TBX3 (NM_016569) Human Untagged Clone

CAT#: SC109827

TBX3 (untagged)-Human T-box 3 (TBX3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX3
• Synonyms: TBX3-ISO; UMS; XHL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109827 sequence for NM_016569 edited (data generated by NextGen Sequencing)
  ATGAGCCTCTCCATGAGAGATCCGGTCATTCCTGGGACAAGCATGGCCTACCATCCGTTC
  CTACCTCACCGGGCGCCGGACTTCGCCATGAGCGCGGTGCTGGGTCACCAGCCGCCGTTC
  TTCCCCGCGCTGACGCTGCCTCCCAACGGCGCGGCGGCGCTCTCGCTGCCGGGCGCCCTG
  GCCAAGCCGATCATGGATCAATTGGTGGGGGCGGCCGAGACCGGCATCCCGTTCTCCTCC
  CTGGGGCCCCAGGCGCATCTGAGGCCTTTGAAGACCATGGAGCCCGAAGAAGAGGTGGAG
  GACGACCCCAAGGTGCACCTGGAGGCTAAAGAACTTTGGGATCAGTTTCACAAGCGGGGC
  ACCGAGATGGTCATTACCAAGTCGGGAAGGCGAATGTTTCCTCCATTTAAAGTGAGATGT
  TCTGGGCTGGATAAAAAAGCCAAATACATTTTATTGATGGACATTATAGCTGCTGATGAC
  TGTCGTTATAAATTTCACAATTCTCGGTGGATGGTGGCTGGTAAGGCCGACCCCGAAATG
  CCAAAGAGGATGTACATTCACCCGGACAGCCCCGCTACTGGGGAACAGTGGATGTCCAAA
  GTCGTCACTTTCCACAAACTGAAACTCACCAACAACATTTCAGACAAACATGGATTTACT
  TTGGNNNTCCCAAGTGATCACGCTACGTGGCAGGGGAATTATAGTTTTGGTACTCAGACT
  ATATTGAACTCCATGCACAAATACCAGCCCCGGTTCCACATTGTAAGAGCCAATGACATC
  TTGAAACTCCCTTATAGTACATTTCGGACATACTTGTTCCCCGAAACTGAATTCATCGCT
  GTGACTGCATACCAGAATGATAAGATAACCCAGTTAAAAATAGACAACAACCCTTTTGCA
  AAAGGTTTCCGGGACACTGGAAATGGCCGAAGAGAAAAAAGAAAACAGCTCACCCTGCAG
  TCCATGAGGGTGTTTGATGAAAGACACAAAAAGGAGAATGGGACCTCTGATGAGTCCTCC
  AGTGAACAAGCAGCTTTCAACTGCTTCGCCCAGGCTTCTTCTCCAGCCGCCTCCACTGTA
  GGGACATCGAACCTCAAAGATTTATGTCCCAGCGAGGGTGAGAGCGACGCCGAGGCCGAG
  AGCAAAGAGGAGCATGGCCCCGAGGCCTGCGACGCGGCCAAGATCTCCACCACCACGTCG
  GAGGAGCCCTGCCGTGACAAGGGCAGCCCCGCGGTCAAGGCTCACCTTTTCGCTGCTGAG
  CGGCCCCGGGACAGCGGGCGGCTGGACAAAGCGTCGCCCGACTCACGCCATAGCCCCGCC
  ACCATCTCGTCCAGCACTCGCGGCCTGGGCGCGGAGGAGCGCAGGAGCCCGGTTCGCGAG
  GGCACAGCGCCGGCCAAGGTGGAAGAGGCGCGCGCGCTCCCGGGCAAGGAGGCCTTCGCG
  CCGCTCACGGTGCAGACGGACGCGGCCGCCGCGCACCTGGCCCAGGGCCCCCTGCCTGGC
  CTCGGCTTCGCCCCGGGCCTGGCGGGCCAACAGTTCTTCAACGGGCACCCGCTCTTCCTG
  CACCCCAGCCAGTTTGCCATGGGGGGCGCCTTCTCCAGCATGGCGGCCGCTGGCATGGGT
  CCCCTCCTGGCCACGGTTTCTGGGGCCTCCACCGGTGTCTCGGGCCTGGATTCCACGGCC
  ATGGCCTCTGCCGCTGCGGCGCAGGGACTGTCCGGGGCGTCCGCGGCCACCCTGCCCTTC
  CACCTCCAGCAGCACGTCCTGGCCTCTCAGGGCCTGGCCATGTCCCCTTTCGGAAGCCTG
  TTCCCTTACCCCTACACGTACATGGCCGCAGCGGCGGCCGCCTCCTCTGCGGCAGCCTCC
  AGCTCGGTGCACCGCCACCCCTTCCTCAATCTGAACACCATGCGCCCGCGGCTGCGCTAC
  AGCCCCTACTCCATCCCGGTGCCGGTCCCGGACGGCAGCAGTCTGCTCACCACCGCCCTG
  CCCTCCATGGCGGCGGCCGCGGGGCCCCTGGACGGCAAAGTCGCCGCCCTGGCCGCCAGC
  CCGGCCTCGGTGGCAGTGGACTCGGGCTCTGAACTCAACAGCCGCTCCTCCACGCTCTCC
  TCCAGCTCCATGTCCTTGTCGCCCAAACTCTGCGCGGAGAAAGAGGCGGCCACCAGCGAA
  CTGCAGAGCATCCAGCGGTTGGTTAGCGGCTTGGAAGCCAAGCCGGACAGGTCCCGCAGC
  GCGTCCCCGTAG
  
  Clone variation with respect to NM_016569.3
  665 c=>n;666 c=>n;667 t=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016569 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACCAGCGAGAAAGA
  GGAGAGGAAGACAGATAGGGGGCGGGGGAAGAAGAAAAAGAAAGGTAAAAAGTCTTCTAG
  GAGAACCTTTCACATTTGCAACAAAAGACCTAGGGGCTGGAGAGAGATTCCTGGGACGCA
  GGGCTGGAGTGTCTATTTCGAGCTCAGCGGCAGGGCTCGGGCGCGAGTCGAGACCCTGCT
  CGCTCCTCTCGCTTCTGAAACCGACGTTCAGGAGCGGCTTTTTAAAAACGCAAGGCACAA
  GGACGGTCACCCGCGCGACTATGTTTGCTGATTTTTCGCCTTGCCCTCTTTAAAAGCGGC
  CTCCCATTCCCCAAAAGACACTTCCCCTCCTCCCTTTGAAGTGCATTAGTTGTGATTTCT
  GCCTCCTTTTCTTTTTTCTTTCTTTTTTGTTTTGCTTTTTCCCCCCTTTTGAATTATGTG
  CTGCTGTTAAACAACAACAAAAAAACAACAAAACACAGCAGCTGCGGACTTGTCCCCGGC
  TGGAGCCCAGCGCCCCGCCTGGAGTGGATGAGCCTCTCCATGAGAGATCCGGTCATTCCT
  GGGACAAGCATGGCCTACCATCCGTTCCTACCTCACCGGGCGCCGGACTTCGCCATGAGC
  GCGGTGCTGGGTCACCAGCCGNCGTTCTTCCCCGCGCTGACGCTGCCTCCCAACGGCGCG
  GCGGCGCTCTCGCTGNCGGGCGCCCTGGCCAGCCGATCATGGATCAATTGGGGGGGGGCG
  GCCGAGACCGGCATCCCCGTCTCTCCCTGGGGCCCAGGCGCATCTGAGCCCTTTGAGACA
  TGGAGCCCGANAAAAGGTGGAAGACGACCCCAGTGGCACTGGAGCTAAGAACTTGGGGAC
  AGTTTACAAGCGGGGCCGGGATGGCATAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016569 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTGGTTCCACTTGA
  CACAGTGAAGGAAAAATATATATATAAATCCGCACTGAGGGAGATGTCTTTGAACACCTC
  CCCGCCTGGTGGGCAGAGACCCAGACCAGCCTTGCTGGAAGTTGCTCTGGACATAAATGT
  TGGAACTCCTACCCCCAGTAGCTCAATGCAACCGACGTTTCTGAGCCCCCAGAATCTGAT
  CCAGATCCCGGACATATAAACCACGCCAAGAAGACAGGGGCTGTCTCTAGCACATTCTCT
  CGAGGGAGTCCGGGGCCCCTTCCCAGACGCAACTGCAAAAGGAAGGGCTAACGCCATGGC
  GGGCCCGAGGTTTATTTTATATCCGACAAAGTGCACGGCAGCCTGAACTGGACTGGAATG
  AAAAGACGTGTCTGGGACCGGTCTACGGGGACCCTCCGCGGGACCTGCCCCGCTTGGCTT
  TCAATCCGCTAACCCCCCCCCGGACGCTCTGTCACTTCTCTGTCGCCCGCTCTTTTTCCC
  CCCCCAGAATTCTGCTCCACTCAGCCCTTTTCTCTGTTACACCATCGCCCACGTTCTCCC
  TTTTACTTCTCACCCCCGTTCCCACTCCCCTATATCTCTGTTCCTCCCCCCTCCACTTTC
  TTTTCCCCTCCCTCTCTCCCCCCTCTACTTTTTCATTCACTGATCCCCCTTTCCTCCCCC
  ACCCGCGTATTCCACAACCCATTCTTCTCTCCTCCCCTATTCCCCCTCCCCCCCTCCCCA
  CCTCCAANAGTCAATCTCGCATGCCTTCACTCTTTCTCACTTCACTTCCTCATACCCTCG
  TTTTCTCTTCCCGTCTTTTTACACTACCCTCCTCTCACTCCTTCTCTTCGCCTCCTCCGC
  TCTCCTTATCCTCTGCATATGCTCTCTCTAATCCCCATACCACATTACTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016569
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016569.3, NP_057653.3
• RefSeq Size: 4814 bp
• RefSeq ORF: 2232 bp
• Locus ID: 6926
• Cytogenetics: 12q24.21
• Domains: T-box
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini and is longer compared to isoform 1.