Rad6 (UBE2A) (NM_003336) Human Untagged Clone

CAT#: SC109859

UBE2A (untagged)-Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2A
• Synonyms: HHR6A; MRXS30; MRXSN; RAD6A; UBC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109859 sequence for NM_003336 edited (data generated by NextGen Sequencing)
  ATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCT
  CCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATT
  TTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACT
  GAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAAT
  GTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTAT
  GATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGT
  CCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGT
  GTTTCTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGA
  
  Clone variation with respect to NM_003336.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003336 unedited
  GTCAAGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGACTTCGGA
  GAGGCAGCGCGGTTCCTCTGGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCCAGCCTCTT
  CGGCCTCCTCGCCCGCCGCGGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCG
  CTCGCGACATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGG
  AGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACG
  CGGTCATTTTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAG
  AATTCACTGAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCC
  ATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTC
  CAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATC
  CCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACANACGGGAATATG
  AAAAGCGTGTTTCTGNCATAGTAGAACAAAGCTGGCGTGATTGTTGACCCCCGGTACAGT
  TTAAAGAAAGCTGCCCATAGAAAATATATATNTGATGTGTTGTCACCTCCCTACTNCTGT
  CATTACATTTACTTTATTAAAAGCAAAATAACTGGTGTGCTGTTTCCCATCTTCTTGCCA
  AGTTTTCCTACCCCTCTACCCTCTTCTTAACATCAGAAACAACCTCTTTGAAAATCAATG
  TACTGTACCCTGGTTACTCTGGCAAAAATAACTAAGGCTCCAGTTTTTNCGGGTGAATTC
  CATTTCCAAGTTTCCAGGCCGTTATTTTTATTATTGACCTTAAGGCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003336 unedited
  TTTTTTTTTTTTTTTATGAAATTTTAAGGTTATTTTTATTTACAACTTTTGAAAAAGTAC
  ATTTTTTTTTACATGGGTTACTTGTGCAAAGTTAGATTTGGAAGTGATAAATGCATAAAA
  GGTGACAATAGAACATTAGACAAAACATTTACAAGCCTTGTCCCATACTGCTACTTAAAG
  GTACTATATATCTAAAAGTATAAATATCCAAAAAAAGATCGCAGACATTGGCTTTAAGGT
  TCTCAGATGCTGAAAGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003336
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003336.2, NP_003327.2
• RefSeq Size: 1799 bp
• RefSeq ORF: 459 bp
• Locus ID: 7319
• Cytogenetics: Xq24
• Domains: UBCc
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).