATAAGCCTGTTGACCCTTACTCTCGGATGCAGTCCACCTATAACTACAACATGAGAGGAG
  GTGCTTATCCCCCGAGGTACTTTTACCCATTTCCAGTTCCACCTTTACTTTATCAAGTGG
  AACTTTCTGTGGGAGGACAGCAATTTAATGGCAAAGGAAAGACAAGACAGGCTGCGAAAC
  ACGATGCTGCTGCCAAAGCGTTGAGGATCCTGCAGAATGAGCCCCTGCCAGAGAGGCTGG
  AGGTGAATGGAAGAGAATCCGAAGAAGAAAATCTCAATAAATCTGAAATAAGTCAAGTGT
  TTGAGATTGCACTTAAACGGAACTTGCCTGTGAATTTCGAGGTGGCCCGGGAGAGTGGCC
  CACCCCACATGAAGAACTTTGTGACCAAGGTTTCGGTTGGGGAGTTTGTGGGGGAAGGTG
  AAGGGAAAAGCAAGAAGATTTCAAAGAAAAATGCCGCCATAGCTGTTCTTGAGGAGCTGA
  AGAAGTTACCGCCCCTGCCTGCAGTTGAACGAGTAAAGCCTAGAATCAAAAAGAAAACAA
  AACCCATAGTCAAGCCACAGACAAGCCCAGAATATGGCCAGGGGATCAATCCGATTAGCC
  GACTGGCCCAGATCCAGCAGGCAAAAAAGGAGAAGGAGCCAGAGTACACGCTCCTCACAG
  AGCGAGGCCTCCCGCGCCGCAGGGAGTTTGTGATGCAGGTGAAGGTTGGAAACCACACTG
  CAGAAGGAACGGGCACCAACAAGAAGGTGGCCAAGCGCAATGCAGCCGAGAACATGCTGG
  AGATCCTTGGTTTCAAAGTCCCGCAGGCGCAGCCCACCAAACCCGCACTCAAGTCAGAGG
  AGAAGACACCCATAAAGAAACCAGGGGATGGAAGAAAAGTAACCTTTTTTGAACCTGGCT
  CTGGGGATGAAAATGGGACTAGTAATAAAGAGGATGAGTTCAGGATGCCTTATCTAAGTC
  ATCAGCAGCTGCCTGCTGGAATTCTTCCCATGGTGCCCGAGGTCGCCCAGGCTGTAGGAG
  TTAGTCAAGGACATCACACCAAAGATTTTACCAGGGCAGCTCCGAATCCTGCCAAGGCCA
  CGGTAACTGCCATGATAGCCCGAGAGTTGTTGTATGGGGGCACCTCGCCCACAGCCGAGA
  CCATTTTAAAGAATAACATCTCTTCAGGCCACGTACCCCATGGACCTCTCACGAGACCCT
  CTGAGCAACTGGACTATCTTTCCAGAGTCCAGGGATTCCAGGTTGAATACAAAGACTTCC
  CCAAAAACAACAAGAACGAATTTGTATCTCTTATCAATTGCTCCTCTCAGCCACCTCTGA
  TCAGCCATGGTATCGGCAAGGATGTGGAGTCCTGCCATGATATGGCTGCGCTGAACATCT
  TAAAGTTGCTGTCTGAGTTGGACCAACAAAGTACAGAGATGCCAAGAACAGGAAACGGAC
  CAATGTCTGTGTGTGGGAGGTGCTGAACCTTTTCTGGCCATGAACCATTATAAAATCCCA
  ACATATATACTGAAAATACTGAAACTGCTTTGAAAATTTGGAATTTCTGATACCTCCAGT
  GGGCCGAGAGACACGGTGGGTAAAGGATGTGGGCAGCAGCAGGGAAGACAACAGAAACAC
  AAGGAGGCGGCTGTGGCCGGGCTGGACTGTGCGGGGGTTTGTTGTGATGGCCACTCGGTG
  ACCTGGCGGTCCCTACGCAATAGCAGCTGCCTGTGGGGAAGAGGGGCTGCCCAGCCAGCT
  GGTTCTCCCGGGACACCAGCAGATCCACACCCTGGGCACCTCCGTGTTTGGTCTTTTTTT
  TCCCCTGTGTGAAAGAAGAAACGGCACGACCCCTTCTCAAGCTGGCTCACTCAGACACAT
  TGGGACAAACCCTGGACAGCCATGCCAGAGAGAGGCCTTTGACCGGCCCCAGAGCTAAAA
  GCACCAGAGAAAATCAAATGCTTCCTACTCAGCGTGACCCAACTTTTCTAGTGTGCCACG
  GCCCCACCACCTCCTGCAGTACCCACACCATCACCACTGCTTTCTCTTCCAACAGTGATC
  TGTATTCTTAGTTTCATTATTTTCTTTTGATTGATATGACACTATATAAAATTTTCATTT
  GAGAATTTCTCAATTGTATCTAGTTAAATAGCACAGTTTGGAAACTTGTCTGAGACTGAC
  TTTATCAATAATCTAACCGACAAAGATCATATCCATGTGTATGTGGTTAGACATTTTTAT
  TTCATTGACTAACCCAGGACAGTTTCAGTGATGCAAATTGTGTGCCCTCTGGTTCAGCTG
  AAACAGTCCTGGACTTTCAAAAACCTTGAATAAGTCTCCCACAGTTGTATAAATTGGACA
  ATTTAGGAATTTTAAACTTTAGATGATCATTTGGTTCCATTTTTATTTCATTTTTATTTT
  TGTTAATGCAAACAGGACTTAAATGAACTTTGATCTCTGTTTTAAAGATTATTAAAAAAC
  ATTGTGTATCTATACATATGGCTCTTGAGGACTTAGCTTTCACTACACTACAGGATATGA
  TCTCCATGTAGTCCATATAAACCTGCAGAGTGATTTTCCAGAGTGCTCGATACTGTTAAT
  TACATCTCCATTAGGGCTGAAAAGAATGACCTACGTTTCTGTATACAGCTGTGTTGCTTT
  TGATGTTGTGTTACTGTACACAGAAGTGTGTGCACTGAGGCTCTGCGTGTGGTCCGTATG
  GAAAGCCTGGTAGCCCTGCGAGTTAAGTACTGCTTCCATTCATTGTTTACGCTGGAATTT
  TTCTCCCCATGGAATGTAAGTAAAACTTAAGTGTTTGTCATCAATAAATGGTAATACTAA
  AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_017453
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017453.2, NP_059347.2
• RefSeq Size: 3688 bp
• RefSeq ORF: 1734 bp
• Locus ID: 6780
• Cytogenetics: 20q13.13
• Domains: DSRM
• Gene Summary: 'Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]'
  Transcript Variant: This variant (T3) differs in the 5' UTR and the 5' coding region, and uses an upstream translation start site compared to variant T2. The encoded isoform (b) has a longer and distinct N-terminus compared to isoform a. Both variants T3 and T7 encode the same isoform (b).