UBE3A (NM_130838) Human Untagged Clone

CAT#: SC109872

UBE3A (untagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE3A
• Synonyms: ANCR; AS; E6-AP; EPVE6AP; HPVE6A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_130838, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATG
  AAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTAT
  TAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGC
  TCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAG
  GCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATG
  TAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTG
  GTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAG
  ACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATC
  TTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCT
  GTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTC
  TCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGA
  TCCTAATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAA
  ATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGAC
  TGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAA
  AGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAG
  TGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATG
  ATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAA
  GAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTT
  ATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTT
  TCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAA
  TTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTA
  GTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCAC
  TTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGA
  AGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTC
  AATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTG
  AAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGA
  TGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGAC
  TCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGA
  TGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGG
  TGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAAT
  AAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAA
  AGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACT
  AGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATC
  GTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTG
  TGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATC
  TCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTG
  AAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130838 unedited
  GCGGAAANCACCATTCGCACGAGGCTGATTTTATATTTCACCAGCTCCTCAGAATTTGGC
  GAAATATGAGTTATTAAGCCTACGCTCAGATCAAGGTAGCAGCTAGACTGGTGTGACAAC
  CTGTTTTTAATCAGTGACTCAAAGCTGTGATCACCCTGATGTCACCGAATGGCCACAGCT
  TGNAATAGATAGTTACAGTGGAGGTAAAAGGAGTGGCTTGCAGGATGGAGAAGCTGCACC
  AGTGTTATTGGAATGCCAGCAGGTTTATTTTTTGTTTTGCAAGCCAGCTCTGCCTCCTTA
  CAGTATGACATCTGATGCTGGAGGGTCGCACTTTCAAAAATGAGTCAGCTGGTACATGGG
  GTTATCATCAATTTTTAGCTCTTCTGTCTGGGAGATACAAGTTTGGAAGCAATCTTGGGG
  TACTTACCCACAAGGCTGGTGGAGACCAGATCAGGAGAACCTCAGTCTGACGACATTGAA
  GCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTA
  ACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTT
  CTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCA
  AAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCG
  AAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAATGAACAAGAAAGGCGCTAGAATTGA
  TTTTAAAGATGTGACTTACTTNACAGAAAGAAAGGTATATGAAATTCTTTGATATGTAGA
  GAAAGAGAGGATTATTCCCTTTATCCGTGTTATTGGAAGAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130838 unedited
  TTTTNAAAANNNNNTTATCTNTGNACCGCGGNNCCGCATTCTNANGATCGAGTTTTTTTT
  TTTTTTTTTTGGTTTATTTTGTTTTGTTTTGTTTTACAGCATGCCAAATCCTTTGGCATA
  CGTGATGGCCTTCAACAATCTCTCTTTAAGTTTTTCTTTGCTTGAGTATTCCGGAAGTAA
  AAGCACATTAAAGCAAGTATGAGATGTAGGTAACCTTTCTGTGTCTGGGCCATTTTTGGC
  TATAATCATCTTTAATTTTCCTAGTCCTCCCACAGGTGCTCTGTCTGTGCCCGTTGTAAA
  CTGCAAGAAGAGTCTTTTCCTGTTCATCTGTAAATGAATGAACGATTTCCCAGAACTCCC
  TAATCAGAACAGAGTCCCTGGTATAGCCACCGTCATATTCTGTAGGTTCTTCTAGTGCTT
  GGAAAATCTAGATCCGGCTTNCACATANTAGCANTNCAATTNCTTCTGGTCTGAATAAGT
  ACTTTAAGGGAGGATCATTGGTCACCATATGAAAACCCTCTCGAAAAGCCTTGAACTNGT
  TTTCTACTGATTTATTGAGAATGAGTCAGAATAAAGATGACAAAATCCTTCTGGGTTCAT
  TTGAATTGGAATTTTATCACCATTTTCCTTAAAACATACATCATTGGTTACCAAAAAAAT
  CTGGCCGGGATATTTGGAAAGGACATCATGGATTTTCCCATTCCCTTTTAACTCCATAAA
  ACTTTAAACCTTGATTTAAACCTGGGGAAAGTTTCCCAGTAACGAAAAGTCCCTTTTTCC
  CATTACCTCCTGGAAACACCCTGGGAAAGGCCCTCCCGTTCCGGTTTTGAAAAAGCCAAC
  CCCCTCTTGCCCATCAAAAAACTGCCCCTCGTTTCAAAAAAATGGTAACCCAAACATTTG
  GAAATTCCGGGGGAACCACCCAATCGGTTGAAATTCCCCCCAACCCTAAAAATTTTGGAA
  ACCTCCTTAAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130838
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130838.1, NP_570853.1
• RefSeq Size: 4491 bp
• RefSeq ORF: 2559 bp
• Locus ID: 7337
• Cytogenetics: 15q11.2
• Domains: HECT
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) uses a downstream in-frame start codon, as compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.