Staufen (STAU1) (NM_017454) Human Untagged Clone

CAT#: SC109799

STAU1 (untagged)-Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAU1
• Synonyms: PPP1R150; STAU
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017454, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACTTGGAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTCGGATGCAGTCCACCTATAACT
  ACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTTACCCATTTCCAGTTCCACCTTTACTTTATCA
  AGTGGAACTTTCTGTGGGAGGACAGCAATTTAATGGCAAAGGAAAGACAAGACAGGCTGCGAAACACGAT
  GCTGCTGCCAAAGCGTTGAGGATCCTGCAGAATGAGCCCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAG
  AATCCGAAGAAGAAAATCTCAATAAATCTGAAATAAGTCAAGTGTTTGAGATTGCACTTAAACGGAACTT
  GCCTGTGAATTTCGAGGTGGCCCGGGAGAGTGGCCCACCCCACATGAAGAACTTTGTGACCAAGGTTTCG
  GTTGGGGAGTTTGTGGGGGAAGGTGAAGGGAAAAGCAAGAAGATTTCAAAGAAAAATGCCGCCATAGCTG
  TTCTTGAGGAGCTGAAGAAGTTACCGCCCCTGCCTGCAGTTGAACGAGTAAAGCCTAGAATCAAAAAGAA
  AACAAAACCCATAGTCAAGCCACAGACAAGCCCAGAATATGGCCAGGGGATCAATCCGATTAGCCGACTG
  GCCCAGATCCAGCAGGCAAAAAAGGAGAAGGAGCCAGAGTACACGCTCCTCACAGAGCGAGGCCTCCCGC
  GCCGCAGGGAGTTTGTGATGCAGGTGAAGGTTGGAAACCACACTGCAGAAGGAACGGGCACCAACAAGAA
  GGTGGCCAAGCGCAATGCAGCCGAGAACATGCTGGAGATCCTTGGTTTCAAAGTCCCGCAGGCGCAGCCC
  ACCAAACCCGCACTCAAGTCAGAGGAGAAGACACCCATAAAGAAACCAGGGGATGGAAGAAAAGTAACCT
  TTTTTGAACCTGGCTCTGGGGATGAAAATGGGACTAGTAATAAAGAGGATGAGTTCAGGATGCCTTATCT
  AAGTCATCAGCAGCTGCCTGCTGGAATTCTTCCCATGGTGCCCGAGGTCGCCCAGGCTGTAGGAGTTAGT
  CAAGGACATCACACCAAAGATTTTACCAGGGCAGCTCCGAATCCTGCCAAGGCCACGGTAACTGCCATGA
  TAGCCCGAGAGTTGTTGTATGGGGGCACCTCGCCCACAGCCGAGACCATTTTAAAGAATAACATCTCTTC
  AGGCCACGTACCCCATGGACCTCTCACGAGACCCTCTGAGCAACTGGACTATCTTTCCAGAGTCCAGGGA
  TTCCAGGTTGAATACAAAGACTTCCCCAAAAACAACAAGAACGAATTTGTATCTCTTATCAATTGCTCCT
  CTCAGCCACCTCTGATCAGCCATGGTATCGGCAAGGATGTGGAGTCCTGCCATGATATGGCTGCGCTGAA
  CATCTTAAAGTTGCTGTCTGAGTTGGACCAACAAAGTACAGAGATGCCAAGAACAGGAAACGGACCAATG
  TCTGTGTGTGGGAGGTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017454 unedited
  NNNNTGTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACCAGAAACAA
  CATTGTTCCTGGAACGCCCTCTTTTTAAAAAAGAAAGCATAACCCCTACTGTAGAACTAA
  ATGCACTGTGCATGAAACTTGAAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTC
  GGATGCAGTCCACCTATAACTACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTT
  ACCCATTTCCAGTTCCACCTTTACTTTATCAAGTGGAACTTTCTGTGGGAGGACAGCAAT
  TTAATGGCAAAGGAAAGACAAGACAGGCTGCGAAACACGATGCTGCTGCCAAAGCGTTGA
  GGATCCTGCAGAATGAGCCCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAGAATCCGAAG
  AAGAAAATCTCAATAAATCTGAAATAAGTCAAGTGTTTGAGATTGCACTTAAACGGAACT
  TGCCTGTGAATTTCGAGGTGGCCCGGGAGAGTGGCCCACCCCACATGAAGAACTTTGTGA
  CCAAGGTTTCGGTTGGGGAGTTTGGGGGAGGTGGAAAGGGGAAAAAAGCAGAAGATTTCA
  NAGAAAAATGCCGCATTAGCTGTTCTTGAGGAGCTGAAGAAGNTACCGCCCCTGCCTGCA
  GNTGAACGAGTAAAGCCTAGAATCAAAAAGAAACANAACCATAGTCAAGCCACAGACAAG
  CCCAGAATATGGCCCAGGGGATCAATCCGAATAGCCGACTGGCCCAGATCCAGCAGGCAA
  AAAGGAAGAAGAGCCAGAGTCACGCTTCTCCAGAGCGAGCCTCCGGCGAAGGAAATTTTG
  AAGCAGGGAAAAGTTTGAAACCACCTGGCAAAGGACGGGCACACAGAAAGGTGGCAACGC
  ATGCACCGAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017454 unedited
  NTTACTCTGNACCGCGNCCGCAATCTAGNGATCGAGTTTTTTTTTTTTTTTTTTTTTTAC
  ATTAGAAGCTGTTTATTGATCAATGGTTTGATATAAAGTTATTTCAAATCTTCAAACTTT
  TGCCCAGATGGAATCACAAGCATTACAAAGTTTTTTCTTAAAAATAAAAAAAGGATAGGG
  GCAAGTTGGGAGGGGACCAACCTAGCAGTAGTGGCATTTGAGAATAAATTAACAAAAAAA
  TTTAGTATTACCATTTATTGATGACAAACACTTAAGTTTTACTTACATTCCATGGGGAGA
  AAAATTCCAGCGTAAACAATGAATGGAAGCAGTACTTAACTCGCAGGGCTACCAGGCTTT
  CCATACGGACCACACGCAGAGCCTCAGTGCACACACTTTTGTGTACAGTAACACAACATC
  AAAAGCAACACAGCTGTTTACAGAAACGTAGGTCATTCTTTTCAGCCCTAATGGAGATGT
  AATTAACAGTATCGAGCACTCTGGAAAATCACTCTGCAGGTTTATATGGACTACATGGCG
  ATCATATCCTGTAGGGTACTGAAAGCTAAGTCCTCAAGACCCTTATGTTTAGATACACAC
  AGTTTTTTAATAATCTTTAAAACAGAAAATCAAACGTCTTTTAACGTCCCTGTCTGGCCC
  TNTCCACAATCTCAAATGCAACTAAAAATGGAACCCAAATTGATCTCCTTAACGTTTTAA
  ATCTCCTCAAATTGTTCCAATCTATACACATTGGCGGAAGACTTTTTTTCTAGGGTCTTT
  TTAATCGCTCCAGACACGGTTTCGACTCCTACTCATGGGGGCGCACACATCTGCTCTTAC
  CGTTAATTGCCCTCCGGTCAATTTCTGTTCTCTCTCCAAATATCTCGTCCCAACACCACC
  CCTCCAATTCATTATTCTCCCGNTCACGATCCCTCTGTCTCCCGCCGCATCCCCTCATCC
  TCCCATACTCATTTGCCTCCNTTACTACCCGTCACTGTTCCCTCAATCCTCNCACCCAAT
  TCCACCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017454
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017454.1, NP_059348.1
• RefSeq Size: 3142 bp
• RefSeq ORF: 1491 bp
• Locus ID: 6780
• Cytogenetics: 20q13.13
• Domains: DSRM
• Gene Summary: 'Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]'
  Transcript Variant: This variant (T1) lacks an exon in the 5' UTR compared to T2, the most abundantly expressed transcript variant. T1 encodes the same isoform (a) of 496 amino acids as T2.