TFIIS (TCEA2) (NM_003195) Human Untagged Clone

CAT#: SC109826

TCEA2 (untagged)-Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCEA2
• Synonyms: TFIIS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003195, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACCAAGAAGAGCG
  CGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCCAC
  CCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAG
  TCTCTCATCAAGTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCA
  TGCCTCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCC
  CAGGGCACCGTCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC
  AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCG
  AGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAGAA
  CCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGT
  GGGGCCATAACACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGA
  TCCGTAAGGCCATGACCAAGGAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGA
  CCTGTTCACCTGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT
  GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003195 unedited
  ATTAAAACACGACTCACTATAGGCGGACGCCAATTCGGCACGAGGCGGGTGTGGGAGGTG
  GCGACGGCCGGGGCCGGGGTCCTGCCCGGCTGCGGAGGCGGGCGCGACGGGCGCGGGGGT
  CGCTGCTCCTGAGGCGATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCT
  GGACAAGATGGTGACCAAGAAGATCTTTTTTGGAGCCATGGATTTGCTGCGGGAGCTGAA
  GGCCATGCCTATCACGCTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGC
  CCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTC
  CTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCC
  TCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGA
  GCTGCCCAGGGCACCGTCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTG
  TGATGCCGTGCGCAACAAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGA
  CCACGTGGCCATCGGTGCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTT
  CCGGGACGTTGGAAACACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAA
  CCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACC
  CCAGCAGATCGCTGTGATGACCTCAGAGAGATGGCCAGTGATGAGCTGAAGGAGATCCCG
  TAGGCCATGACCAAGGNAGCCATCCGAGAGCACCAGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003195 unedited
  AAGCTTGGACCGCGTGCCGCAATCTTANAGTCGAGTTTTTTTTTTTTTTTTTTGGCAAAA
  AGAACATTTAATAATGAGGGGGAAAGGGCAGAAAGGTGCAATCCAGGCAGGCAGGTTGAG
  GGCAGGACATGCCGCCTTCTAGGGTCTCCAGAGAAGCTGTGTCAACGGAGGACGTGGGCC
  GGGGAGGGCCCAAGGCTGCAGCACATCTACACGAGGGGTCAGCAGAACTTCCAGCGGTTT
  CCACACTCGTTGCAGACAACAAAGGTGGTCATGGGCTCATCAGAGCTGCGGGTCTGCACC
  TGTGTGTAGGTGCAGTTCTTTTTCCTGCACTTGCCGCAGGTGAACAGGTCTGTCTGCGTG
  CCGCCAGTGCGGGCCATCTGGTGCTCTCGGATGGCCTCCTTGGTCATGGCCTTACGGATC
  TCCTTCAGCTCATCACTGGCCATCTCCTCTGAGGTCATCACAGCGATCTGCTGGGGTGTT
  ATGGCCCCACACAGCACATTCCGCCGCAGGTCCCGGCTCTTGGGATCCTTCAGGCTGGAG
  ATACGACTCCGTACACGGGTCTTATACTTCATGTCTGTGTTTCCACGTCCCGGAAGAAGA
  TTCCTTGATCCGACCCGACAGGCGCCTCGAGCCCGCACCGAGGGCCACGTGGCTCTGGTC
  CCGCCGTAGGGCCACGGTTCACTATCTCCCGCGCCTTGGTGTGCCCCGGCTTTCCGGCGA
  CCAGCCCCCGGAGGAAATGTGGTGAACTTCTTGGGCCGACGGGGCCCGGGCACCTCCGCC
  CCCCGGGGCCGGGCACCCCGGCCCCCGGGCCCTCTTCAGGGCCCGCCCCAAGCATTCCAC
  TCCCCGCTCCCGGCTTCGCCCCCCGATCCTCCAATCCCCCCCCCCACCCCCGAGAACCCC
  TGGCCCCGCCCAGACCACCCCTCCCCCTCCTGTTCTCAACGCCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003195
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003195.4, NP_003186.1
• RefSeq Size: 1749 bp
• RefSeq ORF: 900 bp
• Locus ID: 6919
• Cytogenetics: 20q13.33
• Domains: TFIIS, TFS2N, TFS2M
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (a).